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Vacancies in the Department

Two EU Marie-Curie PhD Studentships in collaboration with MedImmune: Protease-activated and protease-based protein drugs(Fixed Term)

From Jobs at the University of Cambridge. Published on Aug 18, 2016.

Medimmune and Department of Biochemistry, University of Cambridge

A graduate studentship leading to the award of a PhD is available from 1 March 2017 (or later) to work at MedImmune (with Dr Lutz Jermutus - https://www.researchgate.net/profile/Lutz_Jermutus) and at Cambridge University in the Biochemistry Department (with Dr. F. Hollfelder - http://www.bioc.cam.ac.uk/hollfelder). The studentship is in the context of the EU Marie-Curie network ES-Cat (http://www.bioc.cam.ac.uk/hollfelder/Research/es-cat) on Directed Protein Evolution for Synthetic Biology and Biocatalysis.

The objective of the project is to use protease promiscuity to design novel therapeutics. The first part of the project will look at engineering proteins that are selectively activated by proteases in a tissue of choice. For this, we will initially identify and characterize kidney- and heart-selective proteases through genetics and proteomics screens. We will then construct both monoclonal antibodies and therapeutic proteins that are tethered to an inhibitory peptide via a tissue-specific, protease-sensitive peptide linker and study their biodistribution in rodents. We will finally assess the impact of these modifications on antibody and protein efficacy in models of kidney or cardiac disease. The second part will build on novel protease-based drugs that are the focus of an on-going collaboration between the Hollfelder and Jermutus groups. This approach combines a protease activity with a substrate binding moiety as well as domain that will increase plasma half-life. We will look at variations of this yet unpublished idea of 'captivases' that will allow for a modular drug discovery platform.

Candidates will be mainly based in the Biochemistry Department (in central Cambridge) and the MedImmune/AstraZeneca laboratories (currently on Granta Park and relocating in 2017 to the Cambridge Biomedical Campus), but also work for extended secondments in the labs of the academic and industrial network partners (http://www.bioc.cam.ac.uk/hollfelder/Research/es-cat/es-catfront#partnerlist). All students take part in a training programme that includes workshops, meetings and courses.

Applicants should have a first (or upper second) class degree in biochemistry, biotechnology, chemistry or a related, relevant subject. Specific skills in protein engineering, directed evolution, chemical and kinetic analysis of enzyme mechanisms and familiarity with molecular biology techniques (e.g. phage or ribosome display, cloning, DNA manipulation, preparation and handling of DNA libraries) are advantages.

The full stipend will be paid at rates of the EU Marie-Curie programme for the 3 year duration of the award (€44896.96 per annum) and mobility allowance (€7200).

Fixed-term: The funds for this post are available for 36 months in the first instance.

Applications should contain a description of your research interests (e.g. as part of the cover letter) and your detailed CV (with exam marks and all relevant practical experience). Please also include the names and email-addresses of at least two referees, who know you from your courses or laboratory research work.

Send your application by 19 September 2016 by email to studenships@bioc.cam.ac.uk

Please quote reference PH09909 on your application and in any correspondence about this vacancy.

The University values diversity and is committed to equality of opportunity.

The University has a responsibility to ensure that all employees are eligible to live and work in the UK.

EU Marie-Curie PhD Studentship: Directed Protein Evolution for Synthetic Biology and Biocatalysis (Fixed Term)

From Jobs at the University of Cambridge. Published on Aug 18, 2016.

A graduate studentship leading to the award of a PhD is available from 1 March 2017 (or later) to work at Cambridge University in the Biochemistry Department (with Dr. F. Hollfelder - http://www.bioc.cam.ac.uk/hollfelder) and at the Biocatalysis Research Center of Johnsson Matthey. The studentship is in the context of the EU Marie-Curie network ES-Cat (http://www.bioc.cam.ac.uk/hollfelder/Research/es-cat) on Directed Protein Evolution for Synthetic Biology and Biocatalysis.

The objective of the project is the directed evolution of enzymes for chiral chemo-enzymatic synthesis of industrially relevant enzymes. The use of the most powerful ultrahigh throughput format available, microfluidic droplets, will make selections from libraries with more than a million members possible. In addition to man-made randomized protein libraries, we will also select from metagenomic libraries. Kinetic and structural analysis will help to delineate the chemical and evolutionary mechanisms that allow the emergence of new and improved activities.

Candidates will be based in Cambridge working in the Biochemistry Department of Cambridge University and also at Johnsson Matthey in the Cambridge Sceince Park. Extended secondments in the labs of the academic and industrial network partners (http://www.bioc.cam.ac.uk/hollfelder/Research/es-cat/es-catfront#partnerlist) are also envisaged. All students take part in a training programme that includes workshops, meetings and courses.

