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The Department's buildings are currently open for wet laboratory work only. We have carried out a comprehensive COVID-19 risk assessment process and have introduced a number of new measures to ensure the safety of our staff, including reduced building occupancy, strict social distancing, 'family'-based working, and increased cleaning and hygiene regimes. All staff who can work remotely will do so for the foreseeable future. Please continue to contact us by email until further notice.

Department of Biochemistry

 

The Department has conducted a comprehensive COVID-19 risk assessment process and has introduced a number of new measures to ensure the safety of our staff. In order to accommodate these new measures, our facilities will be operating revised services for the foreseeable future. Details of current arrangements and available services can be found here.

The NextSeq 500 Sequencing System delivers the power of high-throughput sequencing with the speed, simplicity, and affordability of a desktop NGS (next-generation sequencing) system. The fast, integrated, sample-to-results workflow enables many sequencing applications—including exomes, whole genomes, and transcriptomes—in a single run.

NextSeq500 Performance
Flow Cell Read Length (bp) Output (Gb) Run Time
High Output

300 cycle kit

(2 x 150)

100 - 120 29 hrs

150 cycle kit

(2 x 75)

50 -60 18 hrs

75 cycle kit

(1 x 75)

25 - 30 11 hrs
Mid Output

300 cycle kit

(2 x 150)

32 - 39 26 hrs

150 cycle kit

(2 x 75)

16 - 19

15 hrs

Flow Cell Single Reads Paired-End Reads
High Output up to 400 million up to 800 million
Mid Output up to 130 million up to 260 million

Data quality >75% higher than Q30 at 2 x 150 bp