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Hollfelder Group Publications

For the very latest publications check on PubMed through this link (opens a new window).

 

2016 / 2015 / 2014 / 2013 / 2012 / 2011 / 2010 / 2009 / 2008 / 2007 / 2006 / Before 2005

 

2016

107.

Kaltenbach, M.; Emond, S.; Hollfelder, F.; Tokuriki, N., Functional trade-offs in promiscuous enzymes cannot be explained by intrinsic mutational robustness of the native activity. PLOS Genetics 2016 (in press)

106.

Sum, T.H.; Sum, T.J.; Galloway, W.R.; Collins, S.; Twigg, D.G.; Hollfelder, F; Spring, D.R., Combinatorial Synthesis of Structurally Diverse Triazole-Bridged Flavonoid Dimers and Trimers. Molecules 2016, 21(9), 1230. Read online

105.

Campbell, E.; Kaltenbach, M.; Correy, G.J.; Carr, P.D.; Porebski, B.T.; Livingstone, E.K.; Afriat-Jurnou, L.; Buckle, A.M.; Weik, M.; Hollfelder, F.; Tokuriki, N.; Jackson, C.J., The role of protein dynamics in the evolution of new enzyme function. Nature Chemical Biology 2016 (advance online publication). Read online

2015

104.

Colin, P.Y.; Kintses, B.; Gielen, F.; Miton, C.M.; Fischer, G.; Mohamed, M.F.; Hyvönen, M.; Morgavi, D.P.; Janssen, D.B.; Hollfelder, F., Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics. Nature Communications 2015, 6:10008. Read online

103.

Urbach, C.; Gordon, N.C.; Strickland, I.; Lowne, D.; Joberty-Candotti, C.; May, R.; Herath, A.; Hijnen, D.; Thijs, J.L.; Bruijnzeel-Koomen C.A.; Minter, R.R.; Hollfelder, F.; Jermutus, L., Combinatorial Screening Identifies Novel Promiscuous Matrix Metalloproteinase Activities that Lead to Inhibition of the Therapeutic Target IL-13. Chem Biol 2015, 22(11), 1442-1452. Read online

102.

Fradet, E.; Bayer, C.D.; Hollfelder, F.; Baroud, C.N., Measuring fast and slow enzyme kinetics in stationary droplets. Anal Chem 2015, 87(23), 11915–11922. Read online

101.

Colin, P.Y.; Zinchenko, A.; Hollfelder, F., Enzyme engineering in biomimetic compartments. Curr Opin Struct Biol 2015, 33, 42-51. Read online

100.

Kaltenbach, M.; Jackson, C.J.; Campbell, E.C.; Hollfelder, F., Tokuriki, N., Reverse evolution leads to genotypic incompatibility despite functional and active-site convergence. Elife 2015, eLife06492. Read online

99.

Houlihan, G.; Gatti-Lafranconi, P.; Lowe, D.; Hollfelder, F., Directed evolution of anti-HER2 DARPins by SNAP display reveals stability/function trade-offs in the selection process. Protein Eng Des Sel 2015, 28(9), 269-279. Read online

98.

Shafee, T.; Gatti-Lafranconi, P.; Minter, R.; Hollfelder, F., Handicap-recover evolution leads to a chemically versatile, nucleophile-permissive protease. ChemBioChem 2015, 16(13), 1866–1869. Read online

97.

van Vliet, L. D.; Colin, P.Y.; Hollfelder, F., Bioinspired genotype–phenotype linkages: mimicking cellular compartmentalization for the engineering of functional proteins.  Interface Focus 2015, 5(4), 20150035. Read online

96.

Chen, W.; Avezov, E.; Schlachter, S. C.; Gielen, F.; Laine, R. F.; Harding, H. P.; Hollfelder, F.; Ron, D.; Kaminski, C. F., A method to quantify FRET stoichiometry with phasor plot analysis and acceptor lifetime in growth. Biophys J. 2015, 108(5), 999-1002. Read online

95.

Gielen, F.; Buryska, T.; Van Vliet ,L.; Butz, M.; Damborsky, J.; Prokop, Z.; Hollfelder, F., Interfacing microwells with nanoliter compartments: a sampler generating high-resolution concentration gradients for quantitative biochemical analyses in droplets. Anal Chem. 2015, 87(1), 624-32. Read online

2014

94.