Applicants should have a first (or upper second) class degree in biochemistry, biotechnology, chemistry or a related, relevant subject. Specific skills in protein engineering, directed evolution, enzymology, biocatalysis, organic synthesis and familiarity with molecular biology techniques (e.g. cloning, DNA manipulation, preparation and handling of DNA libraries) are advantages.

The full stipend will be paid at rates of the EU Marie-Curie programme for the 3 year duration of the award (€44896.96 per annum) and mobility allowance (€7200).

Fixed-term: The funds for this post are available for 36 months in the first instance.

Applications should contain a description of your research interests (e.g. as part of the cover letter) and your detailed CV (with exam marks and all relevant practical experience). Please also include the names and email-addresses of at least two referees, who know you from your courses or laboratory research work.

Send your application by 10 September 2016 by email to studenships@bioc.cam.ac.uk.

Please quote reference PH09908 on your application and in any correspondence about this vacancy.

The University values diversity and is committed to equality of opportunity.

The University has a responsibility to ensure that all employees are eligible to live and work in the UK.

Research Associate (Fixed Term)

From Jobs at the University of Cambridge. Published on Aug 25, 2016.

A postdoctoral research associate position is available for 1 year in the first instance with the possibility of an extension to work in the laboratory of Prof. J.L. Griffin, Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge. The project will develop tools to measure lipid species in conjunction with ion mobility, an exciting development in mass spectrometry which will allow the separation of ions according to their cross sectional area as well as their mass to charge ratio. We are seeking a research associate with experience in biological mass spectrometry, and in particular the use of lipidomics, to study human disease processes.

We are looking for an experienced mass spectrometrist, ideally with some background in metabolomics and/or lipidomics. The work is a collaborative project between Prof. J.L Griffin (Department of Biochemistry) and Agilent Technology. As part of this project the research associates will have access to a state of the art ion mobility Q-ToF LC-MS/MS to develop new tools for lipidomics, and building on our work in diabetes and metabolic diseases use the instrument to explore cellular signalling in these disease processes. Some examples of this work can be found in Roberts LD et al., Lancet Endocrinology, 2014; Roberts LD et al., Methods in Enzymology, 2014; Sanders F, McNally B, Griffin JL. Blood triacylglycerols: a lipidomic window on diet and disease. Biochem Soc Trans. 2016 Apr 15;44(2):638-44.

The post requires skills in mass spectrometry, particularly applied to biological problems, and a general interest in metabolism and disease processes in mammals. Previous work in metabolomics, lipidomics and/or multivariate statistics would be an advantage.

Applicants should have a PhD or the equivalent in a relevant science.

Fixed-term: The funds for this post are available for up to 1 year in the first instance with the possibility the post will be renewed as part of this collaboration.

To apply online for this vacancy, please click on the 'Apply' button below. This will route you to the University's Web Recruitment System, where you will need to register an account (if you have not already) and log in before completing the online application form.

For all informal inquiries, please contact Prof. J.L Griffin - jules.griffin@mrc-hnr.cam.ac.uk.

Please quote reference PH09735 on your application and in any correspondence about this vacancy.

The University values diversity and is committed to equality of opportunity.

The University has a responsibility to ensure that all employees are eligible to live and work in the UK.

Research Grants Administrator - Maternity Cover or Secondment

From Jobs at the University of Cambridge. Published on Aug 17, 2016.

This position is for maternity cover - a secondment will be considered.

Applications are invited to provide maternity cover for the post of Research Grants Administrator. The successful application will be expected to manage a team of three staff who deliver grant financial services to a wide range of academic and research colleagues. You will have responsibility and oversight for all grant processes and income of approximately £50m including grant reconciliations.

The successful candidate will be used to working in an extremely busy environment whilst maintaining high levels of accuracy. You will be educated to degree level or equivalent, and hold professional qualifications to at least AAT Level 4 or have significant grant administration experience. In addition you will have worked in a grants environment with a strong customer service focus. Proven ability to resolve problems independently is essential. Knowledge of research grants, including MRC, BBSRC, UKRC and EU, and experience of their management is desirable.

Possible start date of mid October.

Secondment/Maternity cover: This post is fixed-term for 1 year or the return of the post holder, whichever is the earlier.

Once an offer of employment has been accepted, the successful candidate will be required to undergo a health assessment.

To apply online for this vacancy, please click on the 'Apply' button below. This will route you to the University's Web Recruitment System, where you will need to register an account (if you have not already) and log in before completing the online application form.

Informal enquiries can be directed to Steph Favrin - personnel@bioc.cam.ac.uk.

Please quote reference PH09901 on your application and in any correspondence about this vacancy.

The University values diversity and is committed to equality of opportunity.

The University has a responsibility to ensure that all employees are eligible to live and work in the UK.