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Hollfelder group

Stephane Emond

Joined the lab: April 2010
Background and Current Projects: After receiving my MSc in Biochemical Engineering from INSA Toulouse (France), I did an industrial PhD (2004-2007) with a biotech company (MilleGen, Toulouse) under the academic supervision of Prof Pierre Monsan, where I worked on developing random mutagenesis approaches using error-prone DNA polymerases and engineering glycoside hydrolases (amylosucrase) using directed evolution. I then joined the group of Prof Magali Remaud-Simeon where I worked on different projects involving lipases and esterases. I joined the Hollfelder group in 2010 and my current work investigates the effects of insertions and deletions (InDels) in proteins using laboratory evolution approaches.

[11] Emond S., Socha R., Tokuriki N. (2015). Strategies to Overcome Stability Constraints in Enzyme Evolution and Facilitate Effective Enzyme Engineering, in "Industrial Biocatalysis" (Edited by P. Grunwald), Pan Stanford Publishing, 115-159.
[10] Theerachat M., Emond S., Cambon E., Bordes F., Marty A., Nicaud J.-M., Chulalaksananukul W., Guieysse D., Remaud-Siméon M., Morel S. (2012). Engineering and production of laccase from Trametes versicolor in the yeast Yarrowia lipolytica. Bioresource Technology 125, 267–274. read online
[9] Emond S., Guieysse D., Lechevallier S., Dexpert-Ghys J., Monsan P., Remaud-Siméon M. (2012). Alteration of enzyme activity and enantioselectivity by biomimetic encapsulation in silica particles. Chemical Communications 48(9), 1314–1316. read online
[8] Emond S., Montanier C., Nicaud J.-M., Marty A., André I., Monsan P., Remaud-Siméon M. (2010). New Efficient Recombinant Expression System To Engineer Candida antarctica Lipase B. Applied and Environmental Microbiology 76(8), pp. 2684-2687. read online
[7] Mondon P., Grand D., Souyris N., Emond S., Bouayadi K., Kharrat H. (2010). MutaGenTM, a random mutagenesis method providing a complementary diversity generated by human error-prone DNA polymerases. Methods in Molecular Biology 634, 373-386. read online
[6] Emond S., Mondeil S., Jaziri K., André I., Monsan P., Remaud-Siméon M., Potocki-Véronèse G. (2008). Cloning, purification and characterization of a thermostable amylosucrase from Deinococcus geothermalis. FEMS Microbiology Letters, 285(1), pp. 25-32. read online
[5] Emond S., André I., Jaziri K., Potocki-Véronèse G., Mondon P., Bouayadi K., Kharrat H., Monsan P., Remaud-Siméon M. (2008). Combinatorial engineering to enhance amylosucrase thermostability. Protein Science, 17(6), pp. 967-976. read online
[4] Emond S., Mondon P., Pizzut-Serin S., Douchy L., Crozet F., Bouayadi K., Kharrat H., Potocki-Véronèse G., Monsan P., Remaud-Siméon M. (2008). A novel random mutagenesis approach using human mutagenic DNA polymerases to generate enzyme variant libraries. Protein Engineering, Design and Selection, 21(4), pp. 267-274. read online
[3] Emond S., Potocki-Véronèse G., Mondon P., Bouayadi K., Kharrat H., Monsan P., Remaud-Siméon M. (2007). Optimized and Automated Protocols for High-Throughput Screening of Amylosucrase Libraries. Journal of Biomolecular Screening, 12(5), pp. 715-723. read online
[2] Fabre E., Joucla G., Moulis C., Emond S., Richard G., Potocki-Véronèse G., Monsan P., Remaud-Siméon M. (2006). Glucansucrases from family 70 of the Glycoside-Hydrolases: What are the determinants of their specifities? Biocatalysis and Biotransformation, 24(1-2), pp. 137-145. read online
[1] Lesca C., Germanier M., Raynaud-Messina B., Pichereaux C., Etievant C., Emond S., Burlet-Schiltz O., Monsarrat B., Wright M., Defais M. (2005). DNA damage induce gamma-tubulin-RAD51 nuclear complexes in mammalian cells. Oncogene, 24(33), pp. 5165-5172. read online

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