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Publications

1. Luhavaya H, Dias MV, Williams SR, Hong H, de Oliveira LG, Leadlay P.F. Enzymology of pyran ring A formation in salinomycin biosynthesis. Angew Chem Int Ed Engl 2015, Sep 17.

2. Zhou Y, Prediger P, Dias LC, Murphy AC, Leadlay P.F. Macrodiolide formation by the thioesterase of a modular polyketide synthase. Angew Chem Int Ed Engl 2015, Apr 20;54(17):5232-5.

3. Zhou Y, Prediger P, Dias LC, Murphy AC, Leadlay P.F. Macrodiolide formation by the thioesterase of a modular polyketide synthase. Angew Chem Weinheim Bergstr Ger 2015, Apr 20;127(17):5321-4.

4. Huang C, Huang F, Moison E, Guo J, Jian X, Duan X, et al. Delineating the biosynthesis of gentamicin x2, the common precursor of the gentamicin C antibiotic complex. Chem Biol 2015, Feb 19;22(2):251-61.

5. Zhou Y, Murphy AC, Samborskyy M, Prediger P, Dias LC, Leadlay P.F. Iterative mechanism of macrodiolide formation in the anticancer compound conglobatin. Chem Biol 2015, Jun 18;22(6):745-54.

6. Annaval T, Paris C, Leadlay P.F., Jacob C, Weissman KJ. Evaluating ketoreductase exchanges as a means of rationally altering polyketide stereochemistry. Chembiochem 2015, Jun 15;16(9):1357-64.

7. Riva E, Wilkening I, Gazzola S, Li WM, Smith L, Leadlay P.F., Tosin M. Chemical probes for the functionalization of polyketide intermediates. Angew Chem Int Ed Engl 2014, Oct 27;53(44):11944-9.

8. Hüttel W, Spencer JB, Leadlay P.F. Intermediates in monensin biosynthesis: A late step in biosynthesis of the polyether ionophore monensin is crucial for the integrity of cation binding. Beilstein J Org Chem 2014;10:361-8.

9. Hahn F, Kandziora N, Friedrich S, Leadlay P.F. Synthesis of complex intermediates for the study of a dehydratase from borrelidin biosynthesis. Beilstein J Org Chem 2014;10:634-40.

10. Huttel W, Spencer J, Leadlay P. Intermediates in monensin biosynthesis: A late step in biosynthesis of the polyether ionophore monensin is crucial for the integrity of cation binding. Beilstein J Org Chem 2014, Feb;10:361-8.

11. Guo J, Huang F, Huang C, Duan X, Jian X, Leeper F, et al. Specificity and promiscuity at the branch point in gentamicin biosynthesis. Chem Biol 2014, May 22;21(5):608-18.

12. Luhavaya H, Williams SR, Hong H, Gonzaga de Oliveira L, Leadlay P.F. Site-specific modification of the anticancer and antituberculosis polyether salinomycin by biosynthetic engineering. Chembiochem 2014, Sep 22;15(14):2081-5.

13. Vieweg L, Reichau S, Schobert R, Leadlay P.F., Süssmuth RD. Recent advances in the field of bioactive tetronates. Nat Prod Rep 2014, Nov;31(11):1554-84.

14. Leadlay P.F. Structural biology: Enzyme assembly line pictured. Nature 2014, Jun 26;510(7506):482-3.

15. Kanchanabanca C, Tao W, Hong H, Liu Y, Hahn F, Samborskyy M, et al. Unusual acetylation-elimination in the formation of tetronate antibiotics. Angew Chem Int Ed Engl 2013, May 27;52(22):5785-8.

16. Hong H, Fill T, Leadlay P.F. A common origin for guanidinobutanoate starter units in antifungal natural products. Angew Chem Int Ed Engl 2013, Dec 2;52(49):13096-9.

17. Guenin-Macé L, Veyron-Churlet R, Thoulouze MI, Romet-Lemonne G, Hong H, Leadlay P.F., et al. Mycolactone activation of wiskott-aldrich syndrome proteins underpins buruli ulcer formation. J Clin Invest 2013, Apr;123(4):1501-12.

18. Porter JL, Tobias NJ, Pidot SJ, Falgner S, Tuck KL, Vettiger A, et al. The cell wall-associated mycolactone polyketide synthases are necessary but not sufficient for mycolactone biosynthesis. PLoS One 2013;8(7):e70520.

19. Herrmann S, Siegl T, Luzhetska M, Petzke L, Jilg C, Welle E, et al. Site-specific recombination strategies for engineering actinomycete genomes. Appl Environ Microbiol 2012, Mar;78(6):1804-12.

20. Yurkovich ME, Tyrakis PA, Hong H, Sun Y, Samborskyy M, Kamiya K, Leadlay P.F. A late-stage intermediate in salinomycin biosynthesis is revealed by specific mutation in the biosynthetic gene cluster. Chembiochem 2012, Jan 2;13(1):66-71.

21. Moncrieffe MC, Fernandez MJ, Spiteller D, Matsumura H, Gay NJ, Luisi BF, Leadlay P.F. Structure of the glycosyltransferase eryciii in complex with its activating P450 homologue erycii. J Mol Biol 2012, Jan 6;415(1):92-101.

22. Tosin M, Smith L, Leadlay P.F. Insights into lasalocid A ring formation by chemical chain termination in vivo. Angew Chem Int Ed Engl 2011, Dec 9;50(50):11930-3.

23. Tosin M, Demydchuk Y, Parascandolo JS, Per CB, Leeper FJ, Leadlay P.F. In vivo trapping of polyketide intermediates from an assembly line synthase using malonyl carba(dethia)-n-acetyl cysteamines. Chem Commun (Camb) 2011, Mar 28;47(12):3460-2.

24. Woolard J, Vousden W, Moss SJ, Krishnakumar A, Gammons MV, Nowak DG, et al. Borrelidin modulates the alternative splicing of VEGF in favour of anti-angiogenic isoforms. Chem Sci 2011, Feb 1;2011(2):273-8.

25. Vergnolle O, Hahn F, Baerga-Ortiz A, Leadlay P.F., Andexer JN. Stereoselectivity of isolated dehydratase domains of the borrelidin polyketide synthase: Implications for cis double bond formation. Chembiochem 2011, May 2;12(7):1011-4.

26. Dickschat JS, Vergnolle O, Hong H, Garner S, Bidgood SR, Dooley HC, et al. An additional dehydratase-like activity is required for lankacidin antibiotic biosynthesis. Chembiochem 2011, Nov 4;12(16):2408-12.