Zinchenko, A.; Devenish, S. R.; Kintses, B.; Colin, P. Y.; Fischlechner, M.; Hollfelder, F., One in a Million: Flow Cytometric Sorting of Single Cell-Lysate Assays in Monodisperse Picolitre Double Emulsion Droplets for Directed Evolution. Anal Chem 2014, 86 (5), 2526-33. Read online

93.

Villiers, B.; Hollfelder, F., USER friendly DNA recombination (USERec): gene library construction requiring minimal sequence homology. Methods Mol Biol 2014, 1179, 213-24. Read online

92.

Houlihan, G.; Gatti-Lafranconi, P.; Kaltenbach, M.; Lowe, D.; Hollfelder, F., An experimental framework for improved selection of binding proteins using SNAP display. J Immunol Methods 2014,  (405), 47-56. Read online

91.

Fischlechner, M.; Schaerli, Y.; Mohamed, M. F.; Patil, S.; Abell, C.; Hollfelder, F., Evolution of enzyme catalysts caged in biomimetic gel-shell beads. Nat Chem 2014, 6 (9), 791-6. Read online

2013

90.

Yan, J.; Bauer, W. A. C.; Fischlechner, M.; Hollfelder, F.; Kaminski, C. F.; Huck, W. T. S., Monodisperse Water-in-Oil-in-Water (W/O/W) Double Emulsion Droplets as Uniform Compartments for High-Throughput Analysis via Flow Cytometry. Micromachines 2013, 4 (4), 402-413. Read online

89.

Shim, J. U.; Ranasinghe, R. T.; Smith, C. A.; Ibrahim, S. M.; Hollfelder, F.; Huck, W. T.; Klenerman, D.; Abell, C., Ultrarapid generation of femtoliter microfluidic droplets for single-molecule-counting immunoassays. ACS Nano 2013, 7 (7), 5955-64. Read online

88.

Schober, M.; Toesch, M.; Knaus, T.; Strohmeier, G. A.; van Loo, B.; Fuchs, M.; Hollfelder, F.; Macheroux, P.; Faber, K., One-pot deracemization of sec-alcohols: enantioconvergent enzymatic hydrolysis of alkyl sulfates using stereocomplementary sulfatases. Angew Chem Int Ed Engl 2013, 52 (11), 3277-9. Read online

87.

Mohamed, M. F.; Hollfelder, F., Efficient, crosswise catalytic promiscuity among enzymes that catalyze phosphoryl transfer. Biochim Biophys Acta 2013, 1834 (1), 417-24. Read online

86.

Kwok, A.; Eggimann, G. A.; Reymond, J. L.; Darbre, T.; Hollfelder, F., Peptide dendrimer/lipid hybrid systems are efficient DNA transfection reagents: structure--activity relationships highlight the role of charge distribution across dendrimer generations. ACS Nano 2013, 7 (5), 4668-82. Read online

85.

Houlihan, G.; Lowe, D.; Hollfelder, F., SNAP display - an in vitro method for the selection of protein binders. Curr Pharm Des 2013, 19 (30), 5421-8. Read online

84.

Hollfelder, F.; Lutz, S., Just (protein) engineering? Curr Opin Struct Biol 2013, 23 (4), 569-70. Read online

83.

Goldsworthy, M.; Absalom, N. L.; Schroter, D.; Matthews, H. C.; Bogani, D.; Moir, L.; Long, A.; Church, C.; Hugill, A.; Anstee, Q. M.; Goldin, R.; Thursz, M.; Hollfelder, F.; Cox, R. D., Mutations in Mll2, an H3K4 methyltransferase, result in insulin resistance and impaired glucose tolerance in mice. PLoS One 2013, 8 (6), e61870. Read online

82.

Gielen, F.; van Vliet, L.; Koprowski, B. T.; Devenish, S. R.; Fischlechner, M.; Edel, J. B.; Niu, X.; deMello, A. J.; Hollfelder, F., A fully unsupervised compartment-on-demand platform for precise nanoliter assays of time-dependent steady-state enzyme kinetics and inhibition. Anal Chem 2013, 85 (9), 4761-9. Read online

81.

Gatti-Lafranconi, P.; Hollfelder, F., Flexibility and reactivity in promiscuous enzymes. Chembiochem 2013, 14 (3), 285-92. Read online

80.

Gatti-Lafranconi, P.; Dijkman, W. P.; Devenish, S. R.; Hollfelder, F., A single mutation in the core domain of the lac repressor reduces leakiness. Microb Cell Fact 2013, 12, 67. Read online

79.

