Although fluorescence microscopy has been widely employed as an imaging tool for biological studies, super–resolution fluorescence microscopy allows the 3D mapping of fluorescent proteins in cells with an accuracy far beyond the diffraction limit of visible light. We are currently exploiting super–resolution Photo Activation Localisation Microscopy (PALM) to directly image proteins involved in chromatin assembly and disassembly in both fixed and live cells at a near molecular resolution. This involves using molecular and cell biological techniques to tag particular proteins with photo–activatable fluorophores in order to study these proteins at the single molecule level. In preliminary studies we have investigated a model epigenetic process – the timing and deposition of the centromere–specific histone H3 variant CENP–A (Cnp1 in S. Pombe) during the cell cycle.
For further details see: Lando et al., Open Biol. 2012, 2(7):120078.
Members of our group currently involved in this project are: Dr David Lando, Dr Srinjan Basu, Edward Taylor, Yang Cao