27. Kwan DH, Tosin M, Schläger N, Schulz F, Leadlay P.F. Insights into the stereospecificity of ketoreduction in a modular polyketide synthase. Org Biomol Chem 2011, Apr 7;9(7):2053-6.

28. Andexer JN, Kendrew SG, Nur-e-Alam M, Lazos O, Foster TA, Zimmermann AS, et al. Biosynthesis of the immunosuppressants FK506, FK520, and rapamycin involves a previously undescribed family of enzymes acting on chorismate. Proc Natl Acad Sci U S A 2011, Mar 22;108(12):4776-81.

29. Kwan DH, Leadlay P.F. Mutagenesis of a modular polyketide synthase enoylreductase domain reveals insights into catalysis and stereospecificity. ACS Chem Biol 2010, Sep 17;5(9):829-38.

30. Lazos O, Tosin M, Slusarczyk AL, Boakes S, Cortés J, Sidebottom PJ, Leadlay P.F. Biosynthesis of the putative siderophore erythrochelin requires unprecedented crosstalk between separate nonribosomal peptide gene clusters. Chem Biol 2010, Feb 26;17(2):160-73.

31. Tosin M, Betancor L, Stephens E, Li WM, Spencer JB, Leadlay P.F. Synthetic chain terminators off-load intermediates from a type I polyketide synthase. Chembiochem 2010, Mar 1;11(4):539-46.

32. Dias MV, Huang F, Chirgadze DY, Tosin M, Spiteller D, Dry EF, et al. Structural basis for the activity and substrate specificity of fluoroacetyl-coa thioesterase flk. J Biol Chem 2010, Jul 16;285(29):22495-504.

33. Magdevska V, Gaber R, Goranovič D, Kuščer E, Boakes S, Durán Alonso MB, et al. Robust reporter system based on chalcone synthase rppa gene from saccharopolyspora erythraea. J Microbiol Methods 2010, Nov;83(2):111-9.

34. Sun Y, Hahn F, Demydchuk Y, Chettle J, Tosin M, Osada H, Leadlay P.F. In vitro reconstruction of tetronate RK-682 biosynthesis. Nat Chem Biol 2010, Feb;6(2):99-101.

35. Hong H, Leadlay P.F., Staunton J. The changing patterns of covalent active site occupancy during catalysis on a modular polyketide synthase multienzyme revealed by ion-trap mass spectrometry. FEBS J 2009, Dec;276(23):7057-69.

36. Pidot SJ, Hong H, Seemann T, Porter JL, Yip MJ, Men A, et al. Deciphering the genetic basis for polyketide variation among mycobacteria producing mycolactones. BMC Genomics 2008;9:462.

37. Truman AW, Fan Q, Röttgen M, Stegmann E, Leadlay P.F., Spencer JB. The role of cep15 in the biosynthesis of chloroeremomycin: Reactivation of an ancestral catalytic function. Chem Biol 2008, May;15(5):476-84.

38. Kwan DH, Sun Y, Schulz F, Hong H, Popovic B, Sim-Stark JC, et al. Prediction and manipulation of the stereochemistry of enoylreduction in modular polyketide synthases. Chem Biol 2008, Nov 24;15(11):1231-40.

39. Sun Y, Hong H, Gillies F, Spencer JB, Leadlay P.F. Glyceryl-S-acyl carrier protein as an intermediate in the biosynthesis of tetronate antibiotics. Chembiochem 2008, Jan 4;9(1):150-6.

40. Tran L, Tosin M, Spencer JB, Leadlay P.F., Weissman KJ. Covalent linkage mediates communication between ACP and TE domains in modular polyketide synthases. Chembiochem 2008, Apr 14;9(6):905-15.

41. Demydchuk Y, Sun Y, Hong H, Staunton J, Spencer JB, Leadlay P.F. Analysis of the tetronomycin gene cluster: Insights into the biosynthesis of a polyether tetronate antibiotic. Chembiochem 2008, May 5;9(7):1136-45.

42. Kellenberger L, Galloway IS, Sauter G, Böhm G, Hanefeld U, Cortés J, et al. A polylinker approach to reductive loop swaps in modular polyketide synthases. Chembiochem 2008, Nov 3;9(16):2740-9.

43. Betancor L, Fernández MJ, Weissman KJ, Leadlay P.F. Improved catalytic activity of a purified multienzyme from a modular polyketide synthase after coexpression with streptomyces chaperonins in escherichia coli. Chembiochem 2008, Dec 15;9(18):2962-6.

44. Smith L, Hong H, Spencer JB, Leadlay P.F. Analysis of specific mutants in the lasalocid gene cluster: Evidence for enzymatic catalysis of a disfavoured polyether ring closure. Chembiochem 2008, Dec 15;9(18):2967-75.

45. Hong H, Demangel C, Pidot SJ, Leadlay P.F., Stinear T. Mycolactones: Immunosuppressive and cytotoxic polyketides produced by aquatic mycobacteria. Nat Prod Rep 2008, Jun;25(3):447-54.

46. Petković H, Sandmann A, Challis IR, Hecht HJ, Silakowski B, Low L, et al. Substrate specificity of the acyl transferase domains of epoc from the epothilone polyketide synthase. Org Biomol Chem 2008, Feb 7;6(3):500-6.

47. Fan Q, Huang F, Leadlay P.F., Spencer JB. The neomycin biosynthetic gene cluster of streptomyces fradiae NCIMB 8233: Genetic and biochemical evidence for the roles of two glycosyltransferases and a deacetylase. Org Biomol Chem 2008, Sep 21;6(18):3306-14.

48. Schell U, Haydock SF, Kaja AL, Carletti I, Lill RE, Read E, et al. Engineered biosynthesis of hybrid macrolide polyketides containing d-angolosamine and d-mycaminose moieties. Org Biomol Chem 2008, Sep 21;6(18):3315-27.

49. te Poele EM, Samborskyy M, Oliynyk M, Leadlay P.F., Bolhuis H, Dijkhuizen L. Actinomycete integrative and conjugative pmea-like elements of amycolatopsis and saccharopolyspora decoded. Plasmid 2008, May;59(3):202-16.

50. Hong H, Coutanceau E, Leclerc M, Caleechurn L, Leadlay P.F., Demangel C. Mycolactone diffuses from mycobacterium ulcerans-infected tissues and targets mononuclear cells in peripheral blood and lymphoid organs. PLoS Negl Trop Dis 2008;2(10):e325.

