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Simone Weyand

Membrane protein structure, function and cellular activities

 

 

 

Contact Details:

Dr. Simone Weyand                                                                                                                                                             Department of Biochemistry
University of Cambridge
80 Tennis Court Road
Cambridge CB2 1GA
UK


Tel: +44 (0)1223 333676
Email: sw644@cam.ac.uk


 

                   The Weyand lab welcomes applications for postdoc positions!

I joined the Department in October 2013 and research in my group will be focusing on obtaining insights into the molecular mechanisms and cell biology of human neurotransmitter transporters and G-protein coupled receptors (GPCRs). We will be using complementary molecular, cell biological and biophysical techniques. Structural studies will be performed using crystallising thermostabilised mutants and we will determine high-resolution structures using X-ray crystallography. A combined approach of functional studies using mutational analysis and cell biological assays will complement the structural work. The combination of the structural studies with functional assays will reveal the underlying molecular mechanisms and show the nature of transport/signalling of the respective ligands. Further studies will show medically and biologically important protein substrate complexes as well as interactions with potential inhibitors. Answering the most fundamental biological questions of protein-substrate interactions in humans will enable an understanding at the atomic level. My work will allow insights towards a molecular understanding of a) neurotransmitter transporter cellular activities and b) GPCRs associated with specific types of cancer. The combination of these approaches will afford a holistic understanding of the noradrenaline transporter (NET) and dopamine transporter (DAT) in health and disease.

 

Key publications

1 Weyand, S.*, Shimamura, T.*, Yajima, S.*, Suzuki, S.*, Mirza, O.*, Krusong, K., Carpenter, E. P., Rutherford, N. G., Hadden, J. M., O’Reilly, J., Ma, P., Saidijam, M., Patching, S. G., Hope, R. J., Norbertczak, H. T., Roach, P. C. J., Iwata, S., Henderson, P. J. F. and Cameron, A. D. (2008) Structure and molecular mechanism of a nucleobase-cation-symport-1 family transporter. Science. 322(5902):709-13.


2 Shimamura,*, T., Weyand, S.*, Beckstein, O.*, Rutherford, N.G., Hadden, J.M., Sharples, D., Sansom, M.S.P, Iwata, S., Henderson, P.J.F. and Cameron, A.D. (2010): Molecular Basis of Alternating Access Membrane Transport by the Sodium-Hydantoin Transporter Mhp1, Science, 328(5977):470. (Faculty of 1000).

3 Weyand, S. and Iwata, S. (2010): Old Gate Gets a New Look, Science, 329(5988):151 – 152. (Invited Article)


4 Shimamura, T., Shiroishi, M., Weyand, S., Tsujimoto, H., Winter, G., Katritch, V., Abagyan, R., Cherezov, V., Liu, W., Han, G.W., Kobayashi, T., Stevens, R.C. and Iwata, S. (2011): Structure of the human histamine H1 receptor complex with doxepin. Nature. 475(7354):65-70.

5 Hino, T., Arakawa, T., Iwanari, H., Yurugi-Kobayashi, T., Ikeda-Suno, C., Nakada-Nakura, Y., Kusano-Arai, O., Weyand, S., Shimamura, T., Nomura, N., Cameron, A.D., Kobayashi, T., Hamakubo, T., Iwata, S. and Murata, T. (2012): Molecular basis of G-protein coupled receptor inactivation by an allosteric inverse-agonist antibody. Nature. 482(7384)237-40.