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Vacancies in the Department

BBSRC PhD Studentship

From Jobs at the University of Cambridge. Published on Jan 05, 2015.

A BBSRC PhD studentship fully funded for four years is available in the laboratory of Professor Paul Dupree. The project is in partnership with Novozymes, the world's largest enzyme discovery and production company.

The industrial collaborative project aims to develop tools and understanding with application to the bioenergy, biorefining and dietary uses of plants. The project goal is to understand the role of the plant cell wall polysaccharide xylan in preventing enzymatic digestion of cellulose microfibrils. Our recent discoveries propose new models for xylan interaction with cellulose (Busse Wicher et al. 2014). The new models suggest new types of enzymes may be involved in plant cell wall deconstruction. The project will use the extensive range of specific recombinant hydrolases and polysaccharide oxidases (e.g. cellulases, xylanases, LPMOs) available from Novozymes, and also the extensive panel of plants with modified plant cell wall synthesis available in the laboratory of Paul Dupree.

The student will spend at least three months in Novozymes, Copenhagen, gaining experience of industrial enzyme discovery and development.

The project will start during the 2015-2016 academic year.

Busse-Wicher M et al. (2014) The pattern of xylan acetylation suggests xylan may interact with cellulose microfibrils as a two-fold helical screw in the secondary plant cell wall of Arabidopsis thaliana. Plant J. 79(3):492-506.

Please contact Professor Paul Dupree - pd101@cam.ac.uk for any enquiries.

BBSRC studentship eligibility conditions apply.

http://www.bioenergy.novozymes.com

Please quote reference PH04924 on your application and in any correspondence about this vacancy.

The University values diversity and is committed to equality of opportunity.

The University has a responsibility to ensure that all employees are eligible to live and work in the UK.

PhD Studentship (Fixed Term)

From Jobs at the University of Cambridge. Published on Jan 13, 2015.

PhD Studentship Department of Biochemistry, University of Cambridge

Single Cell Analysis in Microfluidic Droplets

A graduate studentship leading to the award of a PhD is available from 1 October 2015 (or later) to work at Cambridge University in the Biochemistry Department with Dr. F. Hollfelder (http://www.bioc.cam.ac.uk/hollfelder).

Single-cell studies promise to resolve ensemble measurements to give insight on cellular heterogeneity. Moving beyond averaging of populations this experimental approach provides more meaningful insight into the intrinsic cellular processes and the cellular responses to outside influences. We will study single cells compartmentalized in microfluidic droplets, thousands of cells can be studied by optical and transcriptomic analysis to identify interesting phenotypic characteristics, as a prelude to decipher molecular mechanisms, e.g. of cell diversification. The project will involve collaboration with Dr. Kevin Chalut (at the Stem Cell Institute - http://www.stemcells.cam.ac.uk/researchers/principal-investigators/kevin-chalut) and Dr. Sarah Teichmann (at the Department of Physics and the Euopean Bioinformatics Institute Single Cell Genomics Centre - http://www.ebi.ac.uk/~saraht/).

Applicants should have a first or upper second class degree in biochemistry, biotechnology, chemistry or a related, relevant subject. Specific skills in cell and molecular biology or, alternatively, microengineering techniques, microfluidics, soft lithography and applied physics are advantages.

This project is fully funded by the BBSRC for up to 48 months. Studentships are only available to citizens of the EU or the European Economic Area. To qualify for the full rate of the studentship UK residency requirements must be met (see e.g.

http://www.mrc.ac.uk/Fundingopportunities/Applicanthandbook/Studentships/Eligibility/index.htm#P17968 or http://www.bbsrc.ac.uk/web/FILES/Guidelines/studentshipeligibility.pdf).

The full stipend will be paid at RCUK rates for the 4 year duration of the award.

Fixed-term: The funds for this post are available for 4 years in the first instance.

Applications should contain a description of your research interests (e.g. as part of the cover letter) and your detailed CV (with exam marks and all relevant practical experience). Please also include the names and email-addresses of at least two referees, who know you from your courses or laboratory research work. Send your application by 20 February 2015 by email to fh111@cam.ac.uk.

