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Department of Biochemistry

Sanger Instrument

Fragment analysis applications are those in which fluorescent fragments of DNA (produced by PCR using primers designed for a specific experiment) are separated using capillary electrophoresis and sized by comparison to a size standard.

Most fragment analysis applications have simplified workflows—straightforward sample preparation (without the purification required for sequencing samples), ability to multiplex, and applications can all be separated using the same capillary electrophoresis polymer.

Fragment Analysis Trace

ABI 3130XL instrument
What can I do with fragment analysis?

  • Microsatellite (STR) analysis
  • SNP Genotyping
  • Fingerprinting
  • Relative Fluorescence Quantitation

Available Dye SetsAvailable Dye Sets

The DNA Sequencing Facility offers Fragment Analysis runs on the ABI 3130XL, a 16-capillary instrument with a 36cm array.

Please for further details.