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Anastasia Zinchenko

  • PhD student
  • Group of Florian Hollfelder
  • Department of Biochemistry, University of Cambridge, UK
  • Contact email: az305 (please add

Background: I studied Chemistry at Saarland University and Biosciences at the University of Exeter. Then I joined the group of Asst. Prof. William Clemons at the California Institute of Technology to research on bacterial N-linked glycosylation for my diploma thesis.

Training and Transferable Skills:
- Postgraduate Training Course in Biochemistry Techniques 2011
- Graduate School of Life Sciences Poster and Image Competitions
- Creative Research Workshop

Research Projects: I am working on the development of high-throughput screening using microfluidic devices to evolve enzymes that cleave phosphates. High-throughput screening in microdroplets possesses a number of advantages, such as parallel performance of millions of reaction, low reagent consumption and fast selection of positive hits by fluorescence activated cell sorting (FACS) or on-chip. In contrast to other technologies, such as display methods, this system is able to assess the catalytic function of a biocatalyst when large libraries are screened (up to 109 members) by maintaining not only the genotype-phenotype linkage but also trapping the product as detector molecule in the droplet. The system developed in this project aims to establish the first quantitative, fluorescence activated cell sorting (FACS) sortable, in vitro compartmentalized high-throughput system for directed evolution of enzymes, such as phosphatases.