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Vacancies in the Department

AZ CASE 4 year PhD Studentship (Fixed Term)

From Jobs at the University of Cambridge. Published on Mar 29, 2017.

Development of novel mass spectrometry approaches that enable phosphoproteomic analysis in tandem with cellular localisation

Department name: Cambridge Centre for Proteomics (CCP), University of Cambridge (http://proteomics.bio.cam.ac.uk/) Supervisors: Primary: Professor Kathryn Lilley (University of Cambridge) and Derek Barratt (https://www.astrazeneca.co.uk/)

As a key mediator of cellular signalling, phosphorylation remains a principal target for biological question. Identifying and quantifying the phosphorylation state of proteins involved in cell progression, metabolism, growth and disease is critical for the elucidation of cellular function. The initial aim of the project with be to extend recent developments that have demonstrated that the combination of phosphopeptides enrichment using titanium matrices and isobaric chemical labelling (TMT) allows the identification of 10,000 to 15,000 phosphopeptides from total cell lysate (1, 2). This coupled with the hyperLOPIT methodology (Localisation of Organelle proteins by isotope tagging, 3), which combines a biochemical organelle fractionation with a quantitative mass spectrometry analysis, will enable proteomic analysis at the organelle level. Whilst scientifically and experimentally challenging it is realistic to envisage the combination of these methods to deliver a novel approach which will add cellular resolution to phosphoproteomic analysis.

The second goal of the research will be the application of these methods to probe disease relevant cell types and response to compound treatment. This will leverage AstraZeneca's disease expertise and wealth of annotated probe compounds. Hypotheses generated from these analyses with be further tested by, targeted proteomic strategies (Selected Reaction Monitoring) and antibody methods to directly characterise the phosphorylation state of the protein candidates and their localisation within the cell using Western blotting and fluorescence microscopy. Finally, exploiting AstraZeneca's access to disease relevant tissues the expression of targeted protein candidates can be validated on mouse/human samples.

The successful candidate will be jointly supervised by Prof. Kathryn Lilley of the Cambridge Centre for Proteomics which is located in the Department of Biochemistry, University of Cambridge and Discovery Sciences Labs at the Darwin Building on the Cambridge Science Park, working alongside a team delivering Mechanistic Biology support to Oncology Drug Discovery Projects.

Applications

Applications should be sent to Prof. Kathryn Lilley (k.s.lilley@bioc.cam.ac.uk), by midnight on Friday 21st April 2017. enclosing a cover letter, a detailed curriculum vitae (both in PDF format) and the names and contact details of two academic referees.

Funding Notes

This studentship covers 4 years' UK/EU tuition fees (see below for EU eligibility requirements) and a maintenance stipend. BBSRC funding is available for UK nationals and EU students who meet the residency requirements. Further information about eligibility for funding can be found on the BBSRC website: http://www.bbsrc.ac.uk/documents/studentship-eligibility-pdf/

References (1) Evaluating multiplexed quantitative phosphopeptide analysis on a hybrid quadrupole mass filter/linear ion trap/orbitrap mass spectrometer. Erickson BK, Jedrychowski MP, McAlister GC, Everley RA, Kunz R, Gygi SP. Anal Chem. 2015. (2) Comprehensive quantitative comparison of the membrane proteome, phosphoproteome, and sialiome of human embryonic and neural stem cells. Melo-Braga MN, Schulz M, Liu Q, Swistowski A, Palmisano G, Engholm-Keller K, Jakobsen L, Zeng X, Larsen MR. Mol Cell Proteomics. 2014. (3) A draft map of the mouse pluripotent stem cell spatial proteome. Christoforou A, Mulvey CM, Breckels LM, Geladaki A, Hurrell T, Hayward PC, Naake T, Gatto L, Viner R, Martinez Arias A, Lilley KS. Nat Commun. 2016.

Fixed-term: The funds for this post are available for 4 years.

Please quote reference PH11837 on your application and in any correspondence about this vacancy.

The University values diversity and is committed to equality of opportunity.

The University has a responsibility to ensure that all employees are eligible to live and work in the UK.

Building Services Technician

From Jobs at the University of Cambridge. Published on Mar 17, 2017.

THIS IS A RE-ADVERTISEMENT. PREVIOUS UNSUCCESSFUL APPLICANTS NEED NOT APPLY.

A Building Service Technician (Plumbing Lead) is required to work in the University of Cambridge, Department of Biochemistry.

The successful applicant will join a small proactive team of three staff under the guidance of the Building Services Manager. The duties of the role include to support both teaching and research within The Department by providing a frontline service. Further duties of the role will be to monitor key plant and equipment, respond to breakdowns, conduct minor plumbing and building repairs / alterations, establish and carry out a range of routine and planned maintenance and minor building works as directed. Since an additional key part of this role will be to act as the Plumbing Lead within The Maintenance Team, the successful candidate will be competent and knowledgeable in the operation of general water, Air Handling Units and heating plant and the setting of control equipment associated with domestic, laboratory, softened and deionised water services.

