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Department of Biochemistry


Our pubmed entries


Holzer S, Yan J, Kilkenny ML, Bell SD, Pellegrini L. Primer synthesis by a eukaryotic-like archaeal primase is independent of its Fe-S cluster. Nat Commun. 2017 Nov 23;8(1):1718. doi: 10.1038/s41467-017-01707-w. PubMed PMID: 29167441.

Kilkenny ML, Simon AC, Mainwaring J, Wirthensohn D, Holzer S, Pellegrini L. The human CTF4-orthologue AND-1 interacts with DNA polymerase α/primase via its unique C-terminal HMG box. Open Biol. 2017 Nov;7(11). pii: 170217. doi: 10.1098/rsob.170217. PubMed PMID: 29167311.

Wu Y, Villa F, Maman J, Lau YH, Dobnikar L, Simon AC, Labib K, Spring DR, Pellegrini L. Targeting the Genome-Stability Hub Ctf4 by Stapled-Peptide Design. Angew Chem Int Ed Engl. 2017 Oct 9;56(42):12866-12872. doi:10.1002/anie.201705611. Epub 2017 Sep 7. PubMed PMID: 28815832.

Kolinjivadi AM, Sannino V, De Antoni A, Zadorozhny K, Kilkenny M, Técher H, Baldi G, Shen R, Ciccia A, Pellegrini L, Krejci L, Costanzo V. Smarcal1-Mediated  Fork Reversal Triggers Mre11-Dependent Degradation of Nascent DNA in the Absence  of Brca2 and Stable Rad51 Nucleofilaments. Mol Cell. 2017 Sep 7;67(5):867-881.e7. doi: 10.1016/j.molcel.2017.07.001. Epub 2017 Jul 27. PubMed PMID: 28757209; PubMed Central PMCID: PMC5594205.

Pellegrini L. Comment on "The [4Fe4S] cluster of human DNA primase functions as a redox switch using DNA charge transport". Science. 2017 Jul 21;357(6348). pii: eaan2954. doi: 10.1126/science.aan2954. PubMed PMID: 28729486.

Pellegrini L. Structural insights into Cdc45 function: was there a nuclease at the heart of the ancestral replisome? Biophys Chem. 2017 Jun;225:10-14. doi:10.1016/j.bpc.2016.11.011. Epub 2016 Nov 27. Review. PubMed PMID: 27919598; PubMed Central PMCID: PMC5484177.

Holzer S, Degliesposti G, Kilkenny ML, Maslen SL, Matak-Vinkovíc D, Skehel M, Pellegrini L. Crystal structure of the N-terminal domain of human Timeless and its interaction with Tipin. Nucleic Acids Res. 2017 May 19;45(9):5555-5563. doi: 10.1093/nar/gkx139. PubMed PMID: 28334766; PubMed Central PMCID: PMC5605233.

Syrjänen JL, Heller I, Candelli A, Davies OR, Peterman EJ, Wuite GJ, Pellegrini L. Single-molecule observation of DNA compaction by meiotic protein SYCP3. Elife. 2017 Mar 13;6. pii: e22582. doi: 10.7554/eLife.22582. PubMed PMID: 28287952; PubMed Central PMCID: PMC5348128.


Pellegrini L, Costa A. New Insights into the Mechanism of DNA Duplication by the Eukaryotic Replisome. Trends Biochem Sci. 2016 Oct;41(10):859-71. doi: 10.1016/j.tibs.2016.07.011. Epub 2016 Aug 20. Review. PubMed PMID: 27555051.

Simon AC, Sannino V, Costanzo V, Pellegrini L. Structure of human Cdc45 and implications for CMG helicase function. Nat Commun. 2016 May 18;7:11638. doi: 10.1038/ncomms11638. PubMed PMID: 27189187; PubMed Central PMCID: PMC4873980.

Villa F, Simon AC, Ortiz Bazan MA, Kilkenny ML, Wirthensohn D, Wightman M, Matak-Vinkovíc D, Pellegrini L, Labib K. Ctf4 Is a Hub in the Eukaryotic Replisome that Links Multiple CIP-Box Proteins to the CMG Helicase. Mol Cell. 2016 Aug 4;63(3):385-96. doi: 10.1016/j.molcel.2016.06.009. Epub 2016 Jul 7. PubMed PMID: 27397685; PubMed Central PMCID: PMC4980431.


