Curriculum vitae: Dr Len C. Packman

University Academic Record

• 1972-1975: University of Leicester B.Sc. (Hons) degree in Biological Sciences, Class I.
• 1975-1978: University of Leicester, Department of Biochemistry Ph.D. in Biochemistry under the supervision of Prof. W.V. Shaw
• 1979-1986: University of Cambridge, Department of Biochemistry Post-doctoral Research Associate in the laboratory of Prof. R.N. Perham.
• 1986-1989: Senior Research Associate in the same laboratory and responsible for the University of Cambridge Protein Sequencing Facility.
• 1989-present: Assistant Director of Researchin the Department of Biochemistry, responsible for the Protein and Nucleic Acid Chemistry Facility

General Interests
Performing in amateur Operetta & Musicals , music, and film/TV science fiction...

Research Interests & Publications

Microanalytical techniques applied to protein and nucleic acid characterisation. I've worked on lots of topics ranging from multienzyme complexes to toxins to peptide synthesis. See the titles of the papers for more information.

PUBLICATIONS - Choose a year

1. Fitton, J.E., Packman, L.C., Harford, S., Zaidenzaig, Y. & Shaw, W.V. (1978). Plasmids and the evolution of chloramphenicol resistance. In Microbiology-1978 (Schlessinger, D. ed.) pp. 249-252. Amer. Soc. Microbiol. Washington D.C.

2. Packman, L.C. & Smith, D.J.H. (1978). A simplified route to [¹⁴C] dimethyl suberimidate for polypeptide crosslinking studies. FEBS Lett. 91, 178-181.

3. Zaidenzaig, Y., Fitton, J.E., Packman, L.C. & Shaw, W.V. (1979). Characterisation and comparison of chloramphenicol acetyltransferase variants. Eur. J. Biochem. 100, 609-618.

4. Shaw, W.V., Packman, L.C., Burleigh, B.D., Dell, A., Morris, H.R. & Hartley, B.S. (1979). Primary structure of a chloramphenicol acetyltransferase specified by R-plasmids. Nature 282, 870-872.

5. Packman, L.C. & Shaw, W.V. (1981). Identification of 'buried' lysine residues in two variants of chloramphenicol acetyltransferase specified by R-factors. Biochem. J. 193,
525-539.

6. Packman, L.C. & Shaw, W.V. (1981). The use of naturally occurring hybrid variants of chloramphenicol acetyltransferase to investigate subunit contacts. Biochem. J. 193,
541-552.

7. Stanley, C.J., Packman, L.C., Danson, M.J., Henderson, C.E. & Perham, R.N. (1981). Intramolecular coupling of active sites in the pyruvate dehydrogenase multienzyme complexes from bacterial and mammalian sources. Biochem.J. 195, 715-721.

8. Packman, L.C. & Perham, R.N. (1982). An amino acid sequence in the active site of lipoamide dehydrogenase from Bacillus stearothermophilus. FEBS Lett. 139, 155-158.

9. Packman, L.C., Perham, R.N. & Roberts, G.C.K. (1982). Crosslinking and 1H-n.m.r. spectroscopy of the pyruvate dehydrogenase complex of Escherichia coli. Biochem. J. 205, 389-396.

10. Packman, L.C. & Perham, R.N. (1982). Quaternary structure of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus studied by a new reversible cross-linking procedure with bis(imidoesters). Biochemistry 21, 5171-5175.

11. Roberts, G.C.K., Duckworth, H.W., Packman, L.C. & Perham, R.N. (1983). Mobility and active-site coupling in 2-oxo acid dehydrogenase complexes. In Mobility and Function in Proteins and Nucleic Acids. Ciba Foundation Symposium 93 (eds R. Porter, M. O'Connor & J. Whelan) pp.47-62. Pitman Books, London.

12. Packman, L.C., Stanley, C.J. & Perham, R.N. (1983). Temperature-dependence of intramolecular coupling of active sites in pyruvate dehydrogenase multienzyme complexes. Biochem. J. 213, 331-338.

