Kathryn Lilley, members of the Cambridge Centre for Proteomics and collaborators publish a human cell atlas in Science.
Kathryn Lilley and members of the Cambridge Centre for Proteomics, with collaborators from the Royal Institute of Technology, KTH, Sweden publish a human cell atlas in Science, 11th May 2017.
The authors present a comprehensive image-based map of subcellular protein distribution, the Cell Atlas, built by integrating transcriptomics and antibody-based immunofluorescence microscopy with validation by mass spectrometry. Mapping the in situ localisation of 12,003 human proteins at a single-cell level to 30 subcellular structures enabled the definition of 13 major organelle proteomes. Exploration of the proteomes reveals single-cell variations of abundance or spatial distribution, and localisation of approximately half of the proteins to multiple compartments. This subcellular map can be used to refine existing protein-protein interaction networks and provides an important resource to deconvolute the highly complex architecture of the human cell.
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