Customer Prepared Samples
If you have prepared your own Illumina compatible sequencing libraries, then we require at least 10 nM sample DNA in 20 μl.
If you require sample de-multiplexing please provide us with the index sequences used.
To convert ng/μl DNA concentration to nM
[nM DNA] = DNA concentration (ng/μl) x 1e6 (μl/L) / (Sample fragment size in bp x 656.4 (g/mole))
Using Custom Primers
It is possible to use custom sequencing primers (Custom Primer for Read 1, Custom Primer for Read 2 or Custom Primer for the Index Read) for a run on the MiSeq and NextSeq by loading custom primers into the reagent cartridge.
You can also use a combination of custom primers and Illumina primers provided in the reagent cartridge. For example, you can use the Illumina primer for Read 1, a custom primer for the Index Read and the Illumina primer for Read 2.
We require at least 600 μl of the custom primer at 0.5 μM.