13. Packman, L.C., Perham, R.N. & Roberts, G.C.K. (1984). Domain structure and 1H-n.m.r. spectroscopy of the pyruvate dehydrogenase complex of Bacillus stearothermophilus. Biochem. J. 217, 219-227.

14. Packman, L.C., Hale, G. & Perham, R.N. (1984). Repeating functional domains in the pyruvate dehydrogenase multienzyme complex of Escherichia coli. EMBO J. 3, 1315-1319.

15. Guest, J.R., Lewis, H.M., Graham, L.D., Packman, L.C. & Perham, R.N. (1985). Genetic reconstruction and functional analysis of the repeating lipoyl domains in the pyruvate dehydrogenase multienzyme complex of Eschericha coli. J. Mol. Biol. 185,
743-754.

16. Graham, L.D., Guest, J.R., Lewis, H.M., Miles, J.S., Packman, L.C., Perham, R.N. & Radford, S.E. (1986). The pyruvate dehydrogenase multi-enzyme complex of Escherichia coli: genetic reconstruction and functional analysis of the lipoyl domains. Phil. Trans. R. Soc. Lond. A 317, 391-404.

17. Packman, L.C. & Perham, R.N. (1986). Chain folding in the dihydrolipoyl acyltransferase components of the 2-oxo acid dehydrogenase complexes from Escherichia coli. Identification of a segment involved in binding the E3 subunit. FEBS Lett. 206, 193-198.

18. Packman, L.C. & Perham, R.N. (1987). Limited proteolysis and sequence analysis of the 2-oxo acid dehydrogenase complexes from Escherichia coli. Cleavage sites and domains in the dihydrolipoyl acyltransferase components. Biochem. J. 242, 531-538.

19. Perham, R.N., Packman, L.C. & Radford, S.E. (1987). 2-Oxo acid dehydrogenase multienzyme complexes: in the beginning and halfway there. In Krebs' Citric Acid Cycle - Half a Century and Still Turning. Biochemical Society Symposium Vol. 54 (eds J. Kay & P.D.J. Weitzman) pp.67-81.

20. Packman, L.C., Borges, A. & Perham, R.N. (1988). Amino acid sequence analysis of the lipoyl and peripheral subunit-binding domains in the lipoate acetyltransferase component of the pyruvate dehydrogenase complex from Bacillus stearothermophilus. Biochem. J. 252, 79-86.

21. Dunn, P.P.J., Packman, L.C., Pappin, D. & Gray, J.C. (1988). N-terminal amino acid sequence analysis of the subunits of pea photosystem I. FEBS Lett. 228, 157-161.

22. Miller, A.D., Hart, G.J., Packman, L.C. & Battersby, A.R. (1988). Evidence that the pyrromethane cofactor of hydroxymethylbilane synthase (porphobilinogen deaminase) is bound to the protein through the sulphur atom of cysteine-242. Biochem. J. 254, 915-918.

23. Webber, A.N., Packman, L.C., Chapman, D.J., Barber, J. & Gray, J.C. (1989). A fifth chloroplast-encoded polypeptide is present in the photosystem II reaction centre complex. FEBS Lett. 242, 259-262.

24. Webber, A.N., Packman, L.C. & Gray, J.C. (1989). A 10kDa polypeptide associated with the oxygen-evolving complex of photosystem II has a putative C-terminal non-cleavable thylakoid transfer domain. FEBS Lett. 242, 435-438.

25. Webber, A.N., Hird, S.M., Packman, L.C., Dyer, T.A. & Gray, J.C. (1989). A photosystem II polypeptide is encoded by an open reading frame co-transcribed with the genes for cytochrome b-559 in wheat chloroplast DNA. Plant Mol. Biol. 12, 141-151.

26. Graham, L.D., Packman, L.C. & Perham, R.N. (1989). Kinetics and specificity of reductive acylation of lipoyl domains from 2-oxo acid dehydrogenase multienzyme complexes. Biochemistry 28, 1574-1581.

27. Packman, L.C. & Perham, R.N. (1989). The domain structures of the E2 components of the pyruvate dehydrogenase multienzyme complex from Bacillus stearothermophilus and Saccharomyces cererisiae. In Methods in Protein Sequence Analysis (ed. B. Wittman-Liebold) pp.440-449.Springer Verlag, Berlin.