Informal inquiries can be made by contacting Dr Florian Hollfelder - fh111@cam.ac.uk

Please quote reference PH05115 on your application and in any correspondence about this vacancy.

The University values diversity and is committed to equality of opportunity.

The University has a responsibility to ensure that all employees are eligible to live and work in the UK.

PhD Studentship: Protein Engineering by Phenotypic Screening in Microfluidic Droplets (Fixed Term)

From Jobs at the University of Cambridge. Published on Jan 13, 2015.

PhD Studentship in Chemical Biology and Protein Engineering Department of Biochemistry, University of Cambridge

Protein Engineering by Phenotypic Screening in Microfluidic Droplets

A graduate studentship leading to the award of a PhD is available from 1 October 2015 (or later) to work at Cambridge University in the Biochemistry Department (with Dr. F. Hollfelder - http://www.bioc.cam.ac.uk/hollfelder) and at MedImmune (with Dr. R. Minter - http://www.medimmune.com/).

Antibodies are usually raised against purified proteins, e.g. by affinity panning. In many cases this approach has led to valuable therapeutics, but good binders can also be therapeutically inactive, if the binding event is not linked to a biological effect. In a radical departure from conventional antibody screening, we will co-compartmentalise one antibody species with a single cell in pico- to nanolitre droplets that are generated at high speed (> >1kHz) in microfluidic devices. In this way we can screen millions of clones for their ability to alter cellular behaviour. Such an approach will be uniquely valuable to raise therapeutic molecules against targets that are relatively unstable, difficult to handle or cannot be produced in vitro, so that affinity panning is not an option. In the course of the studentship this new technology will be developed and applied and validated by addressing currently undruggable targets.

Candidates will be mainly based in the Biochemistry Department (in central Cambridge), but also work for extended secondments at MedImmune's Granta Park Site (near Cambridge) and/or in their US headquarters (Gaithersburg, Maryland) and thus receive training in a basic science as well as an industrial context.

Applicants should have a first or upper second class degree in biochemistry, biotechnology, chemistry or a related, relevant subject. Specific skills in protein engineering, directed evolution, cloning, DNA manipulation (including preparation and handling of DNA libraries) and familiarity with other molecular biology techniques (e.g. phage or ribosome display) are advantages.

This project is fully funded by the BBSRC (via a CASE award) for up to 48 months. Studentships are only available to citizens of the EU or the European Economic Area. To qualify for the full rate of the studentship UK residency requirements must be met (see e.g.

http://www.mrc.ac.uk/Fundingopportunities/Applicanthandbook/Studentships/Eligibility/index.htm#P17968 or http://www.bbsrc.ac.uk/web/FILES/Guidelines/studentshipeligibility.pdf).

The full stipend will be paid at RCUK rates for the 4 year duration.

Fixed-term: The funds for this post are available for 4 years in the first instance.

Applications should contain a description of your research interests (e.g. as part of the cover letter) and your detailed CV (with exam marks and all relevant practical experience). Please also include the names and email-addresses of at least two referees, who know you from your courses or laboratory research work. Send your application by 15 February 2015 by email to fh111@cam.ac.uk.

Informal inquiries can be made by contacting Dr Florian Hollfelder - fh111@cam.ac.uk

Please quote reference PH05109 on your application and in any correspondence about this vacancy.

The University values diversity and is committed to equality of opportunity.

The University has a responsibility to ensure that all employees are eligible to live and work in the UK.

Post-doctoral Research Associate (Fixed Term)

From Jobs at the University of Cambridge. Published on Jan 23, 2015.

A postdoctoral research associate position is available to work in the laboratory of Dr Tony Jackson based in the University of Cambridge, Department of Biochemistry, Tennis Court Road, Central Cambridge. The post is funded by the British Heart Foundation for up to three years. The project will study the sodium channel ß3-subunit, its role in sodium channel assembly and cardiac arrhythmias.

We are looking for an experienced ion channel electrophysiologist, ideally with expertise in single channel patch-clamp, general cell and molecular biological methods and a willingness to use primary animal tissues.

