Welcome to the Laue Group Website!
We are interested in the way chromatin structure controls epigenetic inheritance and study proteins and complexes involved in chromatin assembly/disassembly.
Our principal structural tools are NMR spectroscopy and X-ray crystallography, but we also use small-angle X-ray scattering, chemical cross-linking/mass spectrometry and biophysical methods to solve structural problems. Our current research is focussed on studies of two key protein complexes involved in chromatin structure; Chromatin Assembly Factor-1 (CAF-1), which assembles histones H3/H4 into DNA in the first step of nucleosome assembly, and the Nucleosome Remodelling Deacetylase complex (NuRD).
To complement our structural studies, we are developing approaches based on super-resolution fluorescence microscopy to directly image proteins involved in chromatin assembly and disassembly during DNA replication. This involves using molecular and cell biological techniques to tag particular proteins with photo-activatable fluorophores in order to study these proteins in live cells at the single molecule level. Finally, we are also using data from cross-linking and high-throughput sequencing to determine in-vivo chromosome structure in single cells.
Our research is funded by the Wellcome Trust, the Medical Research Council and the European Union 4D Cellfate Project. In addition, we founded and continue to play a key role in the CCPN project, a collaborative computing project for the NMR community.
Local and external collaborators:
Local: Brian Hendrich (CSCR), David Klenerman (Chemistry), Ashwin Seshia (Engineering)
External: Imre Berger (Grenoble, France), Tony Carr (Sussex), Xiuxia Du (North Carolina, USA), Ulrike Endesfelder (MPI Marburg, Germany), Opher Gileadi (SGC, Oxford), Mike Heilemann (Frankfurt, Germany), Alex Leitner (Zurich, Switzerland), David Norman (Dundee), Tom Owen-Hughes (Dundee), Frank Sobott (Antwerp, Belgium), Markus Sauer (Würzburg, Germany), Anton Wutz (CSCR, Cambridge and Zurich, Switzerland)
We welcome applications from prospective PhD students and post-doctoral researchers.