Proteomics is the study of the proteome, the full set of proteins expressed in an organism or system. Proteomics is used to investigate not only the function and structure of proteins, but also how they interact with each other inside cells, how they are modified, where they’re located in cells and how they respond to drugs and disease.
Cambridge Centre for Proteomics
The Cambridge Centre for Proteomics in the School of Biological Sciences provides a considerable amount of expertise in proteomics and mass spectrometry-based techniques to both academic and industrial researchers.
Experiments are performed using state-of–the-art instrumentation, which includes a range of nanoLC systems coupled with Orbitrap/timsTOF mass spectrometers. The facility currently offers full quantitative analysis on virtually any sample of any complexity.
Services
- Protein Identification by Mass Spectrometry, including:
- Full sample processing, including reduction, alkylation and enzymatic digestion of proteins within the gel bands or solutions.
- High resolution reverse-phase chromatography followed by high mass accuracy MS/MS.
- Mascot database searching and interpretation of data using standard or custom databases.
- High-Throughput Protein Quantitation by Mass Spectrometry, including:
- Full sample preparation including protein estimation, reduction, alkylation, digestion and TMT (6-plex to 18-plex) labelling.
- We do not currently provide SILAC labelling but we can process SILAC-labelled samples.
- Post-translational modification (PTM) mapping and synthetic modifications mapping of non-complex samples, including:
- Full sample preparation.
- LC-MS/MS experiments.
- Full data analysis including assignment of PTM sites, where possible.