The NextSeq 500 Sequencing System delivers the power of high-throughput sequencing with the speed, simplicity, and affordability of a desktop NGS (next-generation sequencing) system. The fast, integrated, sample-to-results workflow enables many sequencing applications—including exomes, whole genomes, and transcriptomes—in a single run.
| NextSeq500 Performance | |||
|---|---|---|---|
| Flow Cell | Read Length (bp) | Output (Gb) | Run Time |
| High Output |
300 cycle kit (2 x 150) |
100 - 120 | 29 hrs |
|
150 cycle kit (2 x 75) |
50 -60 | 18 hrs | |
|
75 cycle kit (1 x 75) |
25 - 30 | 11 hrs | |
| Mid Output |
300 cycle kit (2 x 150) |
32 - 39 | 26 hrs |
|
150 cycle kit (2 x 75) |
16 - 19 |
15 hrs |
|
| Flow Cell | Single Reads | Paired-End Reads |
|---|---|---|
| High Output | up to 400 million | up to 800 million |
| Mid Output | up to 130 million | up to 260 million |
Data quality >75% higher than Q30 at 2 x 150 bp