Diamante, L.; Gatti-Lafranconi, P.; Schaerli, Y.; Hollfelder, F., In vitro affinity screening of protein and peptide binders by megavalent bead surface display. Protein Eng Des Sel 2013, 26 (10), 713-24. Read online

78.

Devenish, S. R.; Kaltenbach, M.; Fischlechner, M.; Hollfelder, F., Droplets as reaction compartments for protein nanotechnology. Methods Mol Biol 2013, 996, 269-86. Read online

2012 (back to top)

77.

Stein, V.; Kaltenbach, M.; Hollfelder, F., Assembling linear DNA templates for in vitro transcription and translation. Methods Mol Biol (Functional Genomics: Methods and Protocols) 2012, 815, 67-78. Read online

76.

Schaerli, Y.; Kintses, B.; Hollfelder, F., Protein Engineering in Microdroplets. In Protein Engineering Handbook, Lutz, S.; Bornscheuer, U. T., Eds. Wiley VCH: Weinheim, 2012; Vol. 3, pp 73-85.

75.

Kintses, B.; Hein, C.; Mohamed, M. F.; Fischlechner, M.; Courtois, F.; Laine, C.; Hollfelder, F., Picoliter cell lysate assays in microfluidic droplet compartments for directed enzyme evolution. Chem Biol 2012, 19 (8), 1001-9. Read online

74.

Kaltenbach, M.; Hollfelder, F., SNAP display: in vitro protein evolution in microdroplets. Methods Mol Biol (Ribosome Display and Related Technologies: Methods and Protocols) 2012, 805, 101-11. Read online

73.

Kaltenbach, M.; Devenish, S. R.; Hollfelder, F., A simple method to evaluate the biochemical compatibility of oil/surfactant mixtures for experiments in microdroplets. Lab Chip 2012, 12 (20), 4185-92. Read online

72.

Delley, R. J.; Bandyopadhyay, S.; Fox, M. A.; Schliehe, C.; Hodgson, D. R.; Hollfelder, F.; Kirby, A. J.; O'Donoghue, A. C., peri-Dimethylamino substituent effects on proton transfer at carbon in alpha-naphthylacetate esters: a model for mandelate racemase. Org Biomol Chem 2012, 10 (3), 590-6. Read online

71.

Babtie, A. C.; Lima, M. F.; Kirby, A. J.; Hollfelder, F., Kinetic and computational evidence for an intermediate in the hydrolysis of sulfonate esters. Org Biomol Chem 2012. Read online

2011 (back to top)

70.

Villiers, B.; Hollfelder, F., Directed evolution of a gatekeeper domain in nonribosomal peptide synthesis. Chem Biol 2011, 18 (10), 1290-9. Read online

69.

Shim, J. U.; Patil, S. N.; Hodgkinson, J. T.; Bowden, S. D.; Spring, D. R.; Welch, M.; Huck, W. T.; Hollfelder, F.; Abell, C., Controlling the contents of microdroplets by exploiting the permeability of PDMS. Lab Chip 2011, 11 (6), 1132-7. Read online

68.

Kaltenbach, M.; Stein, V.; Hollfelder, F., SNAP dendrimers: multivalent protein display on dendrimer-like DNA for directed evolution. Chembiochem 2011, 12 (14), 2208-16. Read online

67.

Huebner, A. M.; Abell, C.; Huck, W. T.; Baroud, C. N.; Hollfelder, F., Monitoring a reaction at submillisecond resolution in picoliter volumes. Anal Chem 2011, 83 (4), 1462-8. Read online

66.

Hollfelder, F., From fascination to function. Curr Opin Biotechnol 2011, 22 (4), 473-4. Read online

2010 (back to top)

65.

Villiers, B. R.; Stein, V.; Hollfelder, F., USER friendly DNA recombination (USERec): a simple and flexible near homology-independent method for gene library construction. Protein Eng Des Sel 2010, 23 (1), 1-8. Read online

64.

van Loo, B.; Jonas, S.; Babtie, A. C.; Benjdia, A.; Berteau, O.; Hyvönen, M.; Hollfelder, F., An efficient, multiply promiscuous hydrolase in the alkaline phosphatase superfamily. Proc. Natl Acad. Sci USA 2010, 107 (7), 2740-5. Read online

63.

van Loo, B.; Hollfelder, F., Enzyme promiscuity and evolution of new protein functions. In Manual of Industrial Microbiology and Biotechnology, 3rd ed.; Zhao, H., Ed. American Society for Microbiology: Washington, 2010; Vol. 3rd, pp 524-538.

