skip to content

 

Department of Biochemistry

 
Department of Biochemistry news archive

Kathryn Lilley, members of the Cambridge Centre for Proteomics and collaborators publish a human cell atlas in Science.

 

Kathryn Lilley and members of the Cambridge Centre for Proteomics, with collaborators from the Royal Institute of Technology, KTH, Sweden publish a human cell atlas in Science, 11th May 2017. 

The authors present a comprehensive image-based map of subcellular protein distribution, the Cell Atlas, built by integrating transcriptomics and antibody-based immunofluorescence microscopy with validation by mass spectrometry. Mapping the in situ localisation of 12,003 human proteins at a single-cell level to 30 subcellular structures enabled the definition of 13 major organelle proteomes. Exploration of the proteomes reveals single-cell variations of abundance or spatial distribution, and localisation of approximately half of the proteins to multiple compartments. This subcellular map can be used to refine existing protein-protein interaction networks and provides an important resource to deconvolute the highly complex architecture of the human cell.

For the full news story, visit the University website.

Author

Stephanie Low

Publication date

12 May 2017