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The Department's buildings are currently open for wet laboratory work only. We have carried out a comprehensive COVID-19 risk assessment process and have introduced a number of new measures to ensure the safety of our staff, including reduced building occupancy, strict social distancing, 'family'-based working, and increased cleaning and hygiene regimes. All staff who can work remotely will do so for the foreseeable future. Please continue to contact us by email until further notice.

Department of Biochemistry

 

The Department has conducted a comprehensive COVID-19 risk assessment process and has introduced a number of new measures to ensure the safety of our staff. In order to accommodate these new measures, our facilities will be operating revised services for the foreseeable future. Details of current arrangements and available services can be found here.

Sanger Instrument

Fragment analysis applications are those in which fluorescent fragments of DNA (produced by PCR using primers designed for a specific experiment) are separated using capillary electrophoresis and sized by comparison to a size standard.

Most fragment analysis applications have simplified workflows—straightforward sample preparation (without the purification required for sequencing samples), ability to multiplex, and applications can all be separated using the same capillary electrophoresis polymer.

Fragment Analysis Trace

ABI 3130XL instrument

What can I do with fragment analysis?

  • Microsatellite (STR) analysis
  • SNP Genotyping
  • Fingerprinting
  • Relative Fluorescence Quantitation

 

Available Dye SetsAvailable Dye Sets

The DNA Sequencing Facility offers Fragment Analysis runs on the ABI 3130XL, a 16-capillary instrument with a 36cm array.

Please for further details.