51. Harvey BM, Mironenko T, Sun Y, Hong H, Deng Z, Leadlay P.F., et al. Insights into polyether biosynthesis from analysis of the nigericin biosynthetic gene cluster in streptomyces sp. DSM4137. Chem Biol 2007, Jun;14(6):703-14.

52. Hong H, Stinear T, Porter J, Demangel C, Leadlay P.F. A novel mycolactone toxin obtained by biosynthetic engineering. Chembiochem 2007, Nov 23;8(17):2043-7.

53. Harvey B, Mironenko T, Sun Y, Hong H, Deng Z, Leadlay P, et al. Insights into polyether biosynthesis from analysis of the nigericin biosynthetic gene cluster in streptomyces sp. DSM4137. Chem Biol 2007, Jun;14(6):703-14.

54. Oliynyk M, Samborskyy M, Lester JB, Mironenko T, Scott N, Dickens S, et al. Complete genome sequence of the erythromycin-producing bacterium saccharopolyspora erythraea NRRL23338. Nat Biotechnol 2007, Apr;25(4):447-53.

55. Wilkinson B, Gregory MA, Moss SJ, Carletti I, Sheridan RM, Kaja A, et al. Separation of anti-angiogenic and cytotoxic activities of borrelidin by modification at the C17 side chain. Bioorg Med Chem Lett 2006, Nov 15;16(22):5814-7.

56. Baerga-Ortiz A, Popovic B, Siskos AP, O'Hare HM, Spiteller D, Williams MG, et al. Directed mutagenesis alters the stereochemistry of catalysis by isolated ketoreductase domains from the erythromycin polyketide synthase. Chem Biol 2006, Mar;13(3):277-85.

57. O'Hare HM, Baerga-Ortiz A, Popovic B, Spencer JB, Leadlay P.F. High-throughput mutagenesis to evaluate models of stereochemical control in ketoreductase domains from the erythromycin polyketide synthase. Chem Biol 2006, Mar;13(3):287-96.

58. Gallimore AR, Stark CB, Bhatt A, Harvey BM, Demydchuk Y, Bolanos-Garcia V, et al. Evidence for the role of the monb genes in polyether ring formation during monensin biosynthesis. Chem Biol 2006, Apr;13(4):453-60.

59. Huang F, Haydock SF, Spiteller D, Mironenko T, Li TL, O'Hagan D, et al. The gene cluster for fluorometabolite biosynthesis in streptomyces cattleya: A thioesterase confers resistance to fluoroacetyl-coenzyme A. Chem Biol 2006, May;13(5):475-84.

60. Moss SJ, Carletti I, Olano C, Sheridan RM, Ward M, Math V, et al. Biosynthesis of the angiogenesis inhibitor borrelidin: Directed biosynthesis of novel analogues. Chem Commun (Camb) 2006, Jun 14(22):2341-3.

61. Harvey BM, Hong H, Jones MA, Hughes-Thomas ZA, Goss RM, Heathcote ML, et al. Evidence that a novel thioesterase is responsible for polyketide chain release during biosynthesis of the polyether ionophore monensin. Chembiochem 2006, Sep;7(9):1435-42.

62. Sheehan LS, Lill RE, Wilkinson B, Sheridan RM, Vousden WA, Kaja AL, et al. Engineering of the spinosyn PKS: Directing starter unit incorporation. J Nat Prod 2006, Dec;69(12):1702-10.

63. Sheehan L, Lill R, Wilkinson B, Sheridan R, Vousden W, Kaja A, et al. Engineering of the spinosyn PKS: Directing starter unit incorporation. J Nat Prod 2006, Dec;69(12):1702-10.

64. Sun Y, Hong H, Samborskyy M, Mironenko T, Leadlay P, Haydock S. Organization of the biosynthetic gene cluster in streptomyces sp. DSM 4137 for the novel neuroprotectant polyketide meridamycin. Microbiology 2006, Dec;152(12):3507-15.

65. Sun Y, Hong H, Samborskyy M, Samboskyy M, Mironenko T, Leadlay P.F., Haydock SF. Organization of the biosynthetic gene cluster in streptomyces sp. DSM 4137 for the novel neuroprotectant polyketide meridamycin. Microbiology 2006, Dec;152(Pt 12):3507-15.

66. Gregory MA, Hong H, Lill RE, Gaisser S, Petkovic H, Low L, et al. Rapamycin biosynthesis: Elucidation of gene product function. Org Biomol Chem 2006, Oct 7;4(19):3565-8.

67. Gregory MA, Petkovic H, Lill RE, Moss SJ, Wilkinson B, Gaisser S, et al. Mutasynthesis of rapamycin analogues through the manipulation of a gene governing starter unit biosynthesis. Angew Chem Int Ed Engl 2005, Jul 25;44(30):4757-60.

68. Bhatt A, Stark CB, Harvey BM, Gallimore AR, Demydchuk YA, Spencer JB, et al. Accumulation of an E,E,e-triene by the monensin-producing polyketide synthase when oxidative cyclization is blocked. Angew Chem Int Ed Engl 2005, Nov 4;44(43):7075-8.

69. Siskos AP, Baerga-Ortiz A, Bali S, Stein V, Mamdani H, Spiteller D, et al. Molecular basis of celmer's rules: Stereochemistry of catalysis by isolated ketoreductase domains from modular polyketide synthases. Chem Biol 2005, Oct;12(10):1145-53.

70. Hong H, Stinear T, Skelton P, Spencer JB, Leadlay P.F. Structure elucidation of a novel family of mycolactone toxins from the frog pathogen mycobacterium sp. MU128FXT by mass spectrometry. Chem Commun (Camb) 2005, Sep 14(34):4306-8.

71. Hong H, Spencer JB, Porter JL, Leadlay P.F., Stinear T. A novel mycolactone from a clinical isolate of mycobacterium ulcerans provides evidence for additional toxin heterogeneity as a result of specific changes in the modular polyketide synthase. Chembiochem 2005, Apr;6(4):643-8.

72. Hong H, Appleyard AN, Siskos AP, Garcia-Bernardo J, Staunton J, Leadlay P.F. Chain initiation on type I modular polyketide synthases revealed by limited proteolysis and ion-trap mass spectrometry. FEBS J 2005, May;272(10):2373-87.