28. Miller, A.D., Packman, L.C., Hart, G.J., Alefounder, P.R., Abell, C. & Battersby, A.R. (1989). Evidence that pyridoxal phosphate modification of lysine residues (Lys-55 and Lys-59) causes inactivation of hydroxymethylbilane synthase (porphobilinogen deaminase). Biochem. J. 262, 119-124.

29. Robb, S., Packman, L.C. & Evans, P.D. (1989). Isolation, primary structure and bioactivity of schistoFLRF-amide, a FMRF-amide-like neuropeptide from the locust Schistocerca gregaria. Biochem. Biophys. Res. Commun. 160, 850-856.

30. Perham, R.N. & Packman, L.C. (1989). 2-oxo acid dehydrogenase multienzyme complexes: domains, dynamics and design. In: Alpha-Keto Acid Dehydrogenase Complexes: Organization, Regulation and Biomedical Ramifications (eds T.E. Roche & M.S. Patel). Ann. N.Y. Acad. Sci. 573, 1-20.

31. Hill, C.S., Packman, L.C. & Thomas, J.O. (1990). Phosphorylation at clustered -Ser-Pro-X-Lys/Arg- motifs in sperm-specific histones H1 and H2B. EMBO J. 9, 805-813

32. Dardel, F., Packman, L.C. & Perham, R.N. (1990). Expression in Escherichia coli of a sub-gene encoding the lipoyl domain of the pyruvate dehydrogenase complex of
Bacillus stearothermophilus. FEBS Lett. 264, 206-210.

33. Packman, L.C. (1990) Identification of phosphoserine in protein sequence analysis. J. Protein Chemistry 9, 307-308

34. Borges, A., Hawkins, C.F., Packman, L.C. & Perham, R.N. (1990). Cloning and sequence analysis of the genes encoding the dihydrolipoyl acetyltransferase and dihydrolipoamide dehydrogenase components of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus. Eur.J.Biochem. 194, 95-102

35. Perham, R.N., Dardel, F., Laue, E.D. & Packman, L.C. (1991) The structure and post-translational modification of lipoyl domains in 2-oxo acid dehydrogenase multienzyme complexes. In Methods in Protein Sequence Analysis (eds. H. Jornvall, J.-O. Hoog & A.-M. Gustavsson) pp. 353-362, Birkhauser Verlag, Basel; Boston; Berlin

36. Nutkins, J.C., Mortishire-Smith, R.J., Packman, L.C., Brodey, C.L., Rainey, P.B., Johnstone, K. & Williams D.H. (1991) Structure determination of Tolaasin, an extracellular lipodepsipeptide produced by the mushroom pathogen, Pseudomonas tolasii Paine. J.Amer. Chem. Soc. 113, 2621-2627

37. Caffrey, P., Green, B., Packman, L.C., Rawlings, B.J., Staunton, J. and Leadlay, P.F. (1991) An acyl-carrier-protein - thioesterase domain from the 6-deoxyerythronolide B synthase of Saccharopolyspora erythraea - High-level production, purification and characterisation in Escherichia coli. Eur. J. Biochem. 195, 823-830

38. Mortishire-Smith, R.J., Nutkins, J.C., Packman, L.C., Brodey, C.L., Rainey, P.B., Johnstone, K. & Williams, D.H. (1991) Determination of the structure of an extracellular peptide produced by the mushroom saprotroph Pseudomonas reactans. Tetrahedron 47, 3645-3654

39. Packman, L.C., Green, B. & Perham R.N. (1991) Lipoylation of the E2 components of the 2-oxo acid dehydrogenase multienzyme complexes from Eschericia coli. Biochem. J. 277, 153-158

40. Xia, M-Q., Tone, M., Packman, L., Hale, G. & Waldmann, H. (1991) Characterization of the CAMPATH-1 (CDw52) antigen: biochemical analysis and cDNA cloning reveal an unusually small peptide backbone. Eur. J. Immunol. 21, 1677-1684

