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New insight into Golgi apparatus organization

last modified Aug 18, 2014 02:09 PM

Kathryn Lilley, Paul Dupree and their colleagues have published a paper in Plant Physiology entitled "Label free protein quantification for plant Golgi protein localisation and abundance". Although the proteomic composition of the Arabidopsis Golgi apparatus was already quite well documented, little was known about the relative abundances of different proteins within this compartment. Accurate quantification of Golgi resident proteins is important as it aids understanding of the biochemical processes within this organelle, especially those of different polysaccharide synthesis pathways. Golgi proteins are hard to quantify as there are are not many of these organelles within the cell. In this study an organelle fractionation approach, targeting the Golgi apparatus, was combined with a label-free quantitative mass spectrometry (MS), data-independent acquisition (DIA) method employing ion mobility separation known as LC-IMS-MSE (or HDMSE), to simultaneously localize proteins to the Golgi apparatus and assess their relative quantity. In total 102 Golgi localised proteins were quantified. These data provide new insight into Golgi apparatus organization and demonstrate that organelle fractionation in conjunction with label free quantitative MS is a powerful and relatively simple tool to access protein organelle localisation and their relative abundances. The findings presented open a unique view on the organization of the plant Golgi apparatus, leading towards novel hypotheses centered on the biochemical processes of this organelle.