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The University has moved into its "red" phase in response to the coronavirus (COVID-19) outbreak. All University staff, except those needed for business-critical activity, are now working remotely. Please contact us by email until further notice.

Department of Biochemistry

 

The University has moved into its "red" phase in response to the coronavirus (COVID-19) outbreak. All University staff, except those needed for business-critical activity, are now working remotely. Our facilities will therefore not be able to provide a normal service for the foreseeable future. Details of current arrangements and available services can be found here.

Information courtesy of Illumina

The Illumina MiSeq System combines proven sequencing by synthesis (SBS) technology with a workflow that enables you to go from DNA to analyzed data in as little as eight hours. The MiSeq integrates cluster amplification, sequencing and data analysis in a single instrument.

MiSeq Performance
Total time Output
Read Length Current Current
50 cycle kit (2 x 25 bp) V2 5.5 hrs 750 - 850 Mb
150 cycle kit (2 x 75 bp) V3 20 hrs 3.3 - 3.8 Gb
300 cycle kit (2 x 150 bp) v2 Nano 300 Mb
300 cycle kit (2 x 150 bp) v2 Micro 1.2 Gb
300 cycle kit (2 x 150 bp) V2 24 hrs 4.5 - 5.1 Gb
500 cycle kit (2 x 250 bp) V2 Nano 500 Mb
500 cycle kit (2 x 250 bp) V2 39 hrs 7.5 - 8.5 Gb
600 cycle kit (2 x 300 bp) V3 55 hrs 13.2 - 15 Gb
Reads Passing Filter V2 Chemistry V3 Chemistry
Single Reads

15 - 17 million

Micro 4 million

Nano 1 million

22 - 25 million
Paired-End Reads

30 - 34 million

Micro 8 million

Nano 2 million

44 - 50 million
Quality Scores
1 x 36 bp > 90% bases higher than Q30
2 x 25 bp > 90% bases higher than Q30
2 x 75 bp > 85% bases higher than Q30
2 x 150 bp > 80% bases higher than Q30
2 x 250 bp > 75% bases higher than Q30
2 x 300 bp > 70% bases higher than Q30