73. Stinear TP, Hong H, Frigui W, Pryor MJ, Brosch R, Garnier T, et al. Common evolutionary origin for the unstable virulence plasmid pmum found in geographically diverse strains of mycobacterium ulcerans. J Bacteriol 2005, Mar;187(5):1668-76.

74. Haydock SF, Appleyard AN, Mironenko T, Lester J, Scott N, Leadlay P.F. Organization of the biosynthetic gene cluster for the macrolide concanamycin A in streptomyces neyagawaensis ATCC 27449. Microbiology 2005, Oct;151(Pt 10):3161-9.

75. Weissman KJ, Leadlay P.F. Combinatorial biosynthesis of reduced polyketides. Nat Rev Microbiol 2005, Dec;3(12):925-36.

76. Weissman K, Leadlay P. Combinatorial biosynthesis of reduced polyketides. Nat Rev Microbiol 2005, Dec;3(12):925-36.

77. Gregory MA, Gaisser S, Lill RE, Hong H, Sheridan RM, Wilkinson B, et al. Isolation and characterization of pre-rapamycin, the first macrocyclic intermediate in the biosynthesis of the immunosuppressant rapamycin by S. Hygroscopicus. Angew Chem Int Ed Engl 2004, May 3;43(19):2551-3.

78. Olano C, Wilkinson B, Sánchez C, Moss SJ, Sheridan R, Math V, et al. Biosynthesis of the angiogenesis inhibitor borrelidin by streptomyces parvulus tü4055: Cluster analysis and assignment of functions. Chem Biol 2004, Jan;11(1):87-97.

79. Li TL, Huang F, Haydock SF, Mironenko T, Leadlay P.F., Spencer JB. Biosynthetic gene cluster of the glycopeptide antibiotic teicoplanin: Characterization of two glycosyltransferases and the key acyltransferase. Chem Biol 2004, Jan;11(1):107-19.

80. Weissman KJ, Hong H, Oliynyk M, Siskos AP, Leadlay P.F. Identification of a phosphopantetheinyl transferase for erythromycin biosynthesis in saccharopolyspora erythraea. Chembiochem 2004, Jan 3;5(1):116-25.

81. Lowden PA, Böhm GA, Metcalfe S, Staunton J, Leadlay P.F. New rapamycin derivatives by precursor-directed biosynthesis. Chembiochem 2004, Apr 2;5(4):535-8.

82. Garcia-Bernardo J, Xiang L, Hong H, Moore BS, Leadlay P.F. Engineered biosynthesis of phenyl-substituted polyketides. Chembiochem 2004, Aug 6;5(8):1129-31.

83. Haydock SF, Mironenko T, Ghoorahoo HI, Leadlay P.F. The putative elaiophylin biosynthetic gene cluster in streptomyces sp. DSM4137 is adjacent to genes encoding adenosylcobalamin-dependent methylmalonyl coa mutase and to genes for synthesis of cobalamin. J Biotechnol 2004, Sep 30;113(1-3):55-68.

84. Boakes S, Oliynyk M, Cortés J, Böhm I, Rudd BA, Revill WP, et al. A new modular polyketide synthase in the erythromycin producer saccharopolyspora erythraea. J Mol Microbiol Biotechnol 2004;8(2):73-80.

85. Olano C, Moss SJ, Braña AF, Sheridan RM, Math V, Weston AJ, et al. Biosynthesis of the angiogenesis inhibitor borrelidin by streptomyces parvulus tü4055: Insights into nitrile formation. Mol Microbiol 2004, Jun;52(6):1745-56.

86. Stinear TP, Mve-Obiang A, Small PL, Frigui W, Pryor MJ, Brosch R, et al. Giant plasmid-encoded polyketide synthases produce the macrolide toxin of mycobacterium ulcerans. Proc Natl Acad Sci U S A 2004, Feb 3;101(5):1345-9.

87. Hughes-Thomas ZA, Stark CB, Böhm IU, Staunton J, Leadlay P.F. Intermediates released from a polyether-producing polyketide synthase provide insight into the mechanism of oxidative cyclization. Angew Chem Int Ed Engl 2003, Sep 29;42(37):4475-8.

88. Broadhurst RW, Nietlispach D, Wheatcroft MP, Leadlay P.F., Weissman KJ. The structure of docking domains in modular polyketide synthases. Chem Biol 2003, Aug;10(8):723-31.

89. Olano C, Wilkinson B, Moss SJ, Braña AF, Méndez C, Leadlay P.F., Salas JA. Evidence from engineered gene fusions for the repeated use of a module in a modular polyketide synthase. Chem Commun (Camb) 2003, Nov 21(22):2780-2.

90. Hong H, Gates PJ, Staunton J, Stinear T, Cole ST, Leadlay P.F., Spencer JB. Identification using lc-msn of co-metabolites in the biosynthesis of the polyketide toxin mycolactone by a clinical isolate of mycobacterium ulcerans. Chem Commun (Camb) 2003, Nov 21(22):2822-3.

91. Squire CM, Goss RJ, Hong H, Leadlay P.F., Staunton J. Catalytically active tetramodular 6-deoxyerythonolide B synthase fusion proteins. Chembiochem 2003, Nov 7;4(11):1225-8.

92. Petkovic H, Lill RE, Sheridan RM, Wilkinson B, McCormick EL, McArthur HA, et al. A novel erythromycin, 6-desmethyl erythromycin D, made by substituting an acyltransferase domain of the erythromycin polyketide synthase. J Antibiot (Tokyo) 2003, Jun;56(6):543-51.

93. Del Vecchio F, Petkovic H, Kendrew SG, Low L, Wilkinson B, Lill R, et al. Active-site residue, domain and module swaps in modular polyketide synthases. J Ind Microbiol Biotechnol 2003, Aug;30(8):489-94.

94. Oliynyk M, Stark CB, Bhatt A, Jones MA, Hughes-Thomas ZA, Wilkinson C, et al. Analysis of the biosynthetic gene cluster for the polyether antibiotic monensin in streptomyces cinnamonensis and evidence for the role of monb and monc genes in oxidative cyclization. Mol Microbiol 2003, Sep;49(5):1179-90.

95. Gaisser S, Kellenberger L, Kaja AL, Weston AJ, Lill RE, Wirtz G, et al. Direct production of ivermectin-like drugs after domain exchange in the avermectin polyketide synthase of streptomyces avermitilis ATCC31272. Org Biomol Chem 2003, Aug 21;1(16):2840-7.

