skip to primary navigationskip to content
 

Specific techniques

Our group is developing diverse techniques in order to identify and characterise candidate genes involved in polysaccharide synthesis, and in order to characterise and quantify the structure of cell wall polysaccharides. Many of the techniques are developed in collaboration with other research laboratories.

Localization of Organelle Proteins by Isotope Tagging LOPIT:
Quantitative proteomics to identify the main polysaccharide synthase enzymes in the plant Golgi apparatus (Dunkley et al., 2004;Dunkley et al., 2006).
This technique was developed in collaboration with Dr Kathryn Lilley.

Proteomic Complex Detection using Sedimentation ProCoDeS:
Quantitative proteomics to detect endogenous protein complexes (Hartman et al., 2007Segura at el., 2010).
This technique was developed in collaboration with Dr Kathryn Lilley.

Oligosaccharide relative Quantitation Using Isotope Tagging OliQuiT:
Relative quantification of oligosaccharides using normal-phase liquid chromatography/mass spectrometry (Ridlova et al., 2008).
This technique was developed in collaboration with Dr. Elaine Stephens.

Polysaccharide Analysis by Carbohydrate gel Electrophoreses PACE:
A simple quantitative method to characterize enzymatically digested polysaccharides (Goubet et al., 2003).

DNA Sequencer Assisted Saccharide analysis in Highthroughput DASH:
A fast method to characterize enzymatically digested polysaccharides (Li et al. 2013).

Solution Nuclear Magnetic Resonance NMR:
NMR can be used to characterise polysaccharide structures in solution (Busse-Wicher et al., 2014; Tryfona, et al, 2014). Our work is in collaboration with Dr Katherine Stott.

Solid-state Nuclear Magnetic Resonance NMR:
Is a variant of the NMR spectroscopy used to characterise molecular structures and conformations in solids, in our case polysaccharide structures in the plant cell wall, avoiding extraction of the polysaccharides (Dupree et al., 2015). This technique was developed in collaboration with Prof Ray Dupree and Prof Steven Brown.