62.

Theberge, A. B.; Courtois, F.; Schaerli, Y.; Fischlechner, M.; Abell, C.; Hollfelder, F.; Huck, W. T., Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology. Angew Chem Int Ed Engl 2010, 49 (34), 5846-68. Read online

61.

Schaerli, Y.; Stein, V.; Spiering, M. M.; Benkovic, S. J.; Abell, C.; Hollfelder, F., Isothermal DNA amplification using the T4 replisome: circular nicking endonuclease-dependent amplification and primase-based whole-genome amplification. Nucleic Acids Res 2010, 38 (22), e201. Read online

60.

O’Brien, P. J.; Hollfelder, F., Hitting a moving target?—understanding how conformational diversity impacts enzymatic catalysis. Curr. Op. Chem. Biol. 2010, 14 (5), 634-5. Read online

59.

Kintses, B.; van Vliet, L. D.; Devenish, S. R.; Hollfelder, F., Microfluidic droplets: new integrated workflows for biological experiments. Curr Opin Chem Biol 2010, 14 (5), 548-55. Read online

58.

Bai, Y.; He, X.; Liu, D.; Patil, S. N.; Bratton, D.; Huebner, A.; Hollfelder, F.; Abell, C.; Huck, W. T. S., A double droplet trap system for studying mass transport across a droplet-droplet interface. Lab Chip 2010, 10 (10), 1281-5. Read online

57.

Babtie, A. C.; Tokuriki, N.; Hollfelder, F., What makes an enzyme promiscuous? Curr. Op. Chem. Biol 2010, 14 (2), 200-7. Read online

2009 (back to top)

56.

Villiers, B. R. M.; Hollfelder, F., Mapping the Limits of Substrate Specificity of the Adenylation Domain of TycA. Chembiochem 2009, 10 (4), 671-682. Read online

55.

Stein, V.; Hollfelder, F., An efficient method to assemble linear DNA templates for in vitro screening and selection systems. Nucleic Acids Res 2009, 37 (18), e122. Read online

54.

Shim, J. U.; Olguin, L. F.; Whyte, G.; Scott, D.; Babtie, A.; Abell, C.; Huck, W. T.; Hollfelder, F., Simultaneous determination of gene expression and enzymatic activity in individual bacterial cells in microdroplet compartments. J Am Chem Soc 2009, 131 (42), 15251-6. Read online

53.

Schaerli, Y.; Wootton, R. C.; Robinson, T.; Stein, V.; Dunsby, C.; Neil, M. A. A.; French, P. M. W.; deMello, A. J.; Abell, C.; Hollfelder, F., Continuous-Flow Polymerase Chain Reaction of Single-Copy DNA in Microfluidic Microdroplets. Analytical Chemistry 2009, 81 (1), 302-306. Read online

52.

Schaerli, Y.; Hollfelder, F., The potential of microfluidic water-in-oil droplets in experimental biology. Mol Bio Sys 2009, 5 (12), 1392-404. Read online

51.

Robinson, T.; Schaerli, Y.; Wootton, R.; Hollfelder, F.; Dunsby, C.; Baldwin, G.; Neil, M.; French, P.; Demello, A., Removal of background signals from fluorescence thermometry measurements in PDMS microchannels using fluorescence lifetime imaging. Lab Chip 2009, 9 (23), 3437-41. Read online

50.

Kirby, A. J.; Hollfelder, F., From Enzyme Models to Model Enzymes. Royal Society of Chemistry Cambridge, 2009; p 228. Read online

49.

Kaltenbach, M.; Schärli, Y.; Hollfelder, F., Microdroplets - A Tool for Protein Engineering. BioForum 2009, 13, 19-21.

48.

Jonas, S.; Hollfelder, F., Mapping catalytic promiscuity in the alkaline phosphatase superfamily. Pure and Applied Chemistry 2009, 81 (4), 731-742. Read online

47.

Hufnagel, H.; Huebner, A.; Gulch, C.; Guse, K.; Abell, C.; Hollfelder, F., An integrated cell culture lab on a chip: modular microdevices for cultivation of mammalian cells and delivery into microfluidic microdroplets. Lab on a Chip 2009, 9 (11), 1576-1582. Read online

46.

