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Hollfelder group

Katharina Neufeld

Visiting PostDoc
Joined the lab: May 2017
Background and Current Projects: My interest in biochemistry due to the pioneering nature of this field of research became a deciding criterion to study this topic and enabled me for a fast-track-Ph.D. in organic chemistry in Germany followed by a Post-Doc in the US, both of which I conducted in the area of biotechnology. In my projects I have been targeting the engineering and application of biocatalysts for the synthesis of small as well as polymeric compounds using different enzymes classes such as alcohol dehydrogenases, lipases, monooxygenases and proteases; the establishment of enzyme assays based on optical and conventional analytical techniques was a major necessity in many projects. I believe that a fundamental understanding of molecular biology, enzyme biochemistry, organic chemistry and analytics is crucial to be successful in projects at the chemistry-biology-interface. After being responsible for the set-up and maintenance of a high-throughput analytics facility in the biocatalysis unit at Johnson Matthey, I am collaborating with the Hollfelder lab to investigate the potential of droplet-based microfluidics for the evolution and identification of industrially relevant biocatalysts.
Interests: biocatalysis, enzyme engineering, assay development

Publications:

Neufeld, K., Phan, V. N., Liu, F., Dooley, A. L., Subramaniam, G., and Gross, R. A., Protease-catalyzed oligomerization of non-natural amino acids functionalized with groups useful for Click Chemistry and their prospects for hydrogels, Biomacromolecules (in preparation).

Holec, C., Neufeld, K., and Pietruszka, J., P450 BM3 monooxygenase as an efficient NAD(P)H-oxidase for regeneration of nicotinamide cofactors in ADH-catalysed preparative scale biotransformations, (2016) Adv. Synth. Catal. 358: 1810–1819. Read online

Liu, K., Zhao, X., Huo, J., Neufeld, K., Dong, M., and Zhu, X., Anion-induced optical outputs of an isatin-based colorimetric HNI sensor for construction of sequential molecular logic gates and a set-reset memorized device, (2016) Sens. Actuators, B 226: 465–470. Read online

Neufeld, K., Henßen, B., and Pietruszka, J., Enantioselektive allylische Hydroxylierung von w-Alkensäuren und -estern mittels der P450-BM3-Monooxygenase, (2014) Angew. Chem. 126: 13469–13473; Enantioselective allylic hydroxylation of w-alkenoic acids and esters by P450 BM3 monooxygenase, (2014) Angew. Chem. Int. Ed. 53: 13253–13257. Read online

Konieszny, S., Krumm, C., Doert, D., Neufeld, K., and Tiller, J. C., Investigations on the activity of poly(2-oxazoline) enzyme conjugates dissolved in organic solvents, (2014) J. Biotechnol. 181: 55–63. Read online

Neufeld, K., Meyer zu Berstenhorst, S., and Pietruszka, J., Evaluation of coumarin-based fluorogenic P450 BM3 substrates and prospects for competitive inhibition screenings, (2014) Anal. Biochem. 456: 70–81. Read online

Neufeld, K., Marienhagen, J., Pietruszka, J., and Schwaneberg, U., Benzylic hydroxylation of aromatic compounds by P450 BM3, (2013) Green Chem. 15: 2408–2421. Read online

Neufeld, K. and Pietruszka, J., Understanding nature - towards the enzymatic degradation of cellulose with monooxygenases, (2012) ChemCatChem 4: 1239–1240. Read online