skip to content

 

The Department's buildings are currently open for wet laboratory work only. We have carried out a comprehensive COVID-19 risk assessment process and have introduced a number of new measures to ensure the safety of our staff, including reduced building occupancy, strict social distancing, 'family'-based working, and increased cleaning and hygiene regimes. All staff who can work remotely will do so for the foreseeable future. Please continue to contact us by email until further notice.

Department of Biochemistry

 

The Department has conducted a comprehensive COVID-19 risk assessment process and has introduced a number of new measures to ensure the safety of our staff. In order to accommodate these new measures, our facilities will be operating revised services for the foreseeable future. Details of current arrangements and available services can be found here.

Stock Primers

We supply some primers for use in sequencing reactions.

We also accept any custom primer. Please design your sequencing primers to be between 18 and 22 bases long. Each primer should have a GC content between 50 and 60%. The primer annealing temperature should be between 55 and 60°C.

Please supply your custom primers at a concentration of 10pm/µl (10µM) in water. We use 2µl of primer solution per reaction but please give us an excess to allow for evaporation or any other potential loss.

Stock Primers

Primer Sequence Length
M13 forward (-20F) 5'- TGT AAA ACG ACG GCC AGT - 3' 18
M13 reverse (-29R) 5' - CAG GAA ACA GCT ATG AC - 3' 17
T7 5' - TAA TAC GAC TCA CTA TAG GG - 3' 20
T7t 5' - GCT AGT TAT TGC TCA GCG G - 3' 19
Sp6 5' - CGA TTT AGG TGA CAC TAT AG - 3' 20
T3 5' - ATT AAC CCT CAC TAA AGG GA - 3' 20
pGEX 5' 5' - GGG CTG GCA AGC CAC GTT TGG TG - 3' 23
pGEX 3' 5' - CCG GGA GCT GCA TGT GTC AGA GG - 3' 23
-40F 5' - GTT TTC CCA GTC ACG AC - 3' 17
-47F 5' - CGC CAG GGT TTT CCC AGT CAC GAC - 3' 24
-48R (30nt_M13R)
5' - AGC GGA TAA CAA TTT CAC ACA GGA - 3' 24
-55R 5' - AAT TGT GAG CGG ATA ACA ATT TCA - 3' 24
BGH reverse 5' - TAG AAG GCA CAG TCG AGG - 3' 18
CMV forward 5' - CGC AAA TGG GCG GTA GGC GTG - 3' 21