96. Martin CJ, Timoney MC, Sheridan RM, Kendrew SG, Wilkinson B, Staunton JC, Leadlay P.F. Heterologous expression in saccharopolyspora erythraea of a pentaketide synthase derived from the spinosyn polyketide synthase. Org Biomol Chem 2003, Dec 7;1(23):4144-7.

97. Østergaard LH, Kellenberger L, Cortés J, Roddis MP, Deacon M, Staunton J, Leadlay P.F. Stereochemistry of catalysis by the ketoreductase activity in the first extension module of the erythromycin polyketide synthase. Biochemistry 2002, Feb 26;41(8):2719-26.

98. Thomas I, Martin CJ, Wilkinson CJ, Staunton J, Leadlay P.F. Skipping in a hybrid polyketide synthase. Evidence for acp-to-acp chain transfer. Chem Biol 2002, Jul;9(7):781-7.

99. Gaisser S, Martin CJ, Wilkinson B, Sheridan RM, Lill RE, Weston AJ, et al. Engineered biosynthesis of novel spinosyns bearing altered deoxyhexose substituents. Chem Commun (Camb) 2002, Mar 21(6):618-9.

100. Wilkinson CJ, Hughes-Thomas ZA, Martin CJ, Böhm I, Mironenko T, Deacon M, et al. Increasing the efficiency of heterologous promoters in actinomycetes. J Mol Microbiol Biotechnol 2002, Jul;4(4):417-26.

101. Weitnauer G, Gaisser S, Kellenberger L, Leadlay P.F., Bechthold A. Analysis of a c-methyltransferase gene (avig1) involved in avilamycin biosynthesis in streptomyces viridochromogenes tü57 and complementation of a saccharopolyspora erythraea erybiii mutant by avig1. Microbiology 2002, Feb;148(Pt 2):373-9.

102. Long PF, Wilkinson CJ, Bisang CP, Cortés J, Dunster N, Oliynyk M, et al. Engineering specificity of starter unit selection by the erythromycin-producing polyketide synthase. Mol Microbiol 2002, Mar;43(5):1215-25.

103. Gaisser S, Lill R, Staunton J, Méndez C, Salas J, Leadlay P.F. Parallel pathways for oxidation of 14-membered polyketide macrolactones in saccharopolyspora erythraea. Mol Microbiol 2002, May;44(3):771-81.

104. Lowden PA, Wilkinson B, Böhm GA, Handa S, Floss HG, Leadlay P.F., Staunton J. Origin and true nature of the starter unit for the rapamycin polyketide synthase we thank dr. Bradley S. Moore for help with the deuterium NMR experiments. This work was supported by grants from the wellcome trust (to J.S. And P.F.L.) And from the NIH (AI20264 to H.G.F.). Angew Chem Int Ed Engl 2001, Feb 16;40(4):777-9.

105. Heathcote ML, Staunton J, Leadlay P.F. Role of type II thioesterases: Evidence for removal of short acyl chains produced by aberrant decarboxylation of chain extender units. Chem Biol 2001, Feb;8(2):207-20.

106. Holzbaur IE, Ranganathan A, Thomas IP, Kearney DJ, Reather JA, Rudd BA, et al. Molecular basis of celmer's rules: Role of the ketosynthase domain in epimerisation and demonstration that ketoreductase domains can have altered product specificity with unnatural substrates. Chem Biol 2001, Apr;8(4):329-40.

107. Rowe CJ, Böhm IU, Thomas IP, Wilkinson B, Rudd BA, Foster G, et al. Engineering a polyketide with a longer chain by insertion of an extra module into the erythromycin-producing polyketide synthase. Chem Biol 2001, May;8(5):475-85.

108. Wilkinson CJ, Frost EJ, Staunton J, Leadlay P.F. Chain initiation on the soraphen-producing modular polyketide synthase from sorangium cellulosum. Chem Biol 2001, Dec;8(12):1197-208.

109. Leadlay P.F., Staunton J, Oliynyk M, Bisang C, Cortés J, Frost E, et al. Engineering of complex polyketide biosynthesis--insights from sequencing of the monensin biosynthetic gene cluster. J Ind Microbiol Biotechnol 2001, Dec;27(6):360-7.

110. Gaisser S, Lill R, Wirtz G, Grolle F, Staunton J, Leadlay P.F. New erythromycin derivatives from saccharopolyspora erythraea using sugar o-methyltransferases from the spinosyn biosynthetic gene cluster. Mol Microbiol 2001, Sep;41(5):1223-31.

111. Thomä NH, Evans PR, Leadlay P.F. Protection of radical intermediates at the active site of adenosylcobalamin-dependent methylmalonyl-coa mutase. Biochemistry 2000, Aug 8;39(31):9213-21.

112. Wilkinson B, Foster G, Rudd BA, Taylor NL, Blackaby AP, Sidebottom PJ, et al. Novel octaketide macrolides related to 6-deoxyerythronolide B provide evidence for iterative operation of the erythromycin polyketide synthase. Chem Biol 2000, Feb;7(2):111-7.

113. Bycroft M, Weissman KJ, Staunton J, Leadlay P.F. Efficient purification and kinetic characterization of a bimodular derivative of the erythromycin polyketide synthase. Eur J Biochem 2000, Jan;267(2):520-6.

114. Gaisser S, Reather J, Wirtz G, Kellenberger L, Staunton J, Leadlay P.F. A defined system for hybrid macrolide biosynthesis in saccharopolyspora erythraea. Mol Microbiol 2000, Apr;36(2):391-401.

115. Mirjalili N, Zormpaidis V, Leadlay P.F., Ison AP. The effect of rapeseed oil uptake on the production of erythromycin and triketide lactone by saccharopolyspora erythraea. Biotechnol Prog 1999, Oct 1;15(5):911-8.

116. Holzbaur IE, Harris RC, Bycroft M, Cortes J, Bisang C, Staunton J, et al. Molecular basis of celmer's rules: The role of two ketoreductase domains in the control of chirality by the erythromycin modular polyketide synthase. Chem Biol 1999, Apr;6(4):189-95.

117. Ranganathan A, Timoney M, Bycroft M, Cortés J, Thomas IP, Wilkinson B, et al. Knowledge-based design of bimodular and trimodular polyketide synthases based on domain and module swaps: A route to simple statin analogues. Chem Biol 1999, Oct;6(10):731-41.

118. Bisang C, Long PF, Cortés J, Westcott J, Crosby J, Matharu AL, et al. A chain initiation factor common to both modular and aromatic polyketide synthases. Nature 1999, Sep 30;401(6752):502-5.

