Welcome to the Laue Group Website!
We are interested in the way chromatin structure controls epigenetic inheritance and study proteins and complexes involved in chromatin assembly/disassembly.
Our principal structural tools are NMR spectroscopy and X-ray crystallography, but we also use small-angle X-ray scattering, chemical cross-linking/mass spectrometry and biophysical methods to solve structural problems. Our current research is focussed on studies of two key protein complexes involved in chromatin structure; Chromatin Assembly Factor-1 (CAF-1), which assembles histones H3/H4 into DNA in the first step of nucleosome assembly, and the Nucleosome Remodelling Deacetylase complex (NuRD).
To complement our structural studies, we are developing approaches based on super-resolution fluorescence microscopy to directly image proteins involved in chromatin assembly and disassembly during DNA replication. This involves using molecular and cell biological techniques to tag particular proteins with photo-activatable fluorophores in order to study these proteins in live cells at the single molecule level. Finally, we are also using data from cross-linking and high-throughput sequencing to determine in-vivo chromosome structure in single cells.
Our research is funded by the Wellcome Trust and the European Union 4D Cellfate Project. In addition, we founded and continue to play a key role in the CCPN project, a collaborative computing project for the NMR community.
Local and external collaborators:
Local: Peter Fraser (Babraham Institute), Brian Hendrich (CSCR), David Klenerman (Chemistry), Ashwin Seshia (Engineering)
External: Imre Berger (Grenoble, France), Tony Carr (Sussex), Xiuxia Du (North Carolina, USA), Opher Gileadi (SGC, Oxford), Mike Heilemann (Frankfurt, Germany), Alex Leitner (Zurich, Switzerland), David Norman (Dundee), Tom Owen-Hughes (Dundee), Frank Sobott (Antwerp, Belgium), Markus Sauer (Würzburg, Germany), Anton Wutz (CSCR, Cambridge and Zurich, Switzerland)
We welcome applications from prospective PhD students and post-doctoral researchers.