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Vacancies in the Department

BBSRC PhD Studentship

From Jobs at the University of Cambridge. Published on Jan 05, 2015.

A BBSRC PhD studentship fully funded for four years is available in the laboratory of Professor Paul Dupree. The project is in partnership with Novozymes, the world's largest enzyme discovery and production company.

The industrial collaborative project aims to develop tools and understanding with application to the bioenergy, biorefining and dietary uses of plants. The project goal is to understand the role of the plant cell wall polysaccharide xylan in preventing enzymatic digestion of cellulose microfibrils. Our recent discoveries propose new models for xylan interaction with cellulose (Busse Wicher et al. 2014). The new models suggest new types of enzymes may be involved in plant cell wall deconstruction. The project will use the extensive range of specific recombinant hydrolases and polysaccharide oxidases (e.g. cellulases, xylanases, LPMOs) available from Novozymes, and also the extensive panel of plants with modified plant cell wall synthesis available in the laboratory of Paul Dupree.

The student will spend at least three months in Novozymes, Copenhagen, gaining experience of industrial enzyme discovery and development.

The project will start during the 2015-2016 academic year.

Busse-Wicher M et al. (2014) The pattern of xylan acetylation suggests xylan may interact with cellulose microfibrils as a two-fold helical screw in the secondary plant cell wall of Arabidopsis thaliana. Plant J. 79(3):492-506.

Please contact Professor Paul Dupree - pd101@cam.ac.uk for any enquiries.

BBSRC studentship eligibility conditions apply.

http://www.bioenergy.novozymes.com

Please quote reference PH04924 on your application and in any correspondence about this vacancy.

The University values diversity and is committed to equality of opportunity.

The University has a responsibility to ensure that all employees are eligible to live and work in the UK.

Marie-Sklodowska-Curie ITN PhD Studentship

From Jobs at the University of Cambridge. Published on Mar 03, 2015.

A 3-year EU-funded PhD studentship is available in the laboratory of Dr Martin Welch. Applications are invited for a PhD studentship position ("Early Stage Researcher" or ESR) to be funded by the Marie-Sklodowska-Curie Innovative Training Network "INTEGRATE ¿ Interdisciplinary Training Network for Validation of Gram-Negative Antibacterial Targets" within the Horizon 2020 Programme of the European Commission. Researchers are required to undertake trans-national mobility (i.e., move from one country to another) when taking up the appointment.

Project Title: The glyoxylate shunt as a target for antibacterial intervention in Gram-negative bacteria.

The junction between the TCA cycle and the glyoxylate shunt is widely regarded as one of the most important metabolic branchpoints in the whole of microbial metabolism, and is increasingly being recognized as a major new target for the development of antibacterial compounds (Munoz-Elias & MacKinney, Nature Medicine 11, 638 - 44 (2005) & Sharma et al. Nat Struct Biol. 7(8):663-8 (2000)). In addition, carbon flux through the glyoxylate shunt plays a central role in regulating virulence in the Gram-negative "superbug", Pseudomonas aeruginosa (Chung et al, Open Biology (2013)). Indeed, mutants defective in isocitrate lyase (ICL; the first enzyme of the glyoxylate shunt) display impaired fitness in vivo. Consequently, compounds that target the enzymes of the glyoxylate shunt, or which reduce the flux of carbon directed through this pathway, may have the dual effect of reducing tissue damage (by blocking virulence) and diminishing the metabolic fitness of the organism in the niche. However, the branchpoint between the TCA cycle and glyoxylate shunt in P. aeruginosa differs markedly from the canonical organisation in E. coli, and nothing is known about its enzymology. The aim of this PhD project is to purify the enzymes involved, and to characterize these in considerable detail as potential targets for antimicrobial intervention. The kinetic parameters of each P. aeruginosa branchpoint enzyme (which includes two isocitrate dehydrogenases (ICD and IDH), a kinase/phosphatase (AceK) and ICL) will be determined, as will the impact of putative allosteric regulators. HTP screening of compound libraries (including natural product and Diversity oriented synthesis (DoS) libraries, available in-house) will be carried out to identify inhibitors/activators of each enzyme. We will characterize the nature of the inhibition/activation mechanisms (competitive, non-competitive, etc.) and attempt to co-crystallize these compounds as complexes bound to their cognate target enzymes.

The appointed ESR will spend at three months seconded to Aptuit (Verona, Italy) and five months seconded with the University of Helsinki (Finland) to allow them to learn and apply new techniques.

Shortlisted candidates will be invited the INTEGRATE Recruitment Event in Verona (Italy) on 5-6 May 2015. The Recruitment Committee will subsequently interview each candidate. In order to cover (part of) your travel and lodging costs, shortlisted candidates will receive a lump sum of €250.

The project will start during the 2015-2016 academic year.

An online application form is available by clicking;

http://www.cdr.fi/news-and-events/integrate-etn-student-application-form

The deadline for applications routed through the above website is March 9th, but Dr welch will still consider applications from exceptional individuals until the end of April. If you wish to apply for this position after March 9th, or if you have any queries regarding the position, please contact Dr Welch directly - mw240@cam.ac.uk .The Welch lab website can be found at: http://www.bioc.cam.ac.uk/people/uto/welch

Please quote reference PH05532 on your application and in any correspondence about this vacancy.

