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Illumina Sample Requirements

Customer Prepared Samples

If you have prepared your own Illumina compatible sequencing libraries, then we require at least 10 nM sample DNA in 20 μl.
If you require sample de-multiplexing please provide us with the index sequences used.

To convert ng/μl DNA concentration to nM
[nM DNA] = DNA concentration (ng/μl) x 1e6 (μl/L) / (Sample fragment size in bp x 656.4 (g/mole))

Using Custom Primers

It is possible to use custom sequencing primers (Custom Primer for Read 1, Custom Primer for Read 2 or Custom Primer for the Index Read) for a run on the MiSeq and NextSeq by loading custom primers into the reagent cartridge.
You can also use a combination of custom primers and Illumina primers provided in the reagent cartridge. For example, you can use the Illumina primer for Read 1, a custom primer for the Index Read and the Illumina primer for Read 2.
For the MiSeq we require at least 600 μl of the custom primer at 0.5 μM. For the NextSeq we require at least 2.5 ml of the custom primer at 0.5 μM.