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NextSeq500

The NextSeq 500 Sequencing System delivers the power of high-throughput sequencing with the speed, simplicity, and affordability of a desktop NGS (next-generation sequencing) system. The fast, integrated, sample-to-results workflow enables many sequencing applications—including exomes, whole genomes, and transcriptomes—in a single run.

NextSeq500 Performance
Flow Cell Read Length (bp) Output (Gb) Run Time
High Output

300 cycle kit

(2 x 150)

100 - 120 29 hrs

150 cycle kit

(2 x 75)

50 -60 18 hrs

75 cycle kit

(1 x 75)

25 - 30 11 hrs
Mid Output

300 cycle kit

(2 x 150)

32 - 39 26 hrs

150 cycle kit

(2 x 75)

16 - 19

15 hrs

Flow CellSingle ReadsPaired-End Reads
High Output up to 400 million up to 800 million
Mid Output up to 130 million up to 260 million

Data quality >75% higher than Q30 at 2 x 150 bp