41. Gay, N.J., Packman, L.C., Weldon, M.A. & Barna, J.C.J. (1991) 'A leucine-rich repeat peptide derived from the Drosophila Toll receptor forms extended filaments with ß-sheet structure' FEBS Letters 291, 87-91

42. Packman, L.C. & Hipps, D.S. (1991) The structural domains in the E2 component of the pyruvate dehydrogenase multienzyme complex from Bacillus stearothermophilus. Biochem. Soc. Trans.19, 917-922

43. Boyd, G., Mathews, F.S., Packman, L.C. & Scrutton, N.S. (1992) Trimethylamine dehydrogenase of bacterium W3A1. Molecular cloning, sequence determination and over-expression of the gene FEBS Letters, 308, 271-276

44. Weldon, M & Packman, L.C. (1992) 'Disulphide bridge formation in synthetic peptides - rapid monitoring of the process by capillary electrophoresis' in Innovation and Perspectives in Solid Phase Synthesis 1992, ed R. Epton, SPCC UK Ltd. pp 489-493

45. Perham, R.N., Borges, A., Dardel, F., Graham, L.D., Hawkins, C.F., Laue, E.D. & Packman, L.C. (1992) 'The lipoyl domain and its role in thiamin diphosphate-dependent oxidative decarboxylation' in Proceedings of the 1st International Congress on Vitamins and Biofactors in Life Science. Journal of Nutritional Science and Vitaminology. Special Issue, 1992. Ed Tadashi, Kobyashi . Center for Academic Publications, Japan, pp 457-460

46. Xia, M-Q, Hale, G., Lifely, M.R., Ferguson, M.A.J., Campbell, D., Packman, L.C. & Waldmann, H. (1993) 'Structure of the CAMPATH-1 antigen, a GPI-anchored glycoprotein which is an exceptionally good target for complement lysis' Biochem. J. 293, 633-640

47. Cox, P.L., Evans, P.A., Packman, L.C., Williams, D.H. & Woolfson, D.N. (1993) 'Dissecting the structure of a partially folded protein: CD and NMR studies of peptides from ubiquitin' J. Mol. Biol. 234, 483-492

48. Hipps, D.S., Packman, L.C., Allen, M.D., Fuller, C., Sakaguchi, K., Appella, E. and Perham, R.N. (1994) The peripheral subunit-binding domain of the dihydrolipoyl acetyltransferase component of the pyruvate dehydrogenase complex of Bacillus stearothermophilus: preparation and characterization of its binding to the dihydrolipoyl dehydrogenase component. Biochem. J. 297, 137-143

49. Packman, L.C. (1994) 'Protein chemical methods for molecular biologists' Methods Mol. Cell. Biol. 4, 189-198

50. Scrutton, N.S., Packman, L C., Mathews, F.S., Rohlfs, R.J. & Hille, R. (1994) Assay of redox centres in trimethylamine dehydrogenase of bacterium W3A1. Properties of the wild type enzyme and a C30A mutant expressed from a cloned gene in Escherichia coli J. Biol. Chem. 269, 13942-13950

51. Packman, L.C., Quibell, M. & Johnson, T (1994) The roles of electrospray mass spectrometry, counterion distribution monitoring and N-(2-hydroxy-4-methoxybenzyl) (Hmb) backbone protection in the successful synthesis of a difficult peptide sequence. Peptide Research 7, 125-131

52. Gray, J.C., Rochford, R.J. & Packman, L.C. (1994) Proteolytic removal of the C-terminal transmembrane region of cytochrome f during extraction from turnip and charlock leaves generates a water-soluble monomeric form of the protein. Eur. J. Biochem. 223, 481-488

53. Hutchinson, I., Weldon, M., Packman, L.C., Quibell, M. & Johnson, T (1994) Disrupting secondary structure during peptide synthesis via backbone amide protection: application to the synthesis of an HIV integrase fragment in Innovation and Perspectives in Solid Phase Synthesis 1993, Ed. R. Epton, SPCC UK Ltd. 547-550

54. Quibell, M., Owen, D., Packman, L.C. & Johnson, T (1994) Suppression of piperidine-mediated side-product formation for Asp(OBut)-containing peptides by use of N-(2-hydroxy-4-methoxybenzyl) (Hmb) backbone amide protection. J. Chem. Soc. Chem. Commun.1994, 2343-2344

