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Hollfelder group

Laurens Lindenburg

Joined the lab: July 2014
Background and Current Projects: After completing an MSc in Biomolecular Sciences at Vrije Universiteit Amsterdam in 2009 I moved south to carry out a PhD in the laboratory for Chemical Biology in Eindhoven University of Technology (2009-2014). Under the guidance of Dr. Maarten Merkx, I developed fluorescent protein-based sensors to measure metal ion concentrations and metabolites in the mammalian cell.
As a postdoc in the Hollfelder group, I am developing several fluorescence-based screening assays to support directed evolution campaigns. I am interested in using directed evolution to probe the mechanisms of specificity in mammalian protein kinases. Another goal is to develop novel platforms for the directed evolution of enzymes for industrial biocatalysis.

Selected publications:

[1] Lindenburg LH, Malisauskas M, Sips T, van Oppen L, Wijnands SP, van de Graaf SF, Merkx M (2014). Quantifying stickiness: thermodynamic characterization of intramolecular domain interactions to guide the design of förster resonance energy transfer sensors. Biochemistry, 3: 6370.

[2] Lindenburg LH, Vinkenborg JL, Oortwijn J, Aper SJ, Merkx M (2013). MagFRET: the first genetically encoded fluorescent Mg2+ sensor. PLoS One, 8: e82009.

[3] Lindenburg LH, Hessels AM, Ebberink EH, Arts R, Merkx M (2013). Robust red FRET sensors using self-associating fluorescent domains. ACS Chemical Biology, 8: 2133.

[4] Drevinek P, Baldwin A, Lindenburg L, Joshi LT, Marchbank A, Vosahlikova S, Dowson CG, Mahenthiralingam E (2010). Oxidative stress of Burkholderia cenocepacia induces insertion sequence-mediated genomic rearrangements that interfere with macrorestriction-based genotyping. Journal of Clinical Microbiology, 48: 34.

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