Candidates should have a PhD or the equivalent in a relevant science.

The work is a collaborative project between Dr A.P. Jackson and Prof. C-L. Huang (Dept. Physiology, Development and Neuroscience) and Dr A.J. Thompson (Dept. Pharmacology). It builds on recent evidence that the ß3-subunit can cross-link the heart-specific sodium channel Nav 1.5 and may help stabilise sodium channels on the plasma membranes of cardiomyocytes (see: Namadurai et al., Open Biol. 2015 5:140192. DOI: 10.1098/rsob.140192). The importance of this clustering will be investigated using cell biological and electrophysiological approaches, in primary cells derived from wild-type and ß3-knockout mice (see: Hakim, P et al., Acta. Physiol. 2010, 198, 47-59).

Fixed-term: The funds for this post are available for up to three years.

To apply online for this vacancy, please click on the 'Apply' button below. This will route you to the University's Web Recruitment System, where you will need to register an account (if you have not already) and log in before completing the online application form.

For all enquires please contact the Biochemistry Personnel - personnel@bioc.cam.ac.uk

Please quote reference PH05214 on your application and in any correspondence about this vacancy.

The University values diversity and is committed to equality of opportunity.

The University has a responsibility to ensure that all employees are eligible to live and work in the UK.

Research Associate (Fixed Term)

From Jobs at the University of Cambridge. Published on Jan 12, 2015.

A postdoctoral position is available for 12 months (with a possibility of extension) from 1st March 2015 (or later) to work with Dr Florian Hollfelder in the University of Cambridge, Department of Biochemistry, Tennis Court Road, Central Cambridge on evolution and ancestral reconstruction of highly promiscuous enzymes (namely members of the alkaline phosphatase that catalyse up to 6 chemically distinct activities). We will base directed evolution of new generalists and specialists on measurement of fitness landscapes of randomised protein populations: such distribution functions for multiple activities will be gathered by ultrahigh-throughput screening of >106 clones in picolitre droplet compartments during in vitro and in vivo evolution cycles, followed by systematic characterization of multiple fitness traits such as expression level, stability or reaction kinetics. These approaches will enable a dynamic picture of protein evolution at various levels from transition state recognition, protein biophysics to population genetics. The project involves collaboration with the groups of Erich Bornberg-Bauer at Münster (http://ieb.uni-muenster.de/bioinf/people/ebb) and Nobu Tokuriki at Vancouver (http://www.msl.ubc.ca/faculty/tokuriki).

Candidates should have skills in molecular and chemical biology, protein engineering, yeast display, library selection techniques, in vitro protein expression and kinetic analysis of enzymes or alternatively micro engineering and droplet microfluidics.

Candidates should have a PhD or the equivalent in a relevant science.

Fixed-term: The funds for this post are available for 12 months in the first instance.

To apply online for this vacancy, please click on the 'Apply' button below. This will route you to the University's Web Recruitment System, where you will need to register an account (if you have not already) and log in before completing the online application form.

Informal inquiries can be made by contacting Dr Florian Hollfelder - fh111@cam.ac.uk

Please quote reference PH05098 on your application and in any correspondence about this vacancy.

The University values diversity and is committed to equality of opportunity.

The University has a responsibility to ensure that all employees are eligible to live and work in the UK.

Research Associate (Fixed Term)

From Jobs at the University of Cambridge. Published on Jan 12, 2015.

A postdoctoral position is available for 12 months (with a possibility of extension) from 1st March 2015 (or later) to work in the group of Dr Florian Hollfelder in the University of Cambridge, Department of Biochemistry, Tennis Court Road, Central Cambridge on identification and protein engineering of protease enzymes as potential therapeutics. The project will explore the substrate promiscuity of existing proteases to adapt them for new targets and develop new therapeutic molecules. To this end directed evolution of protease will be carried out using ultra-high-throughput screening in microfluidic droplets. Kinetic and biophysical analysis will be used to analyse the mechanisms of substrate recognition of the new biocatalysts. This project is a collaboration with Drs. Nat Gordon, Lutz Jermutus and others at MedImmune, the biologics arm of AstraZeneca based in Cambridge.

