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The Department's buildings are currently open for wet laboratory work only. We have carried out a comprehensive COVID-19 risk assessment process and have introduced a number of new measures to ensure the safety of our staff, including reduced building occupancy, strict social distancing, 'family'-based working, and increased cleaning and hygiene regimes. All staff who can work remotely will do so for the foreseeable future. Please continue to contact us by email until further notice.

Department of Biochemistry

 

The Department has conducted a comprehensive COVID-19 risk assessment process and has introduced a number of new measures to ensure the safety of our staff. In order to accommodate these new measures, our facilities will be operating revised services for the foreseeable future. Details of current arrangements and available services can be found here.

Information courtesy of Illumina

The Illumina MiSeq System combines proven sequencing by synthesis (SBS) technology with a workflow that enables you to go from DNA to analyzed data in as little as eight hours. The MiSeq integrates cluster amplification, sequencing and data analysis in a single instrument.

MiSeq Performance
Total time Output
Read Length Current Current
50 cycle kit (2 x 25 bp) V2 5.5 hrs 750 - 850 Mb
150 cycle kit (2 x 75 bp) V3 20 hrs 3.3 - 3.8 Gb
300 cycle kit (2 x 150 bp) v2 Nano 300 Mb
300 cycle kit (2 x 150 bp) v2 Micro 1.2 Gb
300 cycle kit (2 x 150 bp) V2 24 hrs 4.5 - 5.1 Gb
500 cycle kit (2 x 250 bp) V2 Nano 500 Mb
500 cycle kit (2 x 250 bp) V2 39 hrs 7.5 - 8.5 Gb
600 cycle kit (2 x 300 bp) V3 55 hrs 13.2 - 15 Gb
Reads Passing Filter V2 Chemistry V3 Chemistry
Single Reads

15 - 17 million

Micro 4 million

Nano 1 million

22 - 25 million
Paired-End Reads

30 - 34 million

Micro 8 million

Nano 2 million

44 - 50 million
Quality Scores
1 x 36 bp > 90% bases higher than Q30
2 x 25 bp > 90% bases higher than Q30
2 x 75 bp > 85% bases higher than Q30
2 x 150 bp > 80% bases higher than Q30
2 x 250 bp > 75% bases higher than Q30
2 x 300 bp > 70% bases higher than Q30