Anjum RS, Bray SM, Blackwood JK, Kilkenny ML, Coelho MA, Foster BM, Li S, Howard JA, Pellegrini L, Albers SV, Deery MJ, Robinson NP. Involvement of a eukaryotic-like ubiquitin-related modifier in the proteasome pathway of the archaeon Sulfolobus acidocaldarius. Nat Commun. 2015 Sep 8;6:8163. doi: 10.1038/ncomms9163. PubMed PMID: 26348592; PubMed Central PMCID: PMC4569737.

Forment JV, Jackson SP, Pellegrini L. When two is not enough: a CtIP tetramer is required for DNA repair by Homologous Recombination. Nucleus. 2015;6(5):344-8. doi: 10.1080/19491034.2015.1086050. PubMed PMID: 26305173; PubMed Central PMCID:  PMC4915501.

Davies OR, Forment JV, Sun M, Belotserkovskaya R, Coates J, Galanty Y, Demir M, Morton CR, Rzechorzek NJ, Jackson SP, Pellegrini L. CtIP tetramer assembly is required for DNA-end resection and repair. Nat Struct Mol Biol. 2015 Feb;22(2):150-157. doi: 10.1038/nsmb.2937. Epub 2015 Jan 5. PubMed PMID: 25558984; PubMed Central PMCID: PMC4564947.


Syrjänen JL, Heller I, Candelli A, Davies OR, Peterman EJ, Wuite GJ, Pellegrini L. Single-molecule observation of DNA compaction by meiotic protein SYCP3. Elife. 2017 Mar 13;6. pii: e22582. doi: 10.7554/eLife.22582. PubMed PMID: 28287952; PubMed Central PMCID: PMC5348128.

Rzechorzek NJ, Blackwood JK, Bray SM, Maman JD, Pellegrini L, Robinson NP. Structure of the hexameric HerA ATPase reveals a mechanism of translocation-coupled DNA-end processing in archaea. Nat Commun. 2014 Nov 25;5:5506. doi: 10.1038/ncomms6506. PubMed PMID: 25420454; PubMed Central PMCID: PMC4376295.

Syrjänen JL, Pellegrini L, Davies OR. A molecular model for the role of SYCP3 in meiotic chromosome organisation. Elife. 2014 Jun 20;3. doi: 10.7554/eLife.02963. PubMed PMID: 24950965; PubMed Central PMCID: PMC4102245.

Simon AC, Zhou JC, Perera RL, van Deursen F, Evrin C, Ivanova ME, Kilkenny ML, Renault L, Kjaer S, Matak-Vinković D, Labib K, Costa A, Pellegrini L. A Ctf4 trimer couples the CMG helicase to DNA polymerase α in the eukaryotic replisome. Nature. 2014 Jun 12;510(7504):293-297. doi: 10.1038/nature13234. Epub 2014 May 4. PubMed PMID: 24805245; PubMed Central PMCID: PMC4059944.


Kilkenny ML, Longo MA, Perera RL, Pellegrini L. Structures of human primase reveal design of nucleotide elongation site and mode of Pol α tethering. Proc Natl Acad Sci U S A. 2013 Oct 1;110(40):15961-6. doi: 10.1073/pnas.1311185110. Epub 2013 Sep 16. PubMed PMID: 24043831; PubMed Central PMCID: PMC3791718.

Perera RL, Torella R, Klinge S, Kilkenny ML, Maman JD, Pellegrini L. Mechanism for priming DNA synthesis by yeast DNA polymerase α. Elife. 2013 Apr 2;2:e00482. doi: 10.7554/eLife.00482. PubMed PMID: 23599895; PubMed Central PMCID: PMC3628110.

Blackwood JK, Rzechorzek NJ, Bray SM, Maman JD, Pellegrini L, Robinson NP. End-resection at DNA double-strand breaks in the three domains of life. Biochem Soc Trans. 2013 Feb 1;41(1):314-20. doi: 10.1042/BST20120307. PubMed PMID: 23356304; PubMed Central PMCID: PMC3561678.


Pellegrini L. The Pol α-primase complex. Subcell Biochem. 2012;62:157-69. doi: 10.1007/978-94-007-4572-8_9. Review. PubMed PMID: 22918585.

Davies OR, Maman JD, Pellegrini L. Structural analysis of the human SYCE2-TEX12 complex provides molecular insights into synaptonemal complex assembly. Open Biol. 2012 Jul;2(7):120099. doi: 10.1098/rsob.120099. PubMed PMID: 22870393; PubMed Central PMCID: PMC3411106.