Candidates should have skills in molecular and chemical biology, protein engineering and kinetic analysis of enzymes.

Candidates should have a PhD or the equivalent in a relevant science.

Fixed-term: The funds for this post are available for 12 months in the first instance.

To apply online for this vacancy, please click on the 'Apply' button below. This will route you to the University's Web Recruitment System, where you will need to register an account (if you have not already) and log in before completing the online application form.

Informal inquiries can be made by contacting Dr Florian Hollfelder - fh111@cam.ac.uk

Please quote reference PH05105 on your application and in any correspondence about this vacancy.

The University values diversity and is committed to equality of opportunity.

The University has a responsibility to ensure that all employees are eligible to live and work in the UK.

Research Associate (Fixed Term)

From Jobs at the University of Cambridge. Published on Jan 08, 2015.

Molecular Parasitology: MRC funded postdoctoral research position

Targeted investigation of the molecular composition and function of the apical complex in Toxoplasma.

Skill sets: Molecular biology, cell biology, tissue culture, microscopy.

A position is available for a post-doctoral research worker to apply state-of-the-art technologies to investigate the molecular composition and function of the apical complex in Toxoplasma gondii. Protein discovery, tagging, knockdown and knockout strategies will be employed to examine the molecular behaviour and mechanisms associated with the function of this iconic feature of apicomplexan parasites. The outcomes of this project will contribute to better understanding of the apical complex's significance to invasion, cell replication, and other aspects of apicomplexan biology.

The position will be based in the Department of Biochemistry, Tennis Court Road at the University of Cambridge (central Cambridge) in the laboratory of Dr Ross Waller.

Candidates should have a PhD in cell and molecular biology, preferably with experience in parasitology and a track record of publishing in high quality journals. Ability to work both as part of a team, and independently, coupled with excellent communication, organizational and problem solving skills are required.

The host laboratory provides cell culture, molecular biology, and wide-field fluorescence microscopy facilities, and provides access to super-resolution and electron microscopy, as well as advanced proteomics. The project is supported by a technician.

The position is available from 1st March 2015, and is funded for 3 years in the first instance.

To apply online for this vacancy, please click on the 'Apply' button below. This will route you to the University's Web Recruitment System, where you will need to register an account (if you have not already) and log in before completing the online application form.

Informal inquiries can be made by contacting Ross Waller (rfw26@cam.ac.uk).

Please quote reference PH05081 on your application and in any correspondence about this vacancy.

The University values diversity and is committed to equality of opportunity.

The University has a responsibility to ensure that all employees are eligible to live and work in the UK.

Research Data Technician (Fixed Term)

From Jobs at the University of Cambridge. Published on Jan 30, 2015.

Cambridge Centre for Proteomics (CCP) is currently looking to recruit a Research Data Technician within its core facility unit. The core facility offers proteomics services to users from the University of Cambridge and other worldwide academic and industrial groups.

The successful candidate will be required to analyse proteomics data using standard proteomic packages including Proteome Discoverer, Mascot and Scaffold and apply common statistical methods to datasets. Some degree of programming literacy is essential, preferably R/Bioconductor. The role-holder will be an integral member of CCP and will need to interact closely with other members of CCP and internal and external users of the service to provide interpretation of results from complex datasets.

The successful candidate will have a first degree in computer or biological sciences or statistics and previous experience in working with high throughput mass spectrometry data sets would be advantageous.

Fixed-term: The funds for this post are available for 2 years in the first instance.

Once an offer of employment has been accepted, the successful candidate will be required to undergo a health assessment.

To apply online for this vacancy, please click on the 'Apply' button below. This will route you to the University's Web Recruitment System, where you will need to register an account (if you have not already) and log in before completing the online application form.

For all inquiries, please contact Mike Deery, md289@cam.ac.uk.

Please quote reference PH05266 on your application and in any correspondence about this vacancy.

The University values diversity and is committed to equality of opportunity.

The University has a responsibility to ensure that all employees are eligible to live and work in the UK.