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The Department's buildings are currently open for wet laboratory work only. We have carried out a comprehensive COVID-19 risk assessment process and have introduced a number of new measures to ensure the safety of our staff, including reduced building occupancy, strict social distancing, 'family'-based working, and increased cleaning and hygiene regimes. All staff who can work remotely will do so for the foreseeable future. Please continue to contact us by email until further notice.

Department of Biochemistry

 

The Department has conducted a comprehensive COVID-19 risk assessment process and has introduced a number of new measures to ensure the safety of our staff. In order to accommodate these new measures, our facilities will be operating revised services for the foreseeable future. Details of current arrangements and available services can be found here.

DNA Sequencing Team 2019

The DNA sequencing facility in the Biochemistry Department was started in 1993 under the direction of Professor Peter Leadlay. Since that time the Facility has developed rapidly as the ability to sequence genes has become a pervasive and essential tool in all areas of biological research. The scale of the projects tackled now ranges from the validation of single mutations in individual genes up to sequencing of entire microbial genomes. We have continually updated the equipment, in order to maximise the information obtained and to minimise turn-around time, and to make the Facility as user-friendly as possible.

Sanger DNA sequencing is carried out on an Applied Biosystems 3730xl DNA Analyser, which utilises a 96 capillary array and on which 96 reactions may be run in approximately two and a half hours. Each reaction potentially gives up to 800 nucleotides of sequence from high quality templates.

The Next-Gen Sequencing service has three Illumina MiSeqs and an Illumina NextSeq500. The MiSeq sequencer was purchased to offer Illumina's high throughput, short read technology to our customers. The NextSeq500, now offers larger throughput and very competitive prices.

Facility staff have expertise in most aspects of DNA sequence analysis and can offer help and advice to both experienced researchers and to beginners. We welcome proposals for large-scale projects as well as for more routine small-scale sequencing. Our Facility offers sequencing services to anyone, we are not limited to only Departmental or University users.  

Contact us

Facility opening hours

Monday - Friday from 6am - 6pm

If you need to submit samples during the times when the Biochemistry reception is closed then you may leave the samples attached to the request form in the wire basket in reception, or call the lab.

 

- Facility Manager

Tel: 01223 (3)33660

Tel: 01223 (7)66311

Room 2.84 in the Biochemistry Sanger Building, 80 Tennis Court Road, Cambridge, CB2 1GA

- Next-Generation Sequencing

Tel: 01223 (3)33657