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Hollfelder group

Stephane Emond

Joined the lab: April 2010
Background and Current Projects: After receiving my MSc in Biochemical Engineering from INSA Toulouse (France), I did an industrial PhD (2004-2007) with a biotech company (MilleGen, Toulouse) under the academic supervision of Prof Pierre Monsan, where I worked on developing random mutagenesis approaches using error-prone DNA polymerases and engineering glycoside hydrolases (amylosucrase) using directed evolution. I then joined the group of Prof Magali Remaud-Simeon where I worked on different projects involving lipases and esterases. I joined the Hollfelder group in 2010 and my current work investigates the effects of insertions and deletions (InDels) in proteins using laboratory evolution approaches.

Selected publications

Gielen F., Hours R., Emond S., Fischlechner M., Schell U., Hollfelder F. (2016). Ultrahigh-throughput-directed enzyme evolution by absorbance-activated droplet sorting (AADS). Proc. Natl Acad. Sci USA, 113(47), E7383-E7389. read online

Kaltenbach M., Emond S., Hollfelder F., Tokuriki N. (2016). Functional trade-offs in promiscuous enzymes cannot be explained by intrinsic mutational robustness of the native activity. PLOS Genetics, 12(10): e1006305. read online

Emond S., Socha R., Tokuriki N. (2015). Strategies to Overcome Stability Constraints in Enzyme Evolution and Facilitate Effective Enzyme Engineering, in "Industrial Biocatalysis" (Edited by P. Grunwald), Pan Stanford Publishing, 115-159.

Theerachat M., Emond S., Cambon E., Bordes F., Marty A., Nicaud J.-M., Chulalaksananukul W., Guieysse D., Remaud-Siméon M., Morel S. (2012). Engineering and production of laccase from Trametes versicolor in the yeast Yarrowia lipolytica. Bioresource Technology 125, 267–274. read online

Emond S., Guieysse D., Lechevallier S., Dexpert-Ghys J., Monsan P., Remaud-Siméon M. (2012). Alteration of enzyme activity and enantioselectivity by biomimetic encapsulation in silica particles. Chemical Communications 48(9), 1314–1316. read online

Emond S., Montanier C., Nicaud J.-M., Marty A., André I., Monsan P., Remaud-Siméon M. (2010). New Efficient Recombinant Expression System To Engineer Candida antarctica Lipase B. Applied and Environmental Microbiology 76(8), pp. 2684-2687. read online

Emond S., Mondeil S., Jaziri K., André I., Monsan P., Remaud-Siméon M., Potocki-Véronèse G. (2008). Cloning, purification and characterization of a thermostable amylosucrase from Deinococcus geothermalis. FEMS Microbiology Letters, 285(1), pp. 25-32. read online

Emond S., André I., Jaziri K., Potocki-Véronèse G., Mondon P., Bouayadi K., Kharrat H., Monsan P., Remaud-Siméon M. (2008). Combinatorial engineering to enhance amylosucrase thermostability. Protein Science, 17(6), pp. 967-976. read online

Stephane Emond

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