Hufnagel, H.; Hakim, P.; Lima, A.; Hollfelder, F., Fluid Phase Endocytosis Contributes to Transfection of DNA by PEI-25. Mol. Therapy 2009, 17 (8), 1411-7. Read online

45.

Huebner, A.; Bratton, D.; Whyte, G.; Yang, M.; deMello, A. J.; Abell, C.; Hollfelder, F., Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays. Lab on a Chip 2009, 9 (5), 692-698. Read online

44.

Golicnik, M.; Olguin, L. F.; Feng, G.; Baxter, N. J.; Waltho, J. P.; Williams, N. H.; Hollfelder, F., Kinetic analysis of beta-phosphoglucomutase and its inhibition by magnesium fluoride. J Am Chem Soc 2009, 131 (4), 1575-88. Read online

43.

Damean, N.; Olguin, L. F.; Hollfelder, F.; Abell, C.; Huck, W. T. S., Simultaneous measurement of reactions in microdroplets filled by concentration gradients. Lab on a Chip 2009, 9 (12), 1707-1713. Read online

42.

Courtois, F.; Olguin, L. F.; Whyte, G.; Theberge, A. B.; Huck, W. T. S.; Hollfelder, F.; Abell, C., Controlling the Retention of Small Molecules in Emulsion Microdroplets for Use in Cell-Based Assays. Analytical Chemistry 2009, 81 (8), 3008-3016. Read online

41.

Courtois, F.; Huebner, A.; Stein, V.; Olguin, L.; Hollfelder, F., Towards biological experimentation in microfluidic microdroplets. La Houille Blanche 2009, 5, 127-133. Read online

40.

Babtie, A. C.; Bandyopadhyay, S.; Olguin, L. F.; Hollfelder, F., Efficient Catalytic Promiscuity for Chemically Distinct Reactions. Angewandte Chemie-International Edition 2009, 48 (20), 3692-3694. Read online

39.

Avenier, F.; Hollfelder, F., Combining Medium Effects and Cofactor Catalysis: Metal-Coordinated Synzymes Accelerate Phosphate Transfer by 10(8). Chemistry-a European Journal 2009, 15 (45), 12371-12380. Read online

2008 (back to top)

38.

Van Vliet, L. D.; Chapman, M. R.; Avenier, F.; Kitson, C. Z.; Hollfelder, F., Relating chemical and biological diversity space: A tunable system for efficient gene transfection. Chembiochem 2008, 9 (12), 1960-1967. Read online

37.

Shim, J. U.; Olguin, L.; Hollfelder, F.; Abell, C.; Huck, W., Cell based biological assays using microfluidics. Ekc2008: Proceedings of the Eu-Korea Conference on Science and Technology 2008, 124, 499-502. Read online

36.

Olguin, L. F.; Askew, S. E.; O'Donoghue, A. C.; Hollfelder, F., Efficient Catalytic Promiscuity in an Enzyme Superfamily: An Arylsulfatase Shows a Rate Acceleration of 10(13) for Phosphate Monoester Hydrolysis. Journal of the American Chemical Society 2008, 130 (49), 16547-16555. Read online

35.

Leemhuis, H.; Packman, L. C.; Nightingale, K. P.; Hollfelder, F., The human histone acetyltransferase P/CAF is a promiscuous histone propionyltransferase. Chembiochem 2008, 9 (4), 499-503. Read online

34.

Leemhuis, H.; Nightingale, K. P.; Hollfelder, F., Directed evolution of a histone acetyltransferase - enhancing thermostability, whilst maintaining catalytic activity and substrate specificity. The FEBS Journal 2008, 275 (22), 5635-5647. Read online

33.

Kirby, A. J.; Hollfelder, F., Biochemistry: Enzymes under the nanoscope. Nature 2008, 456 (7218), 45. Read online

32.

Jonas, S.; van Loo, B.; Hyvonen, M.; Hollfelder, F., A New Member of the Alkaline Phosphatase Superfamily with a Formylglycine Nucleophile: Structural and Kinetic Characterisation of a Phosphonate Monoester Hydrolase/Phosphodiesterase from Rhizobium leguminosarum. Journal of Molecular Biology 2008, 384 (1), 120-136. Read online

31.

Jonas, S.; Hollfelder, F., Mechanism and Catalytic Promiscuity: Emerging Mechanistic Principles for Identification and Manipulation of Catalytically Promiscuous Enzymes. In The Handbook of Protein Engineering, Bornscheuer, U. T.; Lutz, S., Eds. Wiley-VCH: Chichester, 2008; Vol. 1, pp 47–72. Read online

30.

