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The Department's buildings are currently open for wet laboratory work only. We have carried out a comprehensive COVID-19 risk assessment process and have introduced a number of new measures to ensure the safety of our staff, including reduced building occupancy, strict social distancing, 'family'-based working, and increased cleaning and hygiene regimes. All staff who can work remotely will do so for the foreseeable future. Please continue to contact us by email until further notice.

Department of Biochemistry


Josephin Holstein

Joined the lab: January 2017
Background and Current Projects: I graduated in chemistry at the University of Hamburg. During my master’s degree I visited the Poul Nielsen group at the University of Southern Denmark for a research project based on the synthesis of double-headed nucleosides using Sonogashira-couplings. Afterwards I joined the research group of Andrea Rentmeister for my master thesis focussing on the diversification of the substrate spectrum of trimethylguanosine synthases. For my doctoral thesis I followed Andrea Rentmeister to the University of Münster to establish a chemo-enzymatic approach for labelling the 5’ cap of eukaryotic mRNAs via bioorthogonal click reactions. In January 2017 I joined the group of Florian Hollfelder as a PostDoc to work on the screening of metagenomic libraries for non-natural enzyme activities using microfluidic droplet systems.
Interests: In my free time I enjoy running, doing pilates and yoga, and I like playing the piano.


[1] J. M. Holstein,* F. Muttach,* S. H. H. Schiefelbein, and A. Rentmeister, Dual 5' cap labeling based on regioselective RNA methyltransferases and bioorthogonal reactions, Chem. – Eur. J., 2017, accepted. (*: equal contribution)

[2] J. M. Holstein, L. Anhäuser, and A. Rentmeister, Modifying the 5'-cap to click eukaryotic mRNA and tune translation efficiency in living cells, Angew. Chem. Int. Ed., 2016, 55, 10899-10903.

[3] J. M. Holstein, D. Stummer, and A. Rentmeister, Enzymatic modification of 5'-capped RNA and subsequent labeling by click chemistry, Methods Mol. Biol., 2016, 1428, 45-60.

[4] J. M. Holstein, and A. Rentmeister, Current covalent modification methods for detecting RNA in fixed and living cells, Methods, 2016, 98, 18-25.

[5] J. M. Holstein, D. Stummer, and A. Rentmeister, Engineering Giardia lamblia trimethylguanosine synthase (GlaTgs2) to transfer non-natural modifications to the RNA 5'-cap, Protein Eng. Des. Sel., 2015, 28, 179-186.

[6] J. M. Holstein,* D. Stummer,* and A. Rentmeister, Enzymatic modification of 5'-capped RNA with a 4-vinylbenzyl group provides a platform for photoclick and inverse electron-demand Diels-Alder reaction, Chem. Sci., 2015, 6, 1362-1369. (*: equal contribution)

[7] J. M. Holstein, D. Schulz, and A. Rentmeister, Bioorthogonal site-specific labeling of the 5'-cap structure in eukaryotic mRNAs, Chem. Commun., 2014, 50, 4478-4481.

[8] D. Schulz, J. M. Holstein and A. Rentmeister, A chemo-enzymatic approach for site-specific modification of the RNA cap, Angew. Chem. Int. Ed., 2013, 52, 7874-7878.


Josephin Holstein 

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