119. Bisang C, Long P, Corte´s J, Westcott J, Crosby J, Matharu A, et al. A chain initiation factor common to both modular and aromatic polyketide synthases. Nature 1999, Sep;401(6752):502-5.

120. Gates PJ, Kearney GC, Jones R, Leadlay P.F., Staunton J. Structural elucidation studies of erythromycins by electrospray tandem mass spectrometry. Rapid Commun Mass Spectrom 1999;13(4):242-6.

121. Kearney GC, Gates PJ, Leadlay P.F., Staunton J, Jones R. Structural elucidation studies of erythromycins by electrospray tandem mass spectrometry II. Rapid Commun Mass Spectrom 1999;13(16):1650-6.

122. Weissman KJ, Kearney GC, Leadlay P.F., Staunton J. Structural elucidation studies of polyketide tetrasubstituted delta-lactones by gas chromatography/tandem mass spectrometry and electrospray mass spectrometry. Rapid Commun Mass Spectrom 1999;13(21):2103-8.

123. Thomä NH, Leadlay P.F. Mechanistic and structural studies on methylmalonyl-coa mutase. Biochem Soc Trans 1998, Aug;26(3):293-8.

124. Weissman KJ, Bycroft M, Staunton J, Leadlay P.F. Origin of starter units for erythromycin biosynthesis. Biochemistry 1998, Aug 4;37(31):11012-7.

125. Thomä NH, Meier TW, Evans PR, Leadlay P.F. Stabilization of radical intermediates by an active-site tyrosine residue in methylmalonyl-coa mutase. Biochemistry 1998, Oct 13;37(41):14386-93.

126. Böhm I, Holzbaur IE, Hanefeld U, Cortés J, Staunton J, Leadlay P.F. Engineering of a minimal modular polyketide synthase, and targeted alteration of the stereospecificity of polyketide chain extension. Chem Biol 1998, Aug;5(8):407-12.

127. Weissman KJ, Bycroft M, Cutter AL, Hanefeld U, Frost EJ, Timoney MC, et al. Evaluating precursor-directed biosynthesis towards novel erythromycins through in vitro studies on a bimodular polyketide synthase. Chem Biol 1998, Dec;5(12):743-54.

128. Rowe CJ, Cortés J, Gaisser S, Staunton J, Leadlay P.F. Construction of new vectors for high-level expression in actinomycetes. Gene 1998, Aug 17;216(1):215-23.

129. Pacey MS, Dirlam JP, Geldart RW, Leadlay P.F., McArthur HA, McCormick EL, et al. Novel erythromycins from a recombinant saccharopolyspora erythraea strain NRRL 2338 pig1. I. Fermentation, isolation and biological activity. J Antibiot (Tokyo) 1998, Nov;51(11):1029-34.

130. Khaw LE, Böhm GA, Metcalfe S, Staunton J, Leadlay P.F. Mutational biosynthesis of novel rapamycins by a strain of streptomyces hygroscopicus NRRL 5491 disrupted in rapl, encoding a putative lysine cyclodeaminase. J Bacteriol 1998, Feb;180(4):809-14.

131. Celis RT, Leadlay P.F., Roy I, Hansen A. Phosphorylation of the periplasmic binding protein in two transport systems for arginine incorporation in escherichia coli K-12 is unrelated to the function of the transport system. J Bacteriol 1998, Sep;180(18):4828-33.

132. Salah-Bey K, Doumith M, Michel JM, Haydock S, Cortés J, Leadlay P.F., Raynal MC. Targeted gene inactivation for the elucidation of deoxysugar biosynthesis in the erythromycin producer saccharopolyspora erythraea. Mol Gen Genet 1998, Mar;257(5):542-53.

133. Gaisser S, Böhm GA, Doumith M, Raynal MC, Dhillon N, Cortés J, Leadlay P.F. Analysis of erybi, erybiii and erybvii from the erythromycin biosynthetic gene cluster in saccharopolyspora erythraea. Mol Gen Genet 1998, Apr;258(1-2):78-88.

135. Marsden AF, Wilkinson B, Cortés J, Dunster NJ, Staunton J, Leadlay P.F. Engineering broader specificity into an antibiotic-producing polyketide synthase. Science 1998, Jan 9;279(5348):199-202.

136. Weissman KJ, Timoney M, Bycroft M, Grice P, Hanefeld U, Staunton J, Leadlay P.F. The molecular basis of celmer's rules: The stereochemistry of the condensation step in chain extension on the erythromycin polyketide synthase. Biochemistry 1997, Nov 11;36(45):13849-55.

137. Leadlay P.F. Combinatorial approaches to polyketide biosynthesis. Curr Opin Chem Biol 1997, Aug;1(2):162-8.

138. König A, Schwecke T, Molnar I, Böhm GA, Lowden PA, Staunton J, Leadlay P.F. The pipecolate-incorporating enzyme for the biosynthesis of the immunosuppressant rapamycin--nucleotide sequence analysis, disruption and heterologous expression of rapp from streptomyces hygroscopicus. Eur J Biochem 1997, Jul 15;247(2):526-34.

139. Gaisser S, Böhm GA, Cortés J, Leadlay P.F. Analysis of seven genes from the eryai-eryk region of the erythromycin biosynthetic gene cluster in saccharopolyspora erythraea. Mol Gen Genet 1997, Oct;256(3):239-51.

140. Meier TW, Thomä NH, Leadlay P.F. Tritium isotope effects in adenosylcobalamin-dependent methylmalonyl-coa mutase. Biochemistry 1996, Sep 10;35(36):11791-6.

141. Oliynyk M, Brown MJ, Cortés J, Staunton J, Leadlay P.F. A hybrid modular polyketide synthase obtained by domain swapping. Chem Biol 1996, Oct;3(10):833-9.

142. Molnár I, Aparicio JF, Haydock SF, Khaw LE, Schwecke T, König A, et al. Organisation of the biosynthetic gene cluster for rapamycin in streptomyces hygroscopicus: Analysis of genes flanking the polyketide synthase. Gene 1996, Feb 22;169(1):1-7.

143. Aparicio JF, Molnár I, Schwecke T, König A, Haydock SF, Khaw LE, et al. Organization of the biosynthetic gene cluster for rapamycin in streptomyces hygroscopicus: Analysis of the enzymatic domains in the modular polyketide synthase. Gene 1996, Feb 22;169(1):9-16.