55. Stanley, P., Packman, L.C., Koronakis, V. & Hughes, C (1994) Fatty acylation of two internal lysine residues required for the toxic activity of E. coli hemolysin Science 266, 1992-1996

56. Scrutton, N.S., Packman, L.C. & Mathews, F.S. Flavinylation of trimethylamine dehydrogenase of bacterium W3A1 expressed in Escherichia coli in Flavins and Flavoproteins 1994, Ed. Yagi, K, Walter de Gruyter & Co, Berlin, NY pp 803-806

57. Packman, L.C. & Berry, A. (1995) A reactive, surface cysteine residue of the Class II fructose-1,6-bisphosphate aldolase of Escherichia coli revealed by electrospray mass spectrometry. Eur. J. Biochem. 277, 510-515.

58. Packman, L.C., Mewies, M. and Scrutton, N.S. (1995) The flavinylation reaction of trimethylamine dehydrogenase: analysis by directed mutagenesis and electrospray mass spectrometry. J. Biol. Chem. 270, 13186-13191

59. Wilson, E. K., Mathews, F. S., Packman, L. C. & Scrutton, N.S. (1995) Electron tunnelling in substrate reduced trimethylamine dehydrogenase: kinetics of electron transfer and analysis of the tunneling pathway. Biochemistry 34, 2584-2591

60. Packman, L.C., Webster, C & Gray, J. (1995) The use of MALDITOF mass spectrometry and amino acid sequence analysis in characterising small amounts of N-blocked protein in Methods in Protein Structure Analysis, Plenum Press, eds M.Z. Atassi, E. Apella pp. 175-183

61. Mewies, M., Packman, L.C. & Scrutton, N.S. (1995) Dissection of the FMN binding site in trimethylamine dehydrogenase. Biochem Soc. Trans. 23, 509S

62. Packman, L.C. (1995) N-2-hydroxy-4-methoxybenzyl (Hmb) backbone protection strategy prevents double aspartimide formation in a 'difficult' peptide sequence. Tetrahedron Lett. 36, (41) 7523-7526

63. Quibell, M., Packman, L.C. & Johnson, T. (1995) Synthesis of the 3-repeat region of human Tau-2 by the solid phase assembly of backbone amide-protected segments J. Amer. Chem. Soc. 117, 11656-11668

64. Sumiya, M., Davis, E.O., Packman, L.C., McDonald, T.P. & Henderson, P.J.F. (1995) Molecular genetics of a receptor protein for D-xylose, encoded by the gene xyl F, in Escherichia coli.. Receptors and Channels, 3, 117-128

65. Yang, C-C., Packman, L.C. and Scrutton, N.S. (1995) The primary structure of Hyphomicrobium X dimethylamine dehydrogenase: relationship to trimethylamine dehydrogenase and implications for substrate recognition. Eur J. Biochem., 232, 264-271

66. Searle, M.S., Williams, D.H. & Packman, L.C. (1995) Folding of a short linear peptide into a water-stable ß-hairpin: non-native structure in a peptide derived from the N-terminal sequence of ubiquitin. Nature Struct. Biol. 2, 999-1006.

67. Raine, A.R.C., Yang, C-C., Packman, L.C., White, S.C., Mathews, F.S. & Scrutton, N.S. (1995) Protein recognition of ammonium cations using side-chain aromatics: a structural variation for secondary ammonium ligands. Protein Science, 4, (12), 2625-2628

68. Colfen, H., Harding, S.E., Wilson, E.K., Packman, L.C. Scrutton, N.S. (1996) Homodimeric and expanded behavior of trimethylamine dehydrogenase in solution at different temperatures. European Biophys. J., 24, (3), 159-164

69. Mewies, M., Packman, L.C., Mathews, F.S. & Scrutton, N.S. (1996) Flavinylation in wild-type trimethylamine dehydrogenase and differentially charged mutant enzymes: a study of the protein environment around the N1 of the flavin isoalloxazine. Biochem. J. 317, 267-272

