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The University has moved into its "red" phase in response to the coronavirus (COVID-19) outbreak. All University staff, except those needed for business-critical activity, are now working remotely. Please contact us by email until further notice.

Department of Biochemistry

 

The University has moved into its "red" phase in response to the coronavirus (COVID-19) outbreak. All University staff, except those needed for business-critical activity, are now working remotely. Our facilities will therefore not be able to provide a normal service for the foreseeable future. Details of current arrangements and available services can be found here.

The MiSeq supports a wide range of applications, including the following:

  • Targeted resequencing
  • TruSeq Custom Amplicon Sequencing
  • De novo sequencing (< 20Mb for ∼ 50x coverage, < 50Mb for ∼ 20x coverage)
  • Small RNA sequencing
  • Metagenomics
  • Library QC

The NextSeq500 supports a wide range of applications, including the following:

  • Whole genome sequencing
  • Exome sequencing
  • Transcriptome sequencing
  • Targeted resequencing
Application MiSeq NextSeq500
DNA Sequencing
Whole Genome - Large (e.g. Human)1 1 1-10
Whole Genome - Small (e.g. E. coli)2 1-96 120-792
Targeted - Exome or large panels3 1 1-12
Targeted - Small gene panels4 3 15-48
RNA Sequencing
RNA Profilinga 1-2 12-36
Transcriptome Analysisb - 3-10
Small RNA Analysisc 1-5 25-80
Targeted RNAd 384 384
Regulation Applications
ChIP-Seqi 1 8-24
Methylation Analysisii - 1

DNA sequencing applications based on:

  1. 30x coverage of a human genome
  2. 30x or greater coverage of a 5 Mb microbial genome
  3. 4 Gb per sample at 2x75bp read length
  4. TruSight One Sequencing Panel

RNA sequencing applications based on:

  1. 10 million reads per sample
  2. 40 million reads per sample
  3. 5 million reads per sample
  4. based on TruSeq indexing and up to 65 targets for MiSeq, 1041 targets for NextSeq

Regulation sequencing applications based on:

  1. 15 million reads per sample for identification of transcription factor binding
  2. 30x coverage