144. Staunton J, Caffrey P, Aparicio JF, Roberts GA, Bethell SS, Leadlay P.F. Evidence for a double-helical structure for modular polyketide synthases. Nat Struct Biol 1996, Feb;3(2):188-92.

145. Thomä NH, Leadlay P.F. Homology modeling of human methylmalonyl-coa mutase: A structural basis for point mutations causing methylmalonic aciduria. Protein Sci 1996, Sep;5(9):1922-7.

146. Mancia F, Keep NH, Nakagawa A, Leadlay P.F., McSweeney S, Rasmussen B, et al. How coenzyme B12 radicals are generated: The crystal structure of methylmalonyl-coenzyme A mutase at 2 A resolution. Structure 1996, Mar 15;4(3):339-50.

147. Wiesmann KE, Cortés J, Brown MJ, Cutter AL, Staunton J, Leadlay P.F. Polyketide synthesis in vitro on a modular polyketide synthase. Chem Biol 1995, Sep;2(9):583-9.

148. Haydock SF, Aparicio JF, Molnár I, Schwecke T, Khaw LE, König A, et al. Divergent sequence motifs correlated with the substrate specificity of (methyl)malonyl-coa:Acyl carrier protein transacylase domains in modular polyketide synthases. FEBS Lett 1995, Oct 30;374(2):246-8.

149. Cortes J, Wiesmann KE, Roberts GA, Brown MJ, Staunton J, Leadlay P.F. Repositioning of a domain in a modular polyketide synthase to promote specific chain cleavage. Science 1995, Jun 9;268(5216):1487-9.

150. Aparicio JF, Caffrey P, Marsden AF, Staunton J, Leadlay P.F. Limited proteolysis and active-site studies of the first multienzyme component of the erythromycin-producing polyketide synthase. J Biol Chem 1994, Mar 18;269(11):8524-8.

151. Linton KJ, Cooper HN, Hunter IS, Leadlay P.F. An abc-transporter from streptomyces longisporoflavus confers resistance to the polyether-ionophore antibiotic tetronasin. Mol Microbiol 1994, Feb;11(4):777-85.

152. Marsden AF, Caffrey P, Aparicio JF, Loughran MS, Staunton J, Leadlay P.F. Stereospecific acyl transfers on the erythromycin-producing polyketide synthase. Science 1994, Jan 21;263(5145):378-80.

153. Morris SA, Revill WP, Staunton J, Leadlay P.F. Purification and separation of holo- and apo-forms of saccharopolyspora erythraea acyl-carrier protein released from recombinant escherichia coli by freezing and thawing. Biochem J 1993, Sep 1;294 ( Pt 2):521-7.

154. Keep NH, Smith GA, Evans MC, Diakun GP, Leadlay P.F. The synthetic substrate succinyl(carbadethia)-coa generates cob(II)alamin on adenosylcobalamin-dependent methylmalonyl-coa mutase. Biochem J 1993, Oct 15;295 ( Pt 2):387-92.

155. Bevitt DJ, Staunton J, Leadlay P.F. Mutagenesis of the dehydratase active site in the erythromycin-producing polyketide synthase. Biochem Soc Trans 1993, Feb;21(1):30S.

156. Cooper HN, Cortes J, Bevitt DJ, Leadlay P.F., Staunton J. Analysis of a gene cluster from S. Longisporoflavus potentially involved in tetronasin biosynthesis. Biochem Soc Trans 1993, Feb;21(1):31S.

157. Roberts GA, Staunton J, Leadlay P.F. 6-Deoxyerythronolide B synthase 3 from saccharopolyspora erythraea: Over-expression in escherichia coli, purification and characterisation. Biochem Soc Trans 1993, Feb;21(1):32S.

158. Leadlay P.F., Staunton J, Aparicio JF, Bevitt DJ, Caffrey P, Cortes J, et al. The erythromycin-producing polyketide synthase. Biochem Soc Trans 1993, Feb;21(1):218-22.

159. Roberts GA, Staunton J, Leadlay P.F. Heterologous expression in escherichia coli of an intact multienzyme component of the erythromycin-producing polyketide synthase. Eur J Biochem 1993, May 15;214(1):305-11.

160. Bevitt DJ, Cortes J, Haydock SF, Leadlay P.F. 6-Deoxyerythronolide-B synthase 2 from saccharopolyspora erythraea. Cloning of the structural gene, sequence analysis and inferred domain structure of the multifunctional enzyme. Eur J Biochem 1992, Feb 15;204(1):39-49.

161. Bylsma N, Drakenberg T, Andersson I, Leadlay P.F., Forsén S. Prokaryotic calcium-binding protein of the calmodulin superfamily. Calcium binding to a saccharopolyspora erythraea 20 kda protein. FEBS Lett 1992, Mar 24;299(1):44-7.

162. Caffrey P, Bevitt DJ, Staunton J, Leadlay P.F. Identification of DEBS 1, DEBS 2 and DEBS 3, the multienzyme polypeptides of the erythromycin-producing polyketide synthase from saccharopolyspora erythraea. FEBS Lett 1992, Jun 15;304(2-3):225-8.

163. Roy I, Leadlay P.F. Physical map location of the new escherichia coli gene sbm. J Bacteriol 1992, Sep;174(17):5763-4.

164. Hellyer A, Leadlay P.F., Slabas AR. Induction, purification and characterisation of acyl-acp thioesterase from developing seeds of oil seed rape (brassica napus). Plant Mol Biol 1992, Dec;20(5):763-80.

165. Caffrey P, Green B, Packman LC, Rawlings BJ, Staunton J, Leadlay P.F. An acyl-carrier-protein-thioesterase domain from the 6-deoxyerythronolide B synthase of saccharopolyspora erythraea. High-level production, purification and characterisation in escherichia coli. Eur J Biochem 1991, Feb 14;195(3):823-30.

166. Revill WP, Leadlay P.F. Cloning, characterization, and high-level expression in escherichia coli of the saccharopolyspora erythraea gene encoding an acyl carrier protein potentially involved in fatty acid biosynthesis. J Bacteriol 1991, Jul;173(14):4379-85.

167. Haydock SF, Dowson JA, Dhillon N, Roberts GA, Cortes J, Leadlay P.F. Cloning and sequence analysis of genes involved in erythromycin biosynthesis in saccharopolyspora erythraea: Sequence similarities between eryg and a family of s-adenosylmethionine-dependent methyltransferases. Mol Gen Genet 1991, Nov;230(1-2):120-8.

