Information courtesy of Illumina
The Illumina MiSeq System combines proven sequencing by synthesis (SBS) technology with a workflow that enables you to go from DNA to analyzed data in as little as eight hours. The MiSeq integrates cluster amplification, sequencing and data analysis in a single instrument.
| MiSeq Performance | |||
|---|---|---|---|
| Total time | Output | ||
| Read Length | Current | Current | |
| 50 cycle kit (2 x 25 bp) V2 | 5.5 hrs | 750 - 850 Mb | |
| 150 cycle kit (2 x 75 bp) V3 | 20 hrs | 3.3 - 3.8 Gb | |
| 300 cycle kit (2 x 150 bp) v2 Nano | 300 Mb | ||
| 300 cycle kit (2 x 150 bp) v2 Micro | 1.2 Gb | ||
| 300 cycle kit (2 x 150 bp) V2 | 24 hrs | 4.5 - 5.1 Gb | |
| 500 cycle kit (2 x 250 bp) V2 Nano | 500 Mb | ||
| 500 cycle kit (2 x 250 bp) V2 | 39 hrs | 7.5 - 8.5 Gb | |
| 600 cycle kit (2 x 300 bp) V3 | 55 hrs | 13.2 - 15 Gb | |
| Reads Passing Filter | V2 Chemistry | V3 Chemistry | |
| Single Reads |
15 - 17 million Micro 4 million Nano 1 million |
22 - 25 million | |
| Paired-End Reads |
30 - 34 million Micro 8 million Nano 2 million |
44 - 50 million | |
| Quality Scores | |||
| 1 x 36 bp | > 90% bases higher than Q30 | ||
| 2 x 25 bp | > 90% bases higher than Q30 | ||
| 2 x 75 bp | > 85% bases higher than Q30 | ||
| 2 x 150 bp | > 80% bases higher than Q30 | ||
| 2 x 250 bp | > 75% bases higher than Q30 | ||
| 2 x 300 bp | > 70% bases higher than Q30 | ||