One of the largest Department's in The School of Biological Sciences, Biochemistry has varied research activities using complex plant and services to maintain a workable environment for researchers and equipment. The Department occupies two main buildings; both being highly serviced by a large amount of plant equipment supporting a number of high-specification Laboratories. The successful Candidate will be expected to familiarise themselves with the numerous plant rooms in order to guide engineers and contractors when required.

The successful applicant will be able to work in a team, be computer literate and with a good attention to detail. Communication and interpersonal skills are essential as the role holder is required to deal with different personnel both from within The University and outside contractors on a daily basis.

Candidates should hold an industry recognised qualification (eg., BTEC National certificate, City & Guilds, NVQ or equivalent).

Once an offer of employment has been accepted, the successful candidate will be required to undergo a health assessment.

Enquires relating to this role should be directed to Roger Biamonti (rb686@cam.ac.uk).

To apply online for this vacancy, please click on the 'Apply' button below. This will route you to the University's Web Recruitment System, where you will need to register an account (if you have not already) and log in before completing the online application form.

Any enquires regarding the application process can be directed to Adriana Dote (personnel@bioc.cam.ac.uk).

Please quote reference PH11380 on your application and in any correspondence about this vacancy.

The University values diversity and is committed to equality of opportunity.

The University has a responsibility to ensure that all employees are eligible to live and work in the UK.

PhD studentship in Molecular Microbiology

From Jobs at the University of Cambridge. Published on Feb 10, 2017.

A BBSRC-funded Targeted PhD studentship (for uptake in October 2017) is available in the laboratory of Dr Martin Welch (Department of Biochemistry, Tennis Court Road, Cambridge). The project will investigate the role of an acetate-inducible denitrification pathway in the opportunistic human pathogen, Pseudomonas aeruginosa. Recent RNA-Seq analyses carried out in the lab have revealed that when grown on acetate as a sole carbon source, gene products comprising the Nar/Nir/Nos/Nor denitrification pathways are among the most highly up-regulated transcripts in the cell. This was a surprising observation because transcripts encoding the known regulators of denitrification in P. aeruginosa (Anr and Dnr) were not modulated at all, suggesting that in these conditions, denitrification is controlled by some other mechanism(s). The practical relevance of this is that (i) fatty acids and other acetate-producing substrates are now known to be the preferred energy source for P. aeruginosa during many infection scenarios, and (ii) denitrification is critically-important in industry for microaerobic bacterial growth. Therefore, the key question and the main aim of this project, is to understand why P. aeruginosa turns on the denitrification pathways during growth on acetate, and what regulates this. The successful candidate will investigate this problem using a combined biochemical, genetic and systems-based approach which should provide a rich and varied training opportunity.

The successful applicant will join our Departmental PhD programme and must complete their studies within 4 years. Full funding (covering maintenance, fees and laboratory consumables) is available for UK citizens or EEA students who meet UK residency requirements. In addition to a programme of work in the MW lab, the appointed student will participate in DTP training courses, two 10-week rotation projects (one of which will be based in the MW lab) and a three month internship (PIPS), as well as other DTP-related events.

In the first instance, interested candidates should contact Dr Martin Welch (mw240@cam.ac.uk) with a CV, list of academic referees and a cover letter. Shortlisted candidates will be invited for interview in Cambridge.

Please quote reference PH11425 on your application and in any correspondence about this vacancy.

The University values diversity and is committed to equality of opportunity.

The University has a responsibility to ensure that all employees are eligible to live and work in the UK.

Research Associate

From Jobs at the University of Cambridge. Published on Mar 23, 2017.

Postdoctoral Research Associate - biotechnological exploitation of photosynthetic microorganisms

The project aims to study the use of photosynthetic microorganisms and macroalgae for the remediation of waste materials.

Applicants should have well-established experience in the biochemistry, growth and genetic manipulation of microorganisms, including photosynthetic species. They should also have well-established experience in routine molecular biological techniques (such as generation of constructs for microbial genetic manipulation). Experience in the biology of macro-algae and/or the bioinformatic analysis of transcriptomic data would also be desirable, but not essential.

The project is a collaborative one between the laboratories of Prof C J Howe in the Department of Biochemistry and Prof J S Dennis in the Department of Chemical Engineering and Biotechnology.

The post is available immediately.

Limited funding: The funds for this post are available for 11 months in the first instance.

To apply online for this vacancy, please click on the 'Apply' button below. This will route you to the University's Web Recruitment System, where you will need to register an account (if you have not already) and log in before completing the online application form.

Any enquires regarding the application process can be directed to Adriana Dote (personnel@bioc.cam.ac.uk).

Informal enquiries may be made to Prof C J Howe (c.j.howe@bioc.cam.ac.uk).

Please quote reference PH11775 on your application and in any correspondence about this vacancy.