168. Williams KP, Leadlay P.F., Lowe PN. Inhibition of pyruvate:Ferredoxin oxidoreductase from trichomonas vaginalis by pyruvate and its analogues. Comparison with the pyruvate decarboxylase component of the pyruvate dehydrogenase complex. Biochem J 1990, May 15;268(1):69-75.

169. McKie N, Keep NH, Patchett ML, Leadlay P.F. Adenosylcobalamin-dependent methylmalonyl-coa mutase from propionibacterium shermanii. Active holoenzyme produced from escherichia coli. Biochem J 1990, Jul 15;269(2):293-8.

170. Dhillon N, Leadlay P.F. A repeated decapeptide motif in the c-terminal domain of the ribosomal RNA methyltransferase from the erythromycin producer saccharopolyspora erythraea. FEBS Lett 1990, Mar 26;262(2):189-93.

171. Cortes J, Haydock SF, Roberts GA, Bevitt DJ, Leadlay P.F. An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of saccharopolyspora erythraea. Nature 1990, Nov 8;348(6297):176-8.

172. Marsh EN, Leadlay P.F. Methylmalonyl-CoA mutase from propionibacterium shermanii. Evidence for the presence of two masked cysteine residues. Biochem J 1989, Jun 1;260(2):339-43.

173. Marsh EN, McKie N, Davis NK, Leadlay P.F. Cloning and structural characterization of the genes coding for adenosylcobalamin-dependent methylmalonyl-coa mutase from propionibacterium shermanii. Biochem J 1989, Jun 1;260(2):345-52.

174. Marsh EN, Harding SE, Leadlay P.F. Subunit interactions in propionibacterium shermanii methylmalonyl-coa mutase studied by analytical ultracentrifugation. Biochem J 1989, Jun 1;260(2):353-8.

175. Swan DG, Cortes J, Hale RS, Leadlay P.F. Cloning, characterization, and heterologous expression of the saccharopolyspora erythraea (streptomyces erythraeus) gene encoding an ef-hand calcium-binding protein. J Bacteriol 1989, Oct;171(10):5614-9.

176. Dhillon N, Hale RS, Cortes J, Leadlay P.F. Molecular characterization of a gene from saccharopolyspora erythraea (streptomyces erythraeus) which is involved in erythromycin biosynthesis. Mol Microbiol 1989, Oct;3(10):1405-14.

177. Marsh N, Leadlay P.F., Evans PR. Crystallization and preliminary diffraction data for adenosylcobalamin-dependent methylmalonyl-coa mutase from propionibacterium shermanii. J Mol Biol 1988, Mar 20;200(2):421-2.

178. Williams K, Lowe PN, Leadlay P.F. Purification and characterization of pyruvate: Ferredoxin oxidoreductase from the anaerobic protozoon trichomonas vaginalis. Biochem J 1987, Sep 1;246(2):529-36.

179. Hale RS, Jordan KN, Leadlay P.F. A small, discrete acyl carrier protein is involved in de novo fatty acid biosynthesis in streptomyces erythraeus. FEBS Lett 1987, Nov 16;224(1):133-6.

180. Swan DG, Hale RS, Dhillon N, Leadlay P.F. A bacterial calcium-binding protein homologous to calmodulin. Nature 1987;329(6134):84-5.

181. Francalanci F, Davis NK, Fuller JQ, Murfitt D, Leadlay P.F. The subunit structure of methylmalonyl-coa mutase from propionibacterium shermanii. Biochem J 1986, Jun 1;236(2):489-94.

182. Hoving H, Crysell B, Leadlay P.F. Fluorine NMR studies on stereochemical aspects of reactions catalyzed by transcarboxylase, pyruvate kinase, and enzyme I. Biochemistry 1985, Oct 22;24(22):6163-9.

183. Hale RS, Leadlay P.F. Oligonucleotide probes for bacterial acylcarrier protein genes. Biochimie 1985;67(7-8):835-9.

184. Leadlay P.F., Fuller JQ. Proton transfer in methylmalonyl-coa epimerase from propionibacterium shermanii. Studies with specifically tritiated (2R)-methylmalonyl-coa as substrate. Biochem J 1983, Sep 1;213(3):635-42.

185. Fuller JQ, Leadlay P.F. Proton transfer in methylmalonyl-coa epimerase from propionibacterium shermanii. The reaction of (2R)-methylmalonyl-coa in tritiated water. Biochem J 1983, Sep 1;213(3):643-50.

186. Leadlay P.F., Greer S. Synthesis of chiral acetic acids by a simple enzymatic procedure. Anal Biochem 1982, Nov 15;127(1):89-92.

187. Leadlay P.F. Purification and characterization of methylmalonyl-coa epimerase from propionibacterium shermanii. Biochem J 1981, Aug 1;197(2):413-9.

188. Lambiris SK, Leadlay P.F. Modification of enoyl-coa hydratase using diethyl pyrocarbonate. Biochim Biophys Acta 1981, Aug 13;660(2):271-7.

189. Armstrong J, Leadlay P.F., Perham RN. Synthesis of methyl[3H]acetimidate of high specific radioactivity, a reagent for radiolabeling proteins. Anal Biochem 1980, Dec;109(2):410-3.

190. Leadlay P.F. Free (S,S)-diaminopimelate is not an obligatory intermediate in lysine biosynthesis in corynebacterium glutamicum. FEBS Lett 1979, Feb 15;98(2):399-402.

191. Leadlay P.F., Breathnach R, Gatehouse JA, Johnson PE, Knowles JR. Phosphoglycerate mutase from wheat germ: Studies with isotopically labeled 3-phospho-d-glycerates showing that the catalyzed reaction is intramolecular. Appendix: Phosphoglycerate mutase from wheat germ: Isolation, crystallization, and properties. Biochemistry 1977, Jul 12;16(14):3045-53.

192. Leadlay P.F., Albery WJ, Knowles JR. Energetics of triosephosphate isomerase: Deuterium isotope effects in the enzyme-catalyzed reaction. Biochemistry 1976, Dec 14;15(25):5617-20.

193. Knowles JR, Leadlay P.F., Maister SG. Triosephosphate isomerase: Isotope studies on the mechanistic pathway. Cold Spring Harb Symp Quant Biol 1972;36:157-64.

 

Peter Leadlay 2010

Herchel Smith Professor of Biochemistry, Department of Biochemistry, University of Cambridge, Telephone: (01223) 333656, Fax: (01223) 766002