Huebner, A.; Sharma, S.; Srisa-Art, M.; Hollfelder, F.; Edel, J. B.; Demello, A. J., Microdroplets: A sea of applications? Lab on a Chip 2008, 8 (8), 1244-1254. Read online

29.

Huebner, A.; Olguin, L. F.; Bratton, D.; Whyte, G.; Huck, W. T. S.; de Mello, A. J.; Edel, J. B.; Abell, C.; Hollfelder, F., Development of quantitative cell-based enzyme assays in microdroplets. Analytical Chemistry 2008, 80 (10), 3890-3896. Read online

28.

Courtois, F.; Olguin, L. F.; Whyte, G.; Bratton, D.; Huck, W. T. S.; Abell, C.; Hollfelder, F., An integrated device for monitoring time-dependent in vitro expression from single genes in picolitre droplets. Chembiochem 2008, 9 (3), 439-446. Read online

27.

Baxter, N. J.; Blackburn, G. M.; Marston, J. P.; Hounslow, A. M.; Cliff, M. J.; Bermel, W.; Williams, N. H.; Hollfelder, F.; Wemmer, D. E.; Waltho, J. P., Anionic charge is prioritized over geometry in aluminum and magnesium fluoride transition state analogs of phosphoryl transfer enzymes. Journal of the American Chemical Society 2008, 130 (12), 3952-3958. Read online

2007 (back to top)

26.

Van Vliet, L. D.; Ellis, T.; Foley, P. J.; Liu, L. G.; Pfeffer, F. M.; Russell, R. A.; Warrener, R. N.; Hollfelder, F.; Waring, M. J., Molecular recognition of DNA by rigid [n]-polynorbornane-derived bifunctional intercalators: Synthesis and evaluation of their binding properties. Journal of Medicinal Chemistry 2007, 50 (10), 2326-2340. Read online

25.

Stein, V.; Sielaff, I.; Johnsson, K.; Hollfelder, F., A covalent chemical genotype-phenotype linkage for in vitro protein evolution. Chembiochem 2007, 8 (18), 2191-2194. Read online

24.

Otten, L. G.; Schaffer, M. L.; Villiers, B. R.; Stachelhaus, T.; Hollfelder, F., An optimized ATP/PP(i)-exchange assay in 96-well format for screening of adenylation domains for applications in combinatorial biosynthesis. Biotechnol J 2007, 2 (2), 232-40. Read online

23.

Nightingale, K. P.; Gendreizig, S.; White, D. A.; Bradbury, C.; Hollfelder, F.; Turner, B. M., Cross-talk between histone modifications in response to histone deacetylase inhibitors - MLL4 links histone H3 acetylation and histone H3K4 methylation. Journal of Biological Chemistry 2007, 282 (7), 4408-4416. Read online

22.

Huebner, A.; Srisa-Art, M.; Holt, D.; Abell, C.; Hollfelder, F.; Demello, A. J.; Edel, J. B., Quantitative detection of protein expression in single cells using droplet microfluidics. Chemical Communications 2007,  (12), 1218-1220. Read online

21.

Avenier, F.; Domingos, J. B.; Van Vliet, L. D.; Hollfelder, F., Polyethylene imine derivatives ('Synzymes') accelerate phosphate transfer in the absence of metal. Journal of the American Chemical Society 2007, 129 (24), 7611-7619. Read online

2006 (back to top)

20.

Mathonet, P.; Leemhuis, H.; Griffiths, A. D.; Hollfelder, F., Towards compartmentalised selections of protein-protein interactions using a split-protein sensor. FEBS Journal 2006, 273, 253-253.

19.

Baxter, N. J.; Olguin, L. F.; Golicnik, M.; Feng, G.; Hounslow, A. M.; Bermel, W.; Blackburn, G. M.; Hollfelder, F.; Waltho, J. P.; Williams, N. H., A Trojan horse transition state analogue generated by MgF3- formation in an enzyme active site. Proceedings of the National Academy of Sciences of the United States of America 2006, 103 (40), 14732-14737. Read online

Before 2005 (back to top)

18.

Leemhuis, H.; Stein, V.; Griffiths, A. D.; Hollfelder, F., New genotype-phenotype linkages for directed evolution of functional proteins. Current Opinion in Structural Biology 2005, 15 (4), 472-478. Read online

17.