Kilkenny ML, De Piccoli G, Perera RL, Labib K, Pellegrini L. A conserved motif in the C-terminal tail of DNA polymerase α tethers primase to the eukaryotic replisome. J Biol Chem. 2012 Jul 6;287(28):23740-7. doi: 10.1074/jbc.M112.368951. Epub 2012 May 16. PubMed PMID: 22593576; PubMed Central PMCID: PMC3390648.

Blackwood JK, Rzechorzek NJ, Abrams AS, Maman JD, Pellegrini L, Robinson NP. Structural and functional insights into DNA-end processing by the archaeal HerA helicase-NurA nuclease complex. Nucleic Acids Res. 2012 Apr;40(7):3183-96. doi: 10.1093/nar/gkr1157. Epub 2011 Dec 1. PubMed PMID: 22135300; PubMed Central PMCID: PMC3326311.


Sauguet L, Klinge S, Perera RL, Maman JD, Pellegrini L. Shared active site architecture between the large subunit of eukaryotic primase and DNA photolyase. PLoS One. 2010 Apr 9;5(4):e10083. doi: 10.1371/journal.pone.0010083. PubMed PMID: 20404922; PubMed Central PMCID: PMC2852410.

Klinge S, Núñez-Ramírez R, Llorca O, Pellegrini L. 3D architecture of DNA Pol alpha reveals the functional core of multi-subunit replicative polymerases. EMBO J. 2009 Jul 8;28(13):1978-87. doi: 10.1038/emboj.2009.150. Epub 2009 Jun 4. PubMed PMID: 19494830; PubMed Central PMCID: PMC2693882.

Ragone S, Maman JD, Furnham N, Pellegrini L. Structural basis for inhibition of homologous recombination by the RecX protein. EMBO J. 2008 Aug 20;27(16):2259-69. doi: 10.1038/emboj.2008.145. Epub 2008 Jul 24. PubMed PMID: 18650935; PubMed Central PMCID: PMC2500204.

Klinge S, Hirst J, Maman JD, Krude T, Pellegrini L. An iron-sulfur domain of the eukaryotic primase is essential for RNA primer synthesis. Nat Struct Mol Biol. 2007 Sep;14(9):875-7. Epub 2007 Aug 19. PubMed PMID: 17704817; PubMed Central PMCID: PMC2268749.

Davies OR, Pellegrini L. Interaction with the BRCA2 C terminus protects RAD51-DNA filaments from disassembly by BRC repeats. Nat Struct Mol Biol. 2007 Jun;14(6):475-83. Erratum in: Nat Struct Mol Biol. 2007 Jul;14(7):680. PubMed PMID: 17515903; PubMed Central PMCID: PMC2096194.

Nookala RK, Hussain S, Pellegrini L. Insights into Fanconi Anaemia from the structure of human FANCE. Nucleic Acids Res. 2007;35(5):1638-48. Epub 2007 Feb 18. PubMed PMID: 17308347; PubMed Central PMCID: PMC1865054.

Shivji MK, Davies OR, Savill JM, Bates DL, Pellegrini L, Venkitaraman AR. A region of human BRCA2 containing multiple BRC repeats promotes RAD51-mediated strand exchange. Nucleic Acids Res. 2006;34(14):4000-11. Epub 2006 Aug 16. PubMed PMID: 16914443; PubMed Central PMCID: PMC1557805.

Doré AS, Furnham N, Davies OR, Sibanda BL, Chirgadze DY, Jackson SP, Pellegrini L, Blundell TL. Structure of an Xrcc4-DNA ligase IV yeast ortholog complex reveals a novel BRCT interaction mode. DNA Repair (Amst). 2006 Mar 7;5(3):362-8. Epub 2006 Jan 18. PubMed PMID: 16388993.

Lao-Sirieix SH, Nookala RK, Roversi P, Bell SD, Pellegrini L. Structure of the heterodimeric core primase. Nat Struct Mol Biol. 2005 Dec;12(12):1137-44. Epub 2005 Nov 6. PubMed PMID: 16273105.

Lao-Sirieix SH, Pellegrini L, Bell SD. The promiscuous primase. Trends Genet. 2005 Oct;21(10):568-72. Review. PubMed PMID: 16095750.

Pellegrini L, Venkitaraman A. Emerging functions of BRCA2 in DNA recombination. Trends Biochem Sci. 2004 Jun;29(6):310-6. PubMed PMID: 15276185.


Contact details

Research Group Leader  Luca Pellegrini​


Location  Sanger Building


The Pellegrini Group is accepting enquiries from prospective interns, undergraduate students, postgraduate students and postdoctoral researchers.