The University values diversity and is committed to equality of opportunity.

The University has a responsibility to ensure that all employees are eligible to live and work in the UK.

Research Associate (Fixed Term)

From Jobs at the University of Cambridge. Published on Feb 18, 2015.

A postdoctoral research associate position is available for up to twelve months to work in the laboratory of Dr A.P. Jackson, University of Cambridge, Department of Biochemistry, Tennis Court Road, Cambridge. The project will study the role of sodium channel ß-subunits in the structure and assembly of the pain-sensing sodium channel Nav 1.7, and its potential for drug targeting.

We are looking for an experienced structural biologist, ideally with some background in ion channel structure and a familiarity with general molecular biological methods. The work is a collaborative project between Dr A.P. Jackson, Dr DY Chirgadze (Dept. Biochemistry), Prof. C-L. Huang (Dept. Physiology, Development and Neuroscience) and the Antibody Discovery and Protein Engineering Department, MedImmune. It builds on recent evidence that some ß-subunits can cross-link sodium channels together on the neuronal plasma membrane (see: Namadurai et al., Open Biol. 2015 5:140192. DOI: 10.1098/rsob.140192). The basis of this cross-linking, and the role of ß-subunits in modulating drug binding will be investigated using structural and functional methods.

Skills required in tissue culture and basic molecular biology, experience with X-ray crystallography would be an advantage.

Applicants should have a PhD or the equivalent in a relevant science.

The University values diversity and is committed to equality of opportunity.

Fixed-term: The funds for this post are available for up to 12 months in the first instance.

To apply online for this vacancy, please click on the 'Apply' button below. This will route you to the University's Web Recruitment System, where you will need to register an account (if you have not already) and log in before completing the online application form.

For all informal inquiries, please contact Dr A.P. Jackson - apj10@cam.ac.uk

Please quote reference PH05392 on your application and in any correspondence about this vacancy.

The University values diversity and is committed to equality of opportunity.

The University has a responsibility to ensure that all employees are eligible to live and work in the UK.

Research Associate [Temporary Cover] (Maternity Cover)

From Jobs at the University of Cambridge. Published on Mar 03, 2015.

A temporary postdoctoral research associate position is available for a period of 6 months to work in the laboratory of Professor Ernest Laue at the Department of Biochemistry, University of Cambridge, Tennis Court Road, Central Cambridge.

The Laue group is interested in the way chromatin structure controls epigenetic inheritance, and the study of proteins and complexes involved in chromatin assembly/disassembly. Our current research is focussed on studies of the Nucleosome Remodelling Deacetylase complex (NuRD), as part of the EU FP7 project '4DCellfate'. NuRD plays a key role in controlling the differentiation of embryonic stem (ES) cells and the reprogramming of adult cells to a pluripotent state.

We are looking for an experienced biochemist with expertise in the expression/purification of protein complexes. The project will involve expression of NuRD complexes in insect cells, e.g. using the MultiBac system. The project would also suit a structural biologist, ideally with some background in X-ray crystallography or Electron Microscopy (EM), who has good expertise in biochemical methods.

Applicants should have a PhD or equivalent in a relevant area of science.

Maternity cover: This post is fixed-term for 6 months or the return of the post holder, whichever is the earlier.

To apply online for this vacancy, please click on the 'Apply' button below. This will route you to the University's Web Recruitment System, where you will need to register an account (if you have not already) and log in before completing the online application form.

For all informal inquiries, please contact Professor Laue - e.d.laue@bioc.cam.ac.uk

Please quote reference PH05520 on your application and in any correspondence about this vacancy.

The University values diversity and is committed to equality of opportunity.

The University has a responsibility to ensure that all employees are eligible to live and work in the UK.

Research Data Technician (Fixed Term)

From Jobs at the University of Cambridge. Published on Feb 26, 2015.

Cambridge Centre for Proteomics (CCP) is currently looking to recruit a Research Data Technician within its core facility unit. The core facility offers proteomics services to users from the University of Cambridge and other worldwide academic and industrial groups.

The successful candidate will be required to analyse proteomics data using standard proteomic packages including Proteome Discoverer, Mascot and Scaffold and apply common statistical methods to datasets. Some degree of programming literacy is essential, preferably R/Bioconductor. The role-holder will be an integral member of CCP and will need to interact closely with other members of CCP and internal and external users of the service to provide interpretation of results from complex datasets.

The successful candidate will have a first degree in computer or biological sciences or statistics and previous experience in working with high throughput mass spectrometry data sets would be advantageous.

Fixed-term: The funds for this post are available for 2 years in the first instance.

Once an offer of employment has been accepted, the successful candidate will be required to undergo a health assessment.

To apply online for this vacancy, please click on the 'Apply' button below. This will route you to the University's Web Recruitment System, where you will need to register an account (if you have not already) and log in before completing the online application form.

For all inquiries, please contact Mike Deery, md289@cam.ac.uk.

Please quote reference PH05266 on your application and in any correspondence about this vacancy.

The University values diversity and is committed to equality of opportunity.

The University has a responsibility to ensure that all employees are eligible to live and work in the UK.