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MiSeq

Information courtesy of Illumina

The Illumina MiSeq System combines proven sequencing by synthesis (SBS) technology with a workflow that enables you to go from DNA to analyzed data in as little as eight hours. The MiSeq integrates cluster amplification, sequencing and data analysis in a single instrument.

MiSeq Performance
Total time Output
Read Length Current Current
50 cycle kit (2 x 25 bp) V2 5.5 hrs 750 - 850 Mb
150 cycle kit (2 x 75 bp) V3 20 hrs 3.3 - 3.8 Gb
300 cycle kit (2 x 150 bp) v2 Nano 300 Mb
300 cycle kit (2 x 150 bp) v2 Micro 1.2 Gb
300 cycle kit (2 x 150 bp) V2 24 hrs 4.5 - 5.1 Gb
500 cycle kit (2 x 250 bp) V2 Nano 500 Mb
500 cycle kit (2 x 250 bp) V2 39 hrs 7.5 - 8.5 Gb
600 cycle kit (2 x 300 bp) V3 55 hrs 13.2 - 15 Gb
Reads Passing Filter V2 Chemistry V3 Chemistry
Single Reads

15 - 17 million

Micro 4 million

Nano 1 million

22 - 25 million
Paired-End Reads

30 - 34 million

Micro 8 million

Nano 2 million

44 - 50 million
Quality Scores
1 x 36 bp > 90% bases higher than Q30
2 x 25 bp > 90% bases higher than Q30
2 x 75 bp > 85% bases higher than Q30
2 x 150 bp > 80% bases higher than Q30
2 x 250 bp > 75% bases higher than Q30
2 x 300 bp > 70% bases higher than Q30