70. Johnson, T., Packman, L.C., Hyde, C.B., Owen, D. & Quibell, M.(1996) Backbone protection and its application to the synthesis of a difficult phosphopeptide sequence. J. Chem.Soc.-Perkin Trans., 1, 1996, (7) 719-728

71. Quibell, M., Packman, L.C. and Johnson, T. (1996) Identification of coupling conditions proceeding with low C-terminal epimerization during the assembly of fully protected backbone-substituted peptide segments. J. Chem. Soc. Perkin Trans., 1, 1996, (11) 1219-1225

72. Quibell, M., Packman, L.C. and Johnson, T. (1996) Solid phase assembly of backbone amide-protected peptide segments: an efficient and reliable strategy for the synthesis of small proteins J. Chem. Soc. Perkin Trans., 1, 1996, (11) 1227-1234

73. Goytisolo, F.A., Packman, L.C. & Thomas, J.O. (1996) Photoaffinity labelling of a DNA binding site on the globular domain of histone H5. Eur. J. Biochem. 1996, 242, (3), 619-626

74. Packman, L.C., Kubota, K., Parker , J. & Gay, N.J. (1997) Casein kinase 11 phosphorylates Ser 468 in the PEST domain of the Drosophila IkB homologue cactus. (1997) FEBS Lett.400, (1), 45-50

75. Searle, M.S., Zerella, R., Williams, D.H., Packman, L.C. (1996) Native-like beta-hairpin structure in an isolated fragment from Ferredoxin - NMR and CD studies of solvent effects on the N-terminal 20 residues. Protein Engineering, 9, (7), 559-565

76. Ball, R.W. and Packman, L.C. (1997) MALDITOF mass spectrometry as a rapid quality control method in oligonucleotide synthesis. Anal. Biochem., 246, 185-194

77. Webster, C.I., Packman, L.C., Pwee, K-H. & Gray, J.C. (1997) High mobility group proteins HMG-1 and HMG-I/Y bind to a positive regulatory region of the pea plastocyanin gene promoter. The Plant Journal, 11, (1) 703-715

78. Scrutton, N.S., Wilson, E.K., Mewies, M., Packman, L.C., Mathews, F.S., Huang, L & Hille, R. (1997) Trimethylamine dehydrogenase: Mechanism and assembly of a complex iron-sulfur flavoprotein. Flavins and Flavoproteins 1996, (Eds. Stevenson, K.J., Massey, V. & Williams, C.H. Jr.) University of Calgary Press, pp857-864

79. Packman, L.C. (1997) Spots before your eyes - Identifying proteins in 2D gels by MALDITOF-ms and composition analysis. FASEB Journal, 11, 1546

80. Mewies, M., Basran, J., Packman ,L.C., Hille, R., & Scrutton, N.S. (1997) Involvement of a flavin iminoquinone methide in the formation of 6-hydroxyflavin mononucleotide in trimethylamine dehydrogenase: A rationale for the existence of 8 alpha-methyl and C6-linked covalent flavoproteins. Biochemistry, 36, 7162-7168

81. Santoni, V., Rouquie, D., Doumas, P., Mansion, M., Boutry, M., Degand, H., Dupree, P., Packman, L., Sherrier, J., Prime, T., Bauw, G., Posada, E., Rouze, P., Dehais, P., Sahnoun, I., Barlier, I. & Rossignol, M. (1998) Use of a proteome strategy for tagging proteins present at the plasma membrane. Plant Journal, 16, (5), 633-641

82. Zerella, R,. Evans, P.A., Ionides, J.M.C., Packman, L.C., Trotter, B.W., Mackay, J.P. and Williams, D.H. (1999) Autonomous folding of a peptide corresponding to the N-terminal beta-hairpin from ubiquitin. Protein Science, 8, 1320-1331

83. Webster, C.I., Cooper, M.A., Packman, L.C., Williams, D.H. & Gray, J.C. (2000) Kinetic analysis of high-mobility-group proteins HMG-1 and HMG-I/Y binding to cholesterol-tagged DNA on a supported lipid monolayer. Nucleic Acids Research, 28, 1618-1624 84. Prime, T.A., Sherrier, D.J., Mahon, P., Packman, L.C. & Dupree, P. (2000) A proteomic analysis of organelles from Arabidopsis thaliana. Electrophoresis, 21, (16) 3488-3499