Kirby, A. J.; Gesser, J. C.; Hollfelder, F.; Priebe, J. P.; Nome, F., Intramolecular general acid catalysis of sulfate transfer - Nucleophilic attack by oxyanions on the SO3- group. Canadian Journal of Chemistry-Revue Canadienne De Chimie 2005, 83 (9), 1629-1636. Read online

16.

Lindner, A.; Hollfelder, F., The Evolution of Catalysis. ChemBioChem 2004, 5, 241. Read online

15.

Guiry, P. J.; Hollfelder, F., Highlights from the 37th ESF/EUCHEM conference on stereochemistry, Bürgenstock, Switzerland, . Chem Commun (Camb) 2002, 21, XVIII-XIX. Read online

14.

Zamble, D. B.; Miller, D. A.; Heddle, J. G.; Maxwell, A.; Walsh, C. T.; Hollfelder, F., In vitro characterization of DNA gyrase inhibition by microcin B17 analogs with altered bisheterocyclic sites. Proceedings of the National Academy of Sciences of the United States of America 2001, 98 (14), 7712-7717. Read online

13.

Hollfelder, F.; Kirby, A. J.; Tawfik, D. S., On the magnitude and specificity of medium effects in enzyme-like catalysts for proton transfer. Journal of Organic Chemistry 2001, 66 (17), 5866-5874. Read online

12.

Heddle, J. G.; Blance, S. J.; Zamble, D. B.; Hollfelder, F.; Miller, D. A.; Wentzell, L. M.; Walsh, C. T.; Maxwell, A., The antibiotic microcin B17 is a DNA gyrase poison: Characterisation of the mode of inhibition. Journal of Molecular Biology 2001, 307 (5), 1223-1234. Read online

11.

Perez-Juste, J.; Hollfelder, F.; Kirby, A. J.; Engberts, J. B. F. N., Vesicles accelerate proton transfer from carbon up to 850-fold. Organic Letters 2000, 2 (2), 127-130. Read online

10.

Kirby, A. J.; Hollfelder, F.; Tawfik, D. S., Nonspecific catalysis by protein surfaces. Applied Biochemistry and Biotechnology 2000, 83 (1-3), 173-180. Read online

9.

Hollfelder, F.; Kirby, A. J.; Tawfik, D. S.; Kikuchi, K.; Hilvert, D., Characterization of proton-transfer catalysis by serum albumins. Journal of the American Chemical Society 2000, 122 (6), 1022-1029. Read online

8.

Hollfelder, F.; Kirby, A. J.; Tawfik, D. S., Efficient catalysis of proton transfer by synzymes. Journal of the American Chemical Society 1997, 119 (40), 9578-9579. Read online

7.

Genre-Grandpierre, A.; Tellier, C.; Loirat, M. J.; Blanchard, D.; Hodgson, D. R. W.; Hollfelder, F.; Kirby, A. J., Catalysis of the Kemp elimination by antibodies elicited against a cationic hapten. Bioorganic & Medicinal Chemistry Letters 1997, 7 (19), 2497-2502. Read online

6.

Hollfelder, F.; Kirby, A. J.; Tawfik, D. S., Off-the-shelf proteins that rival tailor-made antibodies as catalysts. Nature 1996, 383 (6595), 60-63. Read online

5.

Hollfelder, F.; Herschlag, D., The Nature of the Transition-State for Enzyme-Catalyzed Phosphoryl Transfer - Hydrolysis of O-Aryl Phosphorothioates by Alkaline-Phosphatase. Biochemistry 1995, 34 (38), 12255-12264. Read online

4.

Dalby, K. N.; Kirby, A. J.; Hollfelder, F., Simple but Efficient Models for Nuclease Catalysis. Pure and Applied Chemistry 1994, 66 (4), 687-694. Read online

3.

Chandler, A. J.; Hollfelder, F.; Kirby, A. J.; Ocarroll, F.; Stromberg, R., Models for Enzyme-Catalyzed Phosphate Transfer - Comparisons of Reactivity Towards a Neighboring Hydroxy Group for Phosphodiester Anions and Acids - General Base Catalysis of the Cyclization of a Hydroxyalkyl Phosphate Triester. Journal of the Chemical Society-Perkin Transactions 2 1994,  (2), 327-333. Read online

2.

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Picodroplet functional metagenomics

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