 |
Contact Mike Weldon
01223 333638
m.a.weldon@bioc.cam.ac.uk |
Protein Sequence Analysis (Edman degradation)
Status of this Service |
Normal |
Orders are accepted from any Cambridge University Department. Other Academic Institutions may be accommodated if there is spare capacity. New customers should discuss their requirements with Mike Weldon (333638). Results typically available in 1-3 days (holidays excepted).
- Please read guidance before preparing your sample
- All orders must be on an official order form, detailing all relevant and requested information
- Attach a hard copy departmental purchase order (Biochemistry only - a grant code)
- Postal address
Edman sequence analysis prices
 PVDF-blotted samples - prices as below Solution samples - add £10 per sample to cover preparation
Please choose from the following fixed options:
- 5 residues of sequence (recommended minimum for comparison to known sequence)
- Biochemistry Department - £75
- Cambridge University Departments - £90
- External Academic Clients - £120+VAT (no exemption); see important notes
- Industry (where capacity permits) - £210+VAT (no exemption); see important notes
- 10 residues of sequence (recommended minimum for database searches)
- Biochemistry Department - £150
- Cambridge University Departments - £180
- External Academic Clients - £240+VAT (no exemption); see important notes
- Industry - £420+VAT (no exemption); see important notes
- Charges per residue attempted beyond 10
- Biochemistry Department - £12
- Cambridge University Departments - £15
- External Academic Clients - £20+VAT (no exemption); see important notes
- Industry - £35+VAT (no exemption); see important notes
Sample preparation
Purity is of paramount importance. Investigators preparing samples in solution should provide evidence of purity. Samples can be accepted in moderate concentrations of salts (0.1M) as samples will generally be immobilised on a PVDF membrane using a filter device (ProSorb from Perkin Elmer) which allows buffers to be washed out. Detergents should be avoided as they reduce binding capacity. Samples prepared by electroblotting from gels - please supply the whole blot and accompany it with a photocopy to mark the bands of interest. You must use PVDF for blotting, not nitrocellulose. Individual sheets of PVDF can be obtained from the Facility at cost (£10 for 20x20cm). Any known sequence information should also be supplied to assist in setting instrument parameters and enabling database searches.
Sample amount - 10pmol or more required on the blot. See example of band amounts at different masses.
Revisions: form 17 May 04, prices Jan 2003, Jun 2005, Oct 2010 *Excluding VAT. Prices are continually reviewed and subject to change without notice.
Notes
You must agree to Standard Terms for Processing Samples on order form. VAT will be payable on all external orders as exemption (zero-rate) does not cover supply of 'information' (i.e. results), even if VAT exemption is claimed by the customer. Sorry, but that's VAT law. No VAT is payable by University of Cambridge customers.
Charges will be levied as listed even if an identification is unsuccessful as significant work is involved with each sample. If no result is forthcoming through operator error or instrument failure, the charge will be waived.
VAT legislation Once inside the scope of VAT, the VAT law is such that unless something is specifically 'exempted' or 'zero-rated' it will be standard rate. The specific bit of legislation allowing zero-rating (also referred to as medical exemption) for substances provided is in the VAT Act 1994, Sch 8, Item No 10: "The supply to a charity of a substance directly used for synthesis or testing in the course of medical or vetinary research". This does not cover information supplied as results based on analysis of a substance. VAT zero-rate can only apply where PNAC Facility is actually supplying a substance such as peptides. |