The University values diversity and is committed to equality of opportunity.

The University has a responsibility to ensure that all employees are eligible to live and work in the UK.

Research Associate (Fixed Term)

From Jobs at the University of Cambridge. Published on Mar 23, 2017.

Applications are invited to recruit an enthusiastic post-doctoral scientist on a BBSRC funded research project, investigating infant lipid metabolism with the aim to identify robust lipidomic markers which reflect healthy growth and development. The project is led by Dr Albert Koulman (director of the NIHR BRC Core Metabolomics and Lipidomics Laboratory and the NIHR BRC Nutritional Biomarker laboratory), in close collaboration with the department of Paediatrics (Prof David Dunger) and King's College London (Dr Sophie Moore).

The overall aim of the project if develop and establish lipid profiling from dried blood spots as biomarkers, which will therefore deliver a robust indicator of metabolic efficacy of infant nutrition and will thus provide a practical tool that can assist the study of feeding strategies and development of new infant formulae for infants that cannot or should not be fully breastfed. The project is part of a larger effort to understand how lipid metabolism is influenced by infant nutrition and contributes to metabolic programming.

Applications are invited from researchers with a PhD, and a proven track record in working with metabolomics or lipidomics data (evidence of one or more high impact publications in a relevant research field). Previous experience with research in paediatrics or infant growth and development is desired. Applicants should have excellent interpersonal and communication skills, and the ability to work as part of a team as well as independently when required.

Fixed-term: The funds for this post are available for 2 and half years in the first instance.

To apply online for this vacancy, please click on the 'Apply' button below. This will route you to the University's Web Recruitment System, where you will need to register an account (if you have not already) and log in before completing the online application form.

Any enquires regarding the application process can be directed to Adriana Dote (personnel@bioc.cam.ac.uk).

Informal enquiries can be sent to Dr Albert Koulman (ak675@medschl.cam.ac.uk).

Interviews will be taking place towards the end of April 2017.

Please quote reference PH11774 on your application and in any correspondence about this vacancy.

The University values diversity and is committed to equality of opportunity.

The University has a responsibility to ensure that all employees are eligible to live and work in the UK.

Research Associate (Fixed Term)

From Jobs at the University of Cambridge. Published on Mar 22, 2017.

Structural studies of mosquito vector immunity

A three-year postdoctoral position for a biochemist/structural biologist is available in the Department of Biochemistry, University of Cambridge, England. The Department has state-of-the-art equipment and facilities, including crystallization robots, NMR, cryo-EM, biophysics, metabolomics, and easy access to synchrotron beamlines and confocal microscopy. The postdoctoral position is available immediately with Dr Monique Gangloff following a New Investigator Research Grant award to study mosquito vector innate immunity.

We are looking for a highly motivated postdoctoral Fellow with a strong interest in Structural Biology, Signalling and Vector Biology. The project investigates the structure and function of mosquito immune receptors that have diverged and undergone gene duplication under the influence of selective pressure by pathogenic parasites and viruses, potentially acquiring new functions relating to host-pathogen homeostasis.

You will gain in-depth experience in Structural Biology by determining receptor-ligand complex structures using either X-ray crystallography or complementary techniques such as Small Angle X-ray Scattering (SAXS) and high-resolution cryo-EM. You will establish cell-based assays to verify predicted structure-function relationships. It is expected that you present your results at scientific conferences and write/publish scientific papers in high-quality, peer-reviewed journals.

Essential requirements:

  • PhD in biochemistry, molecular and cellular biology, or related disciplines

  • Strong background in construct design, molecular biology and protein purification

  • Significant experience with biophysical and structural techniques

  • Strong organizational and interpersonal skills

  • A proven record of work published in reputable scientific journals.

Desirable requirements:

  • Experience of protein expression methods using prokaryotic and eukaryotic in particular insect cell expression systems

  • Proficiency in cell-based signaling assays to establish novel luciferase reporter assays in mosquito cell lines

  • Knowledge in ligand fishing experiments using Mass Spectrometry

  • Experience of working with Protein Crystallization, X-ray crystallography, SAXS, cryo-EM, Isothermal Titration Calorimetry and Surface Plasmon Resonance.

To apply online for this vacancy, please click on the 'Apply' button below. This will route you to the University's Web Recruitment System, where you will need to register an account (if you have not already) and log in before completing the online application form.

The application should include a cover letter describing the candidate's research interests and motivation to apply, the CV and list of publications, and contact details of three referees.

Any enquires regarding the application process can be directed to Adriana Dote (personnel@bioc.cam.ac.uk).

Informal enquiries are welcome and can be made to Dr. Monique Gangloff (mg308@cam.ac.uk).

Please quote reference PH11763 on your application and in any correspondence about this vacancy.

The University values diversity and is committed to equality of opportunity.

The University has a responsibility to ensure that all employees are eligible to live and work in the UK.