85. Webster, C.I., Packman, L.C. & Gray, J.C. (2001) HMG-1 enhances HMG-I/Y binding to an A/T-rich enhancer element from the pea plastocyanin gene. Eur. J. Biochem. 268 (11): 3154-3162

86. Becker. W., Himpel, S., Czajkowska, H., Packman, L.C., & Joost, H.G. (2001) Identification of autophosphorylation sites and characterization of their effects in the dual-specificity protein kinase DYRK1A. Naunyn-Schmiedebergs Arch. Pharmacol. 363 (4): 247 Suppl. S

87. Manifava, M., Thuring, J.W.J.F., Lim, Z.Y., Packman, L., Holmes, A.B. & Ktistakis, N.T. (2001) Differential binding of traffic-related proteins to phosphatidic acid- or phosphatidylinositol (4,5)-bisphosphate-coupled affinity reagents. J. Biol. Chem. 276 (12): 8987-8994

88. Himpel,S., Panzer,P., Eirmbter,K., Czajkowska,H., Sayed,M., Packman,L.C., Blundell,T., Kentrup,H., Grotzinger,J., Joost,H-G. & Becker,W. (2001) Identification of the autophosphorylation sites and characterization of their effects in the protein kinase DYRK1A. Biochem. J. 359, 497-505

89. Arnott, D.P., Gawinowicz, M., Grant, R.A., Lane, W.S., Packman, L.C., Speicher, K. and Stone, K. (2002) Proteomics in mixtures: Study results of ABRF-PRG02. J.Biomolecular Techniques 13, 179-186

90. Arnott, D.P., Gawinowicz, M.A., Grant, R.A., Neubert, T.A., Packman, L.C., Speicher, K.D., Stone, K. & Turck, C.W. (2003) ABRF-PRG03: Phosphorylation Site Determination. Journal of Biomolecular Techniques, 14, 205-215

92. DeGraff, K., Hekerman, P., Spelten, O., Herrmann, A., Packman, L.C., Büssow, K., Müller-Newen, G. and Becker, W. (2004) Characterization of cyclin L2, a novel cyclin with an arginine/serine-rich domain. J. Biol. Chem. 279, (6), 4612-4624

93. Yu, L-G., Packman. L.C., Weldon, M, Hamlett, J and Rhodes, J.M. (2004) Protein phosphatase 2A, a negative regulator of the ERK signaling pathway, is activated by tyrosine phosphorylation of putative HLA Class II-associated protein I (PHAPI)/pp32 in response to the antiproliferative lectin, jacalin. J. Biol. Chem., 279, 41377-41383.

94. Stephens, E., Sugars, J., Maslen, S.L., Williams, D.H., Packman, L.C. and Ellar, D.J. (2004) The N-linked oligosaccharides of aminopeptidase N from Manduca sexta. Site localization and identification of novel N-glycan structures. European Journal of Biochemistry, 271, 4241-4258.

96. Leemhuis, H., Packman, L.C., Nightingale, K.P., Hollfelder, F. (2008) The human histone acetyltransferase P/CAF is a promiscuous histone propionyltransferase. Chembiochem, 9 (4), 499-503.

97. Hunt, H.V., Denyer, K., Packman, L.C., Jones, M.K., and Howe, C.J. (2010) Molecular Basis of the Waxy Endosperm Starch Phenotype in Broomcorn Millet (Panicum miliaceum L.). Mol. Biol. Evol. 27(7):1478–1494 http://mbe.oxfordjournals.org/cgi/content/full/27/7/1478

98. Christie, G., Üstok, F.I., Lu, Q., Packman, L.C. and Lowe, C.R. (2010) Mutational Analysis of Bacillus megaterium QM B1551 Cortex-Lytic Enzymes. J. Bacteriol. 192 (20), 5378-5389 http://jb.asm.org/cgi/content/full/192/20/5378