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Department of Biochemistry

 

Josephin Holstein

PostDoc
Joined the lab: January 2017
Background and Current Projects: I graduated in chemistry at the University of Hamburg. During my master’s degree I visited the Poul Nielsen group at the University of Southern Denmark for a research project based on the synthesis of double-headed nucleosides using Sonogashira-couplings. Afterwards I joined the research group of Andrea Rentmeister for my master thesis focussing on the diversification of the substrate spectrum of trimethylguanosine synthases. For my doctoral thesis I followed Andrea Rentmeister to the University of Münster to establish a chemo-enzymatic approach for labelling the 5’ cap of eukaryotic mRNAs via bioorthogonal click reactions. In January 2017 I joined the group of Florian Hollfelder as a PostDoc to work on the screening of metagenomic libraries for non-natural enzyme activities using microfluidic droplet systems.
Interests: In my free time I enjoy running, doing pilates and yoga, and I like playing the piano.

Publications

[1] J. M. Holstein,* F. Muttach,* S. H. H. Schiefelbein, and A. Rentmeister, Dual 5' cap labeling based on regioselective RNA methyltransferases and bioorthogonal reactions, Chem. – Eur. J., 2017, accepted. (*: equal contribution)

[2] J. M. Holstein, L. Anhäuser, and A. Rentmeister, Modifying the 5'-cap to click eukaryotic mRNA and tune translation efficiency in living cells, Angew. Chem. Int. Ed., 2016, 55, 10899-10903.

[3] J. M. Holstein, D. Stummer, and A. Rentmeister, Enzymatic modification of 5'-capped RNA and subsequent labeling by click chemistry, Methods Mol. Biol., 2016, 1428, 45-60.

[4] J. M. Holstein, and A. Rentmeister, Current covalent modification methods for detecting RNA in fixed and living cells, Methods, 2016, 98, 18-25.

[5] J. M. Holstein, D. Stummer, and A. Rentmeister, Engineering Giardia lamblia trimethylguanosine synthase (GlaTgs2) to transfer non-natural modifications to the RNA 5'-cap, Protein Eng. Des. Sel., 2015, 28, 179-186.

[6] J. M. Holstein,* D. Stummer,* and A. Rentmeister, Enzymatic modification of 5'-capped RNA with a 4-vinylbenzyl group provides a platform for photoclick and inverse electron-demand Diels-Alder reaction, Chem. Sci., 2015, 6, 1362-1369. (*: equal contribution)

[7] J. M. Holstein, D. Schulz, and A. Rentmeister, Bioorthogonal site-specific labeling of the 5'-cap structure in eukaryotic mRNAs, Chem. Commun., 2014, 50, 4478-4481.

[8] D. Schulz, J. M. Holstein and A. Rentmeister, A chemo-enzymatic approach for site-specific modification of the RNA cap, Angew. Chem. Int. Ed., 2013, 52, 7874-7878.

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Josephin Holstein 

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