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Single Molecule Imaging

Single Molecule Imaging2
Figure: Counting CENP-A molecules at the centromere at different stages of the cell cycle

Although fluorescence microscopy has been widely employed as an imaging tool for biological studies, super–resolution fluorescence microscopy allows the 3D mapping of fluorescent proteins in cells with an accuracy far beyond the diffraction limit of visible light. We are currently exploiting super–resolution Photo Activation Localisation Microscopy (PALM) to directly image proteins involved in chromatin assembly and disassembly in both fixed and live cells at a near molecular resolution. This involves using molecular and cell biological techniques to tag particular proteins with photo–activatable fluorophores in order to study these proteins at the single molecule level. In preliminary studies we have investigated a model epigenetic process – the timing and deposition of the centromere–specific histone H3 variant CENP–A (Cnp1 in S. Pombe) during the cell cycle.

For further details see: Lando et al., Open Biol. 2012, 2(7):120078.

Members of our group currently involved in this project are: Dr David Lando, Dr Srinjan Basu, Edward Taylor, Yang Cao

Contact Details

Ernest Laue
Professor of Structural Biology

Tel: +44 (0)1223 333677
Email: e.d.laue (at)


PA: Tessa Kretschmann

Tel: +44 (0)1223 766110
Email: edlsec (at)


Mailing address:

Department of Biochemistry
University of Cambridge
Old Addenbrookes Site
80 Tennis Court Road
Cambridge, CB2